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Two Novel Bacteriophages of Thermophilic Bacteria Isolated from Deep-Sea Hydrothermal Fields

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Abstract

Bacteriophages of thermophiles are of great interest due to their important roles in many biogeochemical and ecological processes. However, no virion has been isolated from deep-sea thermophilic bacteria to date. In this investigation, two lytic bacteriophages (termed Bacillus virus W1 and Geobacillus virus E1) of thermophilic bacteria were purified from deep-sea hydrothermal fields in the Pacific for the first time. Bacillus virus W1 (BVW1) obtained from Bacillus sp. w13, had a long tail (300nm in length and 15 nm in width) and a hexagonal head (70 nm in diameter). Another virus, Geobacillus virus E1 (GVE1) from Geobacillus sp. E26323, was a typical Siphoviridae phage with a hexagonal head (130 nm in diameter) and a tail (180 nm in length and 30 nm in width). The two phages contained double-stranded genomic DNAs. The genomic DNA sizes of BVW1 and GVE1 were estimated to be about 18 and 41 kb, respectively. Based on SDS-PAGE of purified virions, six major proteins were revealed for each of the two phages. The findings in our study will be very helpful to realize the effect of virus on thermophiles as well as the communities in deep-sea hydrothermal fields.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (40576076) and China Ocean Mineral Resources R & D Association (DY105-02-04-05).

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Authors and Affiliations

  1. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, The People’s Republic of China

    Bin Liu & Lianhui Xie

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, The People’s Republic of China

    Bin Liu, Suijie Wu, Qing Song & Xiaobo Zhang

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  1. Bin Liu
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  2. Suijie Wu
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  3. Qing Song
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  4. Xiaobo Zhang
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  5. Lianhui Xie
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Correspondence to Xiaobo Zhang.

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Liu, B., Wu, S., Song, Q. et al. Two Novel Bacteriophages of Thermophilic Bacteria Isolated from Deep-Sea Hydrothermal Fields. Curr Microbiol 53, 163–166 (2006). https://doi.org/10.1007/s00284-005-0509-9

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  • DOI: https://doi.org/10.1007/s00284-005-0509-9

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