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Sphingomonas parva sp. nov., isolated from soil in Jeju Island

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Abstract

A Gram staining-negative, yellow-colored, rod-shaped, non-motile and aerobic, designated strain 17J27-24T was isolated from a soil sample in Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 17J27-24T was related to the members of the family Sphingomonadaceae and formed a distinct monophyletic cluster within the genus Sphingomonas with Sphingomonas deserti (98.3% 16S rRNA gene sequence similarity). Growth was observed at 30 °C (optimum), at pH 7.0 (optimum), and in the absence of NaCl (%). The predominant cellular fatty acids were summed feature 8 (18:1 ω7c and/or 18:1 ω6c) and C17:1 ω6c. The major respiratory quinone was Q10. The polar lipids profile comprised of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and sphingoglycolipid (SGL). The DNA G + C content was 77.8 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values between 17J27-24T and its phylogenetically closest Sphingomonas deserti (KCTC 62411T) were below the established cut-off < 94% (ANI) and < 70% (dDDH) for species delineation. Moreover, the results of the polyphasic approach confirmed that strain 17J27-24T represents a novel species of the genus Sphingomonas within the family Sphingomonadaceae, for which the name Sphingomonas parva sp. nov. is proposed. The type strain of this species is 17J27-24T (= KCTC 62208T = JCM 3896T). An emended description of the species Sphingomonas parva is provided.

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Acknowledgements

This research was supported by the MISP (Ministry of Science, ICT & Future Planning), Korea, under the National Program for Excellence in SW supervised by the IITP (Institute of Information & communications Technology Planning & Evaluation)” (2016-0-00022) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201801204).

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Authors and Affiliations

  1. Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, South Korea

    Soohyun Maeng, Yuna Park, Ju Hyun Han, Sung Eun Lee, Chang-woo Ha, Myung Kyum Kim & Gayathri Subramani

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  1. Soohyun Maeng
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  2. Yuna Park
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  3. Ju Hyun Han
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  4. Sung Eun Lee
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  5. Chang-woo Ha
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  6. Myung Kyum Kim
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Correspondence to Myung Kyum Kim or Gayathri Subramani.

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Communicated by Erko Stackebrandt .

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Nucleotide Accession number: The GenBank accession number for 16S rRNA gene sequence of strain 17J27-24T is MK064225. The accession number for the draft genome sequence of strain 17J27-24T is NZ_SPDV00000000.

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Maeng, S., Park, Y., Han, J.H. et al. Sphingomonas parva sp. nov., isolated from soil in Jeju Island. Arch Microbiol 202, 2429–2436 (2020). https://doi.org/10.1007/s00203-020-01925-9

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