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A capillary column packed with a zirconium(IV)-based organic framework for enrichment of endogenous phosphopeptides

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Abstract

A zirconium(IV)-based metal organic framework (Zr-MOF) was deposited on polydopamine-coated silica microspheres to form microspheres of type SiO2@PDA@Zr-MOF. These were packed into capillary columns for enrichment of phosphopeptides. The column was off-line coupled to both matrix-assisted laser desorption/ionization time of flight mass spectrometry and LC-ESI-MS/MS. The method has a detection limit as low as 4 fmol of β-casein digest and a selectivity as high as 1:1000 (molar ratio of β-casein and BSA digest). It was applied to the analysis of human saliva. In total, 240 endogenous phosphopeptides were identified in only 25 μL human saliva.

A zirconium-based metal organic framework (Zr-MOF) was modified outside of polydopamine-coated silica microspheres to form microspheres named SiO2@PDA@Zr-MOF. Then they were packed in capillary columns for selective enrichment of phosphopeptides via interaction between Zr-O clusters and phosphate groups. The pre-concentration resulted in a better detection of phosphopeptides by mass spectrometry. Tris: Tris(hydroxymethyl)aminomethane; DMF: Dimethyl Formamide; Zr-MOF: Zirconium(IV)-organic framework; MOAC: Metal oxide affinity chromatography.

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Acknowledgements

This work was financially supported by National Key R&D Program of China (2018YFA0507501) and the National Natural Science Foundation of China (21425518).

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Authors and Affiliations

  1. The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai, 200240, China

    Haizhu Lin, Hemei Chen, Xi Shao & Chunhui Deng

  2. Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China

    Haizhu Lin, Hemei Chen, Xi Shao & Chunhui Deng

  3. Department of Chemistry, and Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200433, China

    Haizhu Lin, Hemei Chen, Xi Shao & Chunhui Deng

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  1. Haizhu Lin
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Correspondence to Chunhui Deng.

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Lin, H., Chen, H., Shao, X. et al. A capillary column packed with a zirconium(IV)-based organic framework for enrichment of endogenous phosphopeptides. Microchim Acta 185, 562 (2018). https://doi.org/10.1007/s00604-018-3109-7

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