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Transcription on lampbrush chromosomes of a centromerically localized highly repeated DNA in pigeon (Columba) relates to sequence arrangement

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Abstract

A highly repetitive, centromerically localized DNA sequence (PR1) has been isolated from the genomic DNA of two species of pigeon (Columba livia andC. palumbus). PR1 is approximately 900 bp long. It includes a sequence that is similar to the CENP-B box of mammals. It represents about 5% of the genome inC. livia and 2% inC. palumbus. In both species, tandem arrays ofPR1 form part of larger repeating units. The organization of PR1 repeats and the larger repeating units is strikingly different in the two species. The large repeating units inC. livia include long (at least 14 units) tandem arrays of PR1 interspersed with relatively short intervening sequences. The large repeats ofC. palumbus have much shorter (4 units or fewer) PR1 arrays interspersed with longer sections of non-PR1 DNA. PR1 is transcribed on short lampbrush loops in the centromeric regions of all lampbrush bivalents ofC. palumbus. InC. livia, it is not transcribed at any of the major pericentromeric sites at which it is known to be present, although it is transcribed at one minor centromeric site on chromosome 2. It is proposed that transcription of the noncoding PR1 sequence on lampbrush chromosomes of pigeons relates to its genomic organization. The proposal is discussed with regard to the ‘read-through’ hypothesis for transcription on lampbrush loops.

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Authors and Affiliations

  1. Department of Zoology, University of Leicester, LE1 7RH, Leicester, UK

    H. C. Macgregor

  2. Biological Institute, St Petersburg University, St Petersburg, Russia

    I. V. Solovei & E. R. Gaginskaya

  3. Zoological Institute, Russian Academy of Sciences, St Petersburg, Russia

    B. I. Joffe

Authors
  1. I. V. Solovei
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  2. B. I. Joffe
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  3. E. R. Gaginskaya
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  4. H. C. Macgregor
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accepted for publication by M. Schmid

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Solovei, I.V., Joffe, B.I., Gaginskaya, E.R. et al. Transcription on lampbrush chromosomes of a centromerically localized highly repeated DNA in pigeon (Columba) relates to sequence arrangement. Chromosome Res 4, 588–603 (1996). https://doi.org/10.1007/BF02261722

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  • DOI: https://doi.org/10.1007/BF02261722

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