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Dual metal cations coated magnetic mesoporous silica probe for highly selective capture of endogenous phosphopeptides in biological samples

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Abstract

For the first time, dual metal ions (Ti4+-Zr4+) were successfully modified into the channel of magnetic mesoporous silica to obtain an affinity probe for highly selective capture of endogenous phosphopeptides in biological samples. The newly prepared Fe3O4@mSiO2@Ti4+-Zr4+ composites possessed the advantages of ordered mesoporous channels, superparamagnetism, and enhanced affinity properties of dual metal ions of Ti4+ and Zr4+. The phosphopeptide enrichment efficiency of the Fe3O4@mSiO2@Ti4+-Zr4+ composite was investigated, and the result indicated an ultrahigh size exclusive ability (weight ratio of β-casein tryptic digests, BSA, and α-casein protein reached up to 1:1000:1000). Compared to magnetic affinity probes with single metal ions (Fe3O4@mSiO2@Ti4+, Fe3O4@mSiO2@Zr4+), the composite possessed stronger specificity, higher sensitivity, and better efficiency; and more importantly, it showed much enhanced enrichment ability towards both mono- and multi-phosphorylated peptides. Additionally, by utilizing the Fe3O4@mSiO2@Ti4+-Zr4+ affinity probe, a total number of 104 endogenous phosphopeptides including 95 mono-phosphopeptides and 9 multi-phosphopeptides were captured and identified from human saliva, indicating the great potential for the application of the novel probe for the peptidome analysis in the future.

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Funding

This work was financially supported by the National Natural Science Foundation of China (21425518, 21405022, and 21675034) and National Basic Research Priorities Program of China (2013CB911201).

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Authors and Affiliations

  1. Department of Chemistry, Department of Gastroenterology and Hepatology, Zhongshan Hospital, Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China

    Xufang Hu, Yilin Li & Chunhui Deng

  2. Department of Ophthalmology and Vision Science, Eye and ENT Hospital, NHC Key Laboratory of Myopia, Fudan University, Shanghai, China

    Aizhu Miao

Authors
  1. Xufang Hu
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  2. Yilin Li
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  3. Aizhu Miao
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  4. Chunhui Deng
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Correspondence to Aizhu Miao or Chunhui Deng.

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Hu, X., Li, Y., Miao, A. et al. Dual metal cations coated magnetic mesoporous silica probe for highly selective capture of endogenous phosphopeptides in biological samples. Microchim Acta 187, 400 (2020). https://doi.org/10.1007/s00604-020-04323-6

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