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Binary magnetic metal-organic frameworks composites: a promising affinity probe for highly selective and rapid enrichment of mono- and multi-phosphopeptides

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Abstract

A binary magnetic metal-organic framework (MOF)-functionalized material (magG@PDA@Ni-MOF@Fe-MOF) was prepared through grafting Ni-MOF and Fe-MOF on magnetic (Fe3O4) graphene with polydopamine (PDA) as a middle layer. Compared with single MOFs functionalized materials (magG@PDA@Ni-MOF and magG@PDA@Fe-MOF) under the same conditions, magG@PDA@Ni-MOF@Fe-MOF not only displays lower detection limits (4 fmol) and selectivity (1:1000), but also has better enrichment efficiency for both multi- and monophosphopeptides. Other than this, magG@PDA@Ni-MOF@Fe-MOF exhibits fine reusability (five cycles) and rapid enrichment property (1 min), and 24 phosphopeptides were detected when it was applied to the analysis of human saliva.

The strategy of preparing a binary magnetic metal-organic framework (MOF)-functionalized material (magG@PDA@Ni-MOF@Fe-MOF) through grafting Ni-MOF and Fe-MOF on magnetic graphene with polydopamine (PDA) as a middle layer.

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Acknowledgements

This work is supported by Zhejiang Natural Science Foundation (LQ19C050002), Ningbo Natural Science Foundation (2018A610279), and the K. C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, 315211, China

    Baichun Wang, Bin Liu, Yinghua Yan, Keqi Tang & Chuan-Fan Ding

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  1. Baichun Wang
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  2. Bin Liu
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  3. Yinghua Yan
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Correspondence to Yinghua Yan or Keqi Tang.

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Wang, B., Liu, B., Yan, Y. et al. Binary magnetic metal-organic frameworks composites: a promising affinity probe for highly selective and rapid enrichment of mono- and multi-phosphopeptides. Microchim Acta 186, 832 (2019). https://doi.org/10.1007/s00604-019-3916-5

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