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Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus

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Abstract

GBK2 is a bacteriophage, isolated from a backyard compost pile, that infects the thermophile Geobacillus kaustophilus. GBK2 has a circularly permuted genome of 39,078 bp with a G+C content of 43 %. Annotation of the genome reveals 62 putative open reading frames (ORFs), 25 of which (40.3 %) show homology to known proteins and 37 of which (59.7 %) are proteins with unknown functions. Twelve of the identified ORFs had the greatest homology to genes from the phage SPP1, a phage that infects the mesophile Bacillus subtilis. The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority of GBK2 SPP1-like genes coding for proteins involved in DNA replication and metabolism.

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Acknowledgments

The authors would like to thank the Genomic Sciences Laboratory (GSL) at NCSU for assistance in sequence determination and assembly.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Campbell University, PO Box 1090, Buies Creek, NC, 27506, USA

    Timothy J. Marks

  2. Department of Plant and Microbial Biology, North Carolina State University, 4544A Thomas Hall, Campus Box 7615, Raleigh, NC, 27695, USA

    Timothy J. Marks & Paul T. Hamilton

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  1. Timothy J. Marks
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  2. Paul T. Hamilton
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Correspondence to Paul T. Hamilton.

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Marks, T.J., Hamilton, P.T. Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus . Arch Virol 159, 2771–2775 (2014). https://doi.org/10.1007/s00705-014-2101-8

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  • DOI: https://doi.org/10.1007/s00705-014-2101-8

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