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Molecular characterization of grapevine plants transformed with GFLV resistance genes: I

  • Genetic Transformation and Hybridization
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Abstract

The Grapevine FanLeaf Virus-Coat Protein (GFLV CP) gene was inserted through Agrobacterium-mediated transformation in Vitis vinifera ‘Nebbiolo’, ‘Lumassina’ and ‘Blaufränkisch’. Two plasmids were used: pGA-CP+ (full-length GFLV CP gene with an introduced start codon) and pGA-AS (same gene in antisense orientation). Forty-three transgenic lines were regenerated. As several lines in Southern blots share same hybridization patterns, eight independent line groups resulted for ‘Nebbiolo’, one for ‘Lumassina’, and two for ‘Blaufränkisch’. Inserted T-DNA copies ranged from one to three; one line probably contains an incomplete copy of T-DNA. Except for one ‘Nebbiolo’ line, no evidence for methylation of the transgene at cytosine residues was found by Southern analyses. Specific mRNA was present at variable expression levels; some lines accumulated the coat protein while in others the protein was not detectable by ELISA.

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Acknowledgments

Authors wish to thank Prof. Andrea Schubert of the University of Torino, Dipartimento Colture Arboree, for his support throughout the years, and Dr. Valentina Scariot for her contribution in transgenic plant production

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Authors and Affiliations

  1. Istituto Virologia Vegetale C.N.R.—Unità staccata Viticoltura-Grugliasco, Via Leonardo da Vinci, 44, Grugliasco (TO), I-10095, Italy

    Giorgio Gambino & Ivana Gribaudo

  2. Plant Biotechnology Unit, Institute of Applied Microbiology BOKU, Wien, Austria

    Stephan Leopold, Angelica Schartl & Margit Laimer

Authors
  1. Giorgio Gambino
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  2. Ivana Gribaudo
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  3. Stephan Leopold
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  4. Angelica Schartl
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  5. Margit Laimer
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Corresponding author

Correspondence to Ivana Gribaudo.

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Communicated by H. Lörz

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Gambino, G., Gribaudo, I., Leopold, S. et al. Molecular characterization of grapevine plants transformed with GFLV resistance genes: I. Plant Cell Rep 24, 655–662 (2005). https://doi.org/10.1007/s00299-005-0006-4

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  • DOI: https://doi.org/10.1007/s00299-005-0006-4

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