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FISH in Lampbrush Chromosomes

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Fluorescence In Situ Hybridization (FISH)

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Abstract

Conventional protocols of fluorescence in situ hybridization (FISH) usually imply the use of mitotic metaphase chromosomes obtained from somatic cells. In contrast, here we provide a protocol of FISH on giant lampbrush chromosomes (LBCs) that represent meiotic diplotene half- bivalents from growing oocytes and can be found in many species including amphibians and birds. Due to the peculiarities of their organization, LBCs can serve as a relevant model for high-resolution molecular cytogenetic and cytological investigations. In this chapter we describe the basic protocol of FISH on LBCs that allows revealing both DNA and nascent RNA-transcript targets. Additionally, key variations in hybridization protocol and the examples of their application are also outlined.

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Acknowledgments

The authors would like to thank Dmitry Dedukh (St. Petersburg State University) for the images presented in Fig. 1a, b. The research was supported by Russian Science Foundation (grant #14-14-00131). The work was partially performed using experimental equipment of the Environmental Safety Observatory and the Research Resource Centers “Chromas” and “Molecular and Cell Technologies” of St. Petersburg State University.

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Authors and Affiliations

  1. Saint Petersburg State University, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia

    Anna Zlotina & Alla Krasikova

Authors
  1. Anna Zlotina
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  2. Alla Krasikova
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Corresponding author

Correspondence to Alla Krasikova .

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Editors and Affiliations

  1. Institut für Humangenetik, Jena, Germany

    Thomas Liehr

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© 2017 Springer-Verlag Berlin Heidelberg

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Zlotina, A., Krasikova, A. (2017). FISH in Lampbrush Chromosomes. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_45

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  • DOI: https://doi.org/10.1007/978-3-662-52959-1_45

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-52957-7

  • Online ISBN: 978-3-662-52959-1

  • eBook Packages: Springer Protocols

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