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Grapevine (Vitis spp.)

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Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 85))

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Abstract

Grapevine somatic embryogenesis is a precious tool in breeding programs as well as in functional genomics studies, embryo tissues being the best sources for regeneration of genetically modified plants. It has also been proposed as a strategy aimed at induction of somaclonal variation, separation of periclinal chimeras, cryopreservation, virus eradication. A reliable technique for the production of somatic embryos and regenerated plantlets from an ample number of Vitis genotypes is an essential pre-requisite for any application of somatic embryogenesis. Main developmental phases can be identified: (a) Induction of callogenesis and embryogenic competence in cultured explants; (b) Culture of embryogenic calli and expression of the embryogenic program; (c) Long term culture of embryogenic callus and preservation of the embryogenic ability of the cultures or re-initiation of embryogenic calli from somatic embryos; (d) Development of somatic embryos into plantlets. The protocol for grapevine plant regeneration via somatic embryogenesis described in this chapter proved efficient for many cultivars; details are also given on the steps requiring further protocol refinements.

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Authors and Affiliations

  1. Institute for Sustainable Plant Protection, National Research Council of Italy, Strada delle Cacce 73, 10135, Turin, Italy

    Ivana Gribaudo & Giorgio Gambino

Authors
  1. Ivana Gribaudo
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  2. Giorgio Gambino
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Correspondence to Ivana Gribaudo .

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Editors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Shri Mohan Jain

  2. Federal Way, Washington, USA

    Pramod Gupta

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Gribaudo, I., Gambino, G. (2018). Grapevine (Vitis spp.). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-319-79087-9_12

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