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Immobilization of Lactoperoxidase on Graphene Oxide Nanosheets and Copper Oxide Nanoparticles and Evaluation of Their Stability

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Abstract

Lactoperoxidase (LPO) is a peroxidase enzyme that functions as a natural antibacterial, antiviral, antioxidant and antitumor agent. Stabilization of LPO is a key factor in its industrial applications. In this respect, this work focused on immobilizing LPO on graphene oxide (GO) nanosheets and copper oxide (CuO) nanoparticles using glutaraldehyde, as a cross-linking reagent, and investigating its stability. The Km values of free LPO and LPO immobilized on GO (LPO–GO) and CuO (LPO–CuO) were found to be 53.19, 83.33 and 98.7 mM and their Vmax values equaled to 0.629, 0.504 and 0.41 U/mL min, respectively. The LPO–GO and LPO–CuO samples retained 35 and 12% of their primary activity within 30 days at 25 °C whereas the free enzyme lost its activity after 7 days at the same temperature. Moreover, evaluation of the thermal stability of LPO at 75 °C determined conservation of 24 and 8% of the primary activity of LPO in the LPO–GO and LPO–CuO samples, respectively, after 60 min whereas the free enzyme lost its activity after 5 min. As the findings demonstrated, GO nanosheets are more appropriate for immobilization of LPO, compared with CuO.

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Acknowledgements

The authors are grateful to the Department of Biology, Payame Noor University of Isfahan for their cooperation and supplying the experimental equipment.

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Authors and Affiliations

  1. Department of Biology, Payame Noor University, P.O. Box 19395-3697, Tehran, Iran

    Mehrnaz Movahedi & Habibollah Nazem

  2. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

    Seyed Ziyae Aldin Samsam Shariat

Authors
  1. Mehrnaz Movahedi
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  2. Seyed Ziyae Aldin Samsam Shariat
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  3. Habibollah Nazem
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Correspondence to Seyed Ziyae Aldin Samsam Shariat.

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Movahedi, M., Samsam Shariat, S.Z.A. & Nazem, H. Immobilization of Lactoperoxidase on Graphene Oxide Nanosheets and Copper Oxide Nanoparticles and Evaluation of Their Stability. Catal Lett 149, 562–573 (2019). https://doi.org/10.1007/s10562-018-2620-0

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