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Microbial community composition and dynamics in high-temperature biogas reactors using industrial bioethanol waste as substrate

  • Environmental biotechnology
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Abstract

Stillage, which is generated during bioethanol production, constitutes a promising substrate for biogas production within the scope of an integrated biorefinery concept. In this study, a microbial community was grown on thin stillage as mono-substrate in a continuous stirred tank reactor (CSTR) at a constant temperature of 55 °C, at an organic loading rate of 1.5 goTS/L*d and a retention time of 25 days. Using an amplicon-based dataset of 17,400 high-quality sequences of 16S rRNA gene fragments (V2–V3 regions), predominance of Bacteria assigned to the families Thermotogaceae and Elusimicrobiaceae was detected. Dominant members of methane-producing Euryarchaeota within the CSTR belonged to obligate acetoclastic Methanosaetaceae and hydrogenotrophic Methanobacteriaceae. In order to investigate population dynamics during reactor acidification, the organic loading rate was increased abruptly, which resulted in an elevated concentration of volatile fatty acids. Acidification led to a decrease in relative abundance of Bacteria accompanied with stable numbers of Archaea. Nevertheless, the abundance of Methanosaetaceae increased while that of Methanobacteriales decreased successively. These findings demonstrate that a profound intervention to the biogas process may result in persistent community changes and reveals uncommon bacterial families as process-relevant microorganisms.

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Acknowledgments

This project was funded by the German Federal Ministry for Education and Research (BMBF) through the Project Management Jülich (PtJ) as part of the Cluster Biorefinery2021 (grant no. 0315559A). I. Röske and W. Sabra gratefully acknowledge the receipt of a scholarship and the SynBio grant from the State Excellence Initiative Hamburg.

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Authors and Affiliations

  1. Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstraße 12, Hamburg, 21073, Germany

    Immo Röske, Garabed Antranikian & Kerstin Sahm

  2. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany

    Wael Sabra & An-Ping Zeng

  3. Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt

    Wael Sabra

  4. Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University, Göttingen, Germany

    Heiko Nacke & Rolf Daniel

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  1. Immo Röske
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Röske, I., Sabra, W., Nacke, H. et al. Microbial community composition and dynamics in high-temperature biogas reactors using industrial bioethanol waste as substrate. Appl Microbiol Biotechnol 98, 9095–9106 (2014). https://doi.org/10.1007/s00253-014-5906-1

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