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Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stresses

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Abstract

Glyoxalase pathway, ubiquitously found in all organisms from prokaryotes to eukaryotes, consists of glyoxalase I (GLY I) and glyoxalase II (GLY II) enzymes, which detoxify a cytotoxic molecule, methylglyoxal (MG). Increase in MG has been correlated with various diseases in humans and different abiotic stresses in plants. We have previously shown that overproduction of GLY I and/or GLY II enzymes in transgenic plants provide tolerance towards salinity and heavy metal stresses. We have identified nineteen potential GLY I and four GLY II proteins in rice and twenty two GLY I and nine GLY II proteins in Arabidopsis. An analysis of complete set of genes coding for the glyoxalase proteins in these two genomes is presented, including classification and chromosomal distribution. Expression profiling of these genes has been performed in response to multiple abiotic stresses, in different tissues and during various stages of vegetative and reproductive development using publicly available databases (massively parallel signature sequencing and microarray). AtGLYI8, OsGLYI3, and OsGLYI10 expresses constitutively high in seeds while AtGLYI4, AtGLYI7, OsGLYI6, and OsGLYI11 are highly stress inducible. To complement this analyses, qRT-PCR is performed in two contrasting rice genotypes, i.e., IR64 and Pokkali where OsGLYI6 and OsGLYI11 are found to be highly stress inducible.

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Acknowledgments

The authors thankfully acknowledge the research funds received from International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi. SLS-P thanks award of Innovative Young Biotechnologist award (IYBA) of Department of Biotechnology (DBT), Government of India. AM thanks ICGEB for providing pre-doctoral fellowship. Authors would also like to thank Dr. Sanjay Kapoor, University of Delhi South Campus, New Delhi, India, for providing help in retrieving rice microarray data and Professor RC Pant for the critical reading of the manuscript.

Conflicts of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Plant Molecular Biology, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Ananda Mustafiz, Anil Kumar Singh, Sudhir Kumar Sopory & Sneh Lata Singla-Pareek

  2. Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Ashwani Pareek

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  1. Ananda Mustafiz
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  2. Anil Kumar Singh
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  3. Ashwani Pareek
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  4. Sudhir Kumar Sopory
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  5. Sneh Lata Singla-Pareek
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Correspondence to Sneh Lata Singla-Pareek.

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Mustafiz, A., Singh, A.K., Pareek, A. et al. Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stresses. Funct Integr Genomics 11, 293–305 (2011). https://doi.org/10.1007/s10142-010-0203-2

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  • DOI: https://doi.org/10.1007/s10142-010-0203-2

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