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Proteomic profile of Citrus grandis roots under long-term boron-deficiency revealed by iTRAQ

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Eighty-six differentially abundant proteins were identified in Citrus grandis roots in response to boron-deficiency using the iTRAQ technique and possible mechanism underlying boron-deficiency tolerance of citrus plants was identified.

Abstract

Boron (B) is an essential element for plant growth and development and adequate B supply is an important determinant of good quality and high yield of crops. B-deficiency is a worldwide problem in agricultural production including citrus. However, little is known about the molecular mechanism of plant tolerance to B-deficiency. Using the iTRAQ technique, 86 differentially abundant proteins were identified from B-deficient Citrus grandis roots. The adaptive strategy of C. grandis roots under B-deficiency was summarized as follows: (1) enhancement of alternative splicing of mRNA and DNA methylation; (2) up-regulation of post-translation modification (PTM) and turnover of proteins; (3) reinforcement of cellular transport; (4) enhancement of antioxidant system and signal transduction. In general, these results increase our understanding of molecular mechanisms underlining the resistance of citrus plant under B-deficiency. Further studies should focus on how do roots perceive B deficiency in the rhizosphere and which pathway or proteins react to this adverse condition in the first place and then stimulates the downstream responses in Citrus plants.

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Acknowledgments

This study was financially supported by the earmarked fund for China Agriculture Research System (No. CARS-27), National Natural Science Grant of China (No. 31301740) and the Provincial Natural Science Grant of Fujian, China (No. 2014J05033). The funding authorities had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. College of Resource and Environmental Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Lin-Tong Yang, Yi-Bin Lu, Yuan Zhang, Peng Guo & Li-Song Chen

  2. Institute of Plant Nutritional and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Lin-Tong Yang, Yi-Bin Lu, Yuan Zhang, Peng Guo & Li-Song Chen

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  1. Lin-Tong Yang
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Correspondence to Li-Song Chen.

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Communicated by E. Magel.

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Yang, LT., Lu, YB., Zhang, Y. et al. Proteomic profile of Citrus grandis roots under long-term boron-deficiency revealed by iTRAQ. Trees 30, 1057–1071 (2016). https://doi.org/10.1007/s00468-015-1344-7

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