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Effect of graphene oxide with different morphological characteristics on properties of immobilized enzyme in the covalent method

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Abstract

Although graphene oxide (GO) has great potential in the field of immobilized enzyme catalysts, the detailed effects of GO with different morphological structures on immobilized enzyme are not well understood. GOs were prepared from 8000 mesh and nanoscale graphite at different reaction temperatures, and used as carriers to immobilize alpha-amylase by cross-linking method. The properties of GOs were characterized through Atomic force microscope, Fourier-transformed infrared, X-ray photoelectron spectroscopy, Raman and UV–Vis. Furthermore, the dosage of cross-linking agent, cross-linking time, optimum temperature/pH, thermal/pH/storage stability, reusability and kinetic parameters of immobilized enzymes were investigated. The results showed that the loading of alpha-amylase on GOs was 162.3–274.2 mg g−1. The reusability experiments revealed high activity maintenance of immobilized alpha-amylase even after seven reaction cycles. Moreover, the storage stability of immobilized enzyme improved via immobilization in comparison with free one and it maintained over 70% of their initial activity after 20 days storage at 4 °C.

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Acknowledgements

The project was supported by Open project of National Key Laboratory of Chemical Resource Engineering (Grants CRE-2017-C-305); Program for Innovative Research Team of Henan Polytechnic University (Grants T2018-3); The Natural Science Foundation of China (U1704127), Support plan for scientific and technological innovation team of colleges and universities in Henan Province (Grants 18IRTSTHN010).

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  1. School of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, 454000, Henan, China

    Han Zhang, Shao-Feng Hua, Cong-qiang Li, Lei Zhang, Yun-Chang Fan & Peigao Duan

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  1. Han Zhang
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Zhang, H., Hua, SF., Li, Cq. et al. Effect of graphene oxide with different morphological characteristics on properties of immobilized enzyme in the covalent method. Bioprocess Biosyst Eng 43, 1847–1858 (2020). https://doi.org/10.1007/s00449-020-02375-9

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  • DOI: https://doi.org/10.1007/s00449-020-02375-9

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