Abstract
The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Carlson, K. 2005. Working with bacteriophages: Common techniques and methodological approaches., In E. Kutter and A. Sulakvelidze (Eds.), Bacteriophages: Biology and Applications. CRC Press, Baco Raton, FL.
Ackermann, H.-W. 2005. Electron micro-scopy., In E. Kutter and A. Sulakvelidze (Eds.), Bacteriophages: Biology and Applications. CRC Press, Boca Ratan, FL.
Adams, M.D. 1959. Bacteriophages. Interscience Publishers, Inc., New York.
Carlson, K. 2005. Working with bacteriophages: Common techniques and methodological approaches., In E. Kutter and A. Sulakvelidze (Eds.), Bacteriophages: Biology and Applications. CRC Press, Boca Ratan, FL.
Edelman, D.C. and J. Barletta. 2007. Real-time PCR provides improved detection and titer determination of bacteriophage. BioTechniques 35:368–375.
Gratia, A. 1936. Des relations numeriques entre bactéries lysogenes et particules de bactériophage. Annales de l’Institut Pasteur 57:652–676.
Gratia, J.-P. 2000. André Gratia: A forerunner in microbial and viral genetics. Genetics 156:471–476.
Sambrook, J. and D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.
Haberer, K. and J. Maniloff. 1982. Adsorption of the tailed mycoplasma virus L3 to cell membranes. Journal of Virology 41:501–507.
Landry, E.F. and R.M. Zsigray. 1980. Effects of calcium on the lytic cycle of Bacillus subtilis phage 41c. Journal of General Virology 51:125–135.
Mahony, D.E., P.D. Bell, and K.B. Easterbrook. 1985. Two bacteriophages of Clostridium difficile. Journal of Clinical Microbiology 21:251–254.
Sechter, I. and C.B. Gerichter. 1968. Phage typing scheme for Salmonella braenderup. Applied Microbiology 16:1708–1712.
Hongo, M. and A. Murata. 1965. Bacteriophages of Clostridium saccharoperbutylacetonicum. II. Enumeration of phages by application of the plaque-count technique and some factors influencing the plaque formation. Agricultural and Biological Chemistry 29: 1140–1145.
Lillehaug, D. 1997. An improved plaque assay for poor plaque-producing temperate lactococal bacteriophages. Journal of Applied Microbiology 83:85–90.
Mullan, M.W.A. 1979. Lactic streptococcal bacteriophage enumeration. A review of factors affecting plaque formation. Dairy Industries International 44:11–14.
Pattee, P.A. 1966. Use of tetrazolium for improved resolution of bacteriophage plaques. Journal of Bacteriology 92:787–788.
Fraser, D. and J. Crum. 1975. Enhancement of Mycoplasma virus plaque visibility by tetrazolium. Applied Microbiology 29:305–306.
Hurst, C.J., J.C. Blannon, R.L. Hardaway, and W.C. Jackson. 1994. Differential effect of tetrazolium dyes upon bacteriophage plaque assay titres. Applied & Environmental Microbiology 60:3462–3465.
Qanber, A.A. and J. Douglas. 1976. Enhancement of plaque size of a staphylococcal phage. Journal of Applied Bacteriology 40:109–110.
McConnell, M. and A. Wright. 1975. An anaerobic technique for increasing bacteriophage plaque size. Virology 65:588–590.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Kropinski, A.M., Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P. (2009). Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-60327-164-6_7
Publisher Name: Humana Press
Print ISBN: 978-1-58829-682-5
Online ISBN: 978-1-60327-164-6
eBook Packages: Springer Protocols