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Isolation of Bacteriophages

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Bacteriophages

Abstract

Before any phage can be studied, or used for its biological properties, it must first be isolated. As such, isolation is a critical step – indeed, the critical step – in many explorations of phage biology and biotechnology. There are several techniques, both classical and modern, by which phages can be isolated, and selection of the proper method often depends on the intended use of the phage. In this chapter, we discuss the general principles of phage isolation and techniques to obtain pure phage isolates from a variety of sources, with a particular focus on the isolation of therapeutic phages.

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Authors and Affiliations

  1. Ghent University, Ghent, Belgium

    Frits van Charante

  2. Laboratory of Gene Technology, Leuven, Belgium

    Dominique Holtappels & Bob Blasdel

  3. Vésale Pharma, Noville-Sur-Mehaigne, Belgium

    Bob Blasdel

  4. Evolution Biotechnologies, Sharnbrook, UK

    Ben Burrowes

Authors
  1. Frits van Charante
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  2. Dominique Holtappels
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  3. Bob Blasdel
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  4. Ben Burrowes
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Correspondence to Ben Burrowes .

Editor information

Editors and Affiliations

  1. Evolution Biotechnologies, Milton Keynes, UK

    David R. Harper

  2. Dept. of Microbiology, The Ohio State University Dept. of Microbiology, Mansfield, OH, USA

    Stephen T. Abedon

  3. GeneWEAVE Biosciences, Roche Molecular Systems GeneWEAVE Biosciences, Los Gatos, AR, USA

    Benjamin H. Burrowes

  4. Evolution Biotechnologies, Milton Keynes, UK

    Malcolm L. McConville

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van Charante, F., Holtappels, D., Blasdel, B., Burrowes, B. (2019). Isolation of Bacteriophages. In: Harper, D., Abedon, S., Burrowes, B., McConville, M. (eds) Bacteriophages. Springer, Cham. https://doi.org/10.1007/978-3-319-40598-8_14-1

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