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Mucilaginibacter terrigena sp. nov. sp., A Novel Member of the Family Sphingobacteriaceae

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Abstract

A bacterial strain, 17JY9-4T, was isolated from a soil sample collected on Jeju Island, South Korea. Colonies grown on R2A agar are pale pink in color, and cells are Gram-stain negative, short, and rod-shaped. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Mucilaginibacter in the family Sphingobacteriaceae, with high levels of 16S rRNA sequence similarity shared with Mucilaginibacter lutimaris BR-3T (98.0%), Mucilaginibacter rigui WPCB133T (98.0%), Mucilaginibacter phyllosphaerae PP-F2F-G21T (97.0%), Mucilaginibacter amnicola TAPP7T (96.8%), and Mucilaginibacter soli R9-65T (96.7%). Growth of strain 17JY9-4T occurs at 10–30 °C, pH 6–8, and in the presence of 0–1.0% NaCl. The genomic G+C content is 44.38 mol%. The predominant respiratory quinone of the isolate is MK-7; the major fatty acids are summed feature 3 (C16:1ω7c/C16:1ω6c) (39.7%), iso-C15:0 (22.8%), iso-C17:0 3-OH (7.8%), and C16:0 (7.7%); and the major polar lipid is phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the placement of strain 17JY9-4T within the genus Mucilaginibacter. However, the DNA–DNA relatedness between the isolate and M. rigui, M. lutimaris, M. phyllosphaerae, M. amnicola, and M. soli were 44.3 ± 3.0%, 38.6 ± 3.7%, 23.2 ± 2.9%, 21.9 ± 3.1%, and 18.6 ± 3.7%, respectively. The results of 16S rRNA gene sequence similarity analysis, DNA–DNA hybridization analysis, and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17JY9-4T represents a novel species in the genus Mucilaginibacter, for which the name Mucilaginibacter terrigena sp. nov. is proposed. The type strain is 17JY9-4T (= KCTC 62294T = JCM 33049T).

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Acknowledgements

This work was supported by the Brain Pool Program (Grant No. 2019H1D3A2A01061406) through the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Leonid N. Ten, Na Yeong Jeon, Weilan Li, Seung-Yeol Lee & Hee-Young Jung

  2. School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea

    Young-Je Cho

  3. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim

  4. Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL, 61604, USA

    Alejandro P. Rooney & Hee-Young Jung

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The 16S rRNA gene sequence of strain 17JY9-4T has been deposited in NCBI GenBank/EMBL/DDBJ under the accession number LC434629. The Whole Genome Shotgun project number of strain 17JY9-4T in GenBank/EMBL/DDBJ is SEWG00000000. The Digital Protologue Database TaxoNumber for strain 17JY9-4T is TA00831.

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Ten, L.N., Jeon, N.Y., Li, W. et al. Mucilaginibacter terrigena sp. nov. sp., A Novel Member of the Family Sphingobacteriaceae. Curr Microbiol 76, 1152–1160 (2019). https://doi.org/10.1007/s00284-019-01748-y

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