Abstract
Pectinase, a group of pectin degrading enzymes, is one of the most influential industrial enzymes, helpful in producing a wide variety of products with good qualities. These enzymes are biocatalysts and are highly specific, non-toxic, sustainable, and eco-friendly. Consequently, both pectin and pectinase are crucially essential biomolecules with extensive applicatory perception in the biotechnological sector. The market demand and application of pectinases in new sectors are continuously increasing. However, due to the high cost of the substrate used for the growth of microbes, the production of pectinase using microorganisms is limited. Therefore, low-cost or no-cost substrates, such as various agricultural biomasses, are emphasized in producing pectinases. The importance and implications of pectinases are rising in diverse areas, including bioethanol production, extraction of DNA, and protoplast isolation from a plant. Therefore, this review briefly describes the structure of pectin, types and source of pectinases, substrates and strategies used for pectinases production, and emphasizes diverse potential applications of pectinases. The review also has included a list of pectinases producing microbes and alternative substrates for commercial production of pectinase applicable in pectinase-based industrial technology.
Key points
• Pectinase applications are continuously expanding.
• Organic wastes can be used as low-cost sources of pectin.
• Utilization of wastes helps to reduce pollution.
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This work was supported by the Natural Sciences and Engineering Research Council of Canada Funding (RGPIN-2017–05366) to Wensheng Qin and Ontario Graduate Scholarship to Sarita Shrestha.
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SS did the major contribution in designing and writing the manuscript. WQ did proofread, and all authors helped in writing and editing. All authors read and approved the final manuscript before submission.
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Shrestha, S., Rahman, M.S. & Qin, W. New insights in pectinase production development and industrial applications. Appl Microbiol Biotechnol 105, 9069–9087 (2021). https://doi.org/10.1007/s00253-021-11705-0
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DOI: https://doi.org/10.1007/s00253-021-11705-0