Abstract
Widespread defective lysogeny was detected in Alcaligenes eutrophus by electron microscopic analysis of cultures. Mitomycin C treatment of the cultures resulted in the production of defective (inco-) particles. Polysheaths were produced both with and without induction.
With the simultaneous isolation technique six phages were isolated for hydrogen-oxidizing strains of the new species Pseudomonas pseudoflava. The phages were able to replicate under autotrophic conditions and were found to have a very restricted host range. Electron microscopic analysis allowed classification into two structural groups. Group I contained phages with contractile tails; group II contained phages with flexible, noncontractile tails. All but one (gb) of the new phages were shown to be temperate by isolation of lysogens and induction with mitomycin C.
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Abbreviations
- OD:
-
optical density
- SM-medium:
-
standard mineral medium
- KS-medium:
-
Kaserer medium
- NB:
-
nutrient broth
- p.f.u.:
-
plaque forming unit
- c.f.u.:
-
colony forming unit
- e.o.p.:
-
efficiency of plating
- Do-phage buffer:
-
Dopatka-phage buffer
- PTA:
-
phosphotungstic acid
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Auling, G., Mayer, F. & Schlegel, H.G. Isolation and partial characterization of normal and defective bacteriophages of gram-negative hydrogen bacteria. Arch. Microbiol. 115, 237–247 (1977). https://doi.org/10.1007/BF00446448
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DOI: https://doi.org/10.1007/BF00446448