Abstract
The glucosinolates produced by plants of the order Brassicales are part of a potent activated chemical defense system. These nitrogen- and sulfur-containing glucosides are hydrolyzed by myrosinases upon tissue damage, forming a toxic mixture of compounds consisting mostly of the corresponding isothiocyanates and nitriles. While humans find these compounds pleasantly spicy and beneficial to health, many of them are noxious and deterrent towards microorganisms and insect herbivores. Nonetheless, ingenious and efficient biochemical mechanisms employed by several insect herbivores enable these to feed on glucosinolate-producing plants, circumventing the effects of these plant defenses. Here, we summarize some of the counteradaptations utilized by insects to overcome the defense imposed by these compounds and their hydrolysis products. Insects can divert hydrolysis to less toxic products or desulfate the parent glucosinolates to preclude them from being hydrolyzed by myrosinases. Once hydrolysis occurs, toxic electrophilic hydrolysis products can be conjugated to glutathione and various amino acids. Another insect strategy is the rapid sequestration of ingested glucosinolates to prevent hydrolysis and allow them to be used in their own defense.
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References
Kliebenstein DJ (2004) Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant Cell Environ 27:675–684
Kliebenstein DJ, Rowe HC, Denby KJ (2005) Secondary metabolites influence Arabidopsis/Botrytis interactions: variation in host production and pathogen sensitivity. Plant J 44:25–36
Wink M (1988) Plant-breeding—importance of plant secondary metabolites for protection against pathogens and herbivores. Theor Appl Genet 75:225–233
Hartmann T (2007) From waste products to ecochemicals: fifty years research of plant secondary metabolism. Phytochemistry 68:2831–2846
Fränkel GS (1959) Raison d’être of secondary plant substances. Science 129:1466–1470
Wittstock U, Kliebenstein DJ, Lambrix V, Reichelt M, Gershenzon J (2003) Glucosinolate hydrolysis and its impact on generalist and specialist insect herbivores. Recent Adv Phytochem 37:101–125
Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56:5–51
Agerbirk N, Olsen CE (2012) Glucosinolate structures in evolution. Phytochemistry 77:16–45
Clarke DB (2010) Glucosinolates, structures and analysis in food. Anal Methods 2:310–325
Halkier BA, Gershenzon J (2006) Biology and biochemistry of glucosinolates. Annu Rev Plant Biol 57:303–333
Sønderby IE, Geu-Flores F, Halkier BA (2010) Biosynthesis of glucosinolates—gene discovery and beyond. Trends Plant Sci 15:283–290
Halkier BA, Du LC (1997) The biosynthesis of glucosinolates. Trends Plant Sci 2:425–431
Benderoth M, Pfalz M, Kroymann J (2009) Methylthioalkylmalate synthases: genetics, ecology and evolution. Phytochem Rev 8:255–268
Geu-Flores F, Olsen CE, Halkier BA (2009) Towards engineering glucosinolates into non-cruciferous plants. Planta 229:261–270
Beekwilder J, van Leeuwen W, van Dam NM, Bertossi M, Grandi V, Mizzi L, Soloviev M, Szabados L, Molthoff JW, Schipper B, Verbocht H, de Vos RCH, Morandini P, Aarts MGM, Bovy A (2008) The impact of absence of aliphatic glucosinolates on insect herbivory in Arabidopsis. PLoS One 3, e2068
Müller R, de Vos M, Sun JY, Sønderby IE, Halkier BA, Wittstock U, Jander G (2010) Differential effects of indole and aliphatic glucosinolates on lepidopteran herbivores. J Chem Ecol 36:905–913
Brader G, Mikkelsen MD, Halkier BA, Palva ET (2006) Altering glucosinolate profiles modulates disease resistance in plants. Plant J 46:758–767
Schramm K, Vassão DG, Reichelt M, Gershenzon J, Wittstock U (2012) Metabolism of glucosinolate-derived isothiocyanates to glutathione conjugates in generalist lepidopteran herbivores. Insect Biochem Mol Biol 42:174–182
Rodman JE, Soltis PS, Soltis DE, Sytsma KJ, Karol KG (1998) Parallel evolution of glucosinolate biosynthesis inferred from congruent nuclear and plastid gene phylogenies. Am J Bot 85:997–1006
Mithen R, Bennett R, Marquez J (2010) Glucosinolate biochemical diversity and innovation in the Brassicales. Phytochemistry 71:2074–2086
Pedras MSC, Okinyo DPO (2008) Remarkable incorporation of the first sulfur containing indole derivative: another piece in the biosynthetic puzzle of crucifer phytoalexins. Org Biomol Chem 6:51–54
Pedras MSC, Yaya EE, Glawischnig E (2011) The phytoalexins from cultivated and wild crucifers: chemistry and biology. Nat Prod Rep 28:1381–1405
Pedras MSC, Yaya EE, Hossain S (2010) Unveiling the phytoalexin biosynthetic puzzle in salt cress: unprecedented incorporation of glucobrassicin into wasalexins A and B. Org Biomol Chem 8:5150–5158
Brown PD, Tokuhisa JG, Reichelt M, Gershenzon J (2003) Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana. Phytochemistry 62:471–481
Hopkins RJ, van Dam NM, van Loon JJ (2009) Role of glucosinolates in insect-plant relationships and multitrophic interactions. Annu Rev Entomol 54:57–83
Kliebenstein DJ, Kroymann J, Brown P, Figuth A, Pedersen D, Gershenzon J, Mitchell-Olds T (2001) Genetic control of natural variation in Arabidopsis glucosinolate accumulation. Plant Physiol 126:811–825
Textor S, Gershenzon J (2009) Herbivore induction of the glucosinolate-myrosinase defense system: major trends, biochemical bases and ecological significance. Phytochem Rev 8:149–170
De Vos M, Jander G (2009) Myzus persicae (green peach aphid) salivary components induce defence responses in Arabidopsis thaliana. Plant Cell Environ 32:1548–1560
Kim JH, Jander G (2007) Myzus persicae (green peach aphid) feeding on Arabidopsis induces the formation of a deterrent indole glucosinolate. Plant J 49:1008–1019
Koritsas VM, Lewis JA, Fenwick GR (1991) Glucosinolate responses of oilseed rape, mustard and kale to mechanical wounding and infestation by cabbage stem flea beetle (Psylliodes chrysocephala). Ann Appl Biol 118:209–221
Shroff R, Vergara F, Muck A, Svatoš A, Gershenzon J (2008) Nonuniform distribution of glucosinolates in Arabidopsis thaliana leaves has important consequences for plant defense. Proc Natl Acad Sci U S A 105:6196–6201
Städler E, Renwick JAA, Radke CD, Sachdevgupta K (1995) Tarsal contact chemoreceptor response to glucosinolates and cardenolides mediating oviposition in Pieris rapae. Physiol Entomol 20:175–187
Marazzi C, Patrian B, Städler E (2004) Secondary metabolites of the leaf surface affected by sulphur fertilisation and perceived by the diamondback moth. Chemoecology 14:81–86
Marazzi C, Städler E (2004) Arabidopsis thaliana leaf-surface extracts are detected by the cabbage root fly (Delia radicum) and stimulate oviposition. Physiol Entomol 29:192–198
Renwick JA, Radke C, Sachdev-Gupta K, Städler E (1992) Leaf surface chemicals stimulating oviposition by Pieris rapae (Lepidoptera: Pieridae) on cabbage. Chemoecology 3:33–38
Badenes-Perez FR, Reichelt M, Gershenzon J, Heckel DG (2013) Interaction of glucosinolate content of Arabidopsis thaliana mutant lines and feeding and oviposition by generalist and specialist lepidopterans. Phytochemistry 86:36–43
Huang XP, Renwick JAA (1993) Differential selection of host plants by two Pieris species—the role of oviposition stimulants and deterrents. Entomol Exp Appl 68:59–69
Hicks KL (1974) Mustard oil glucosides—feeding stimulants for adult cabbage flea beetles, Phyllotreta cruciferae (Coleoptera-Chrysomelidae). Ann Entomol Soc Am 67:261–264
David WAL, Gardiner BO (1966) Mustard oil glucosides as feeding stimulants for Pieris brassicae larvae in a semi-synthetic diet. Entomol Exp Appl 9:247–255
Perkins LE, Cribb BW, Brewer PB, Hanan J, Grant M, de Torres M, Zalucki MP (2013) Generalist insects behave in a jasmonate-dependent manner on their host plants, leaving induced areas quickly and staying longer on distant parts. Proc R Soc Lond B 280:20122646
Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plantarum 97:194–208
Matile P (1980) The mustard oil bomb—compartmentation of the myrosinase system. Biochem Physiol Pflanz 175:722–731
Koroleva OA, Davies A, Deeken R, Thorpe MR, Tomos AD, Hedrich R (2000) Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk. Plant Physiol 124:599–608
Zhao ZX, Zhang W, Stanley BA, Assmann SM (2008) Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways. Plant Cell 20:3210–3226
Andreasson E, Jorgensen LB (2003) Localization of plant myrosinases and glucosinolates. Recent Adv Phytochem 37:79–99
Wittstock U, Burow M (2010) Glucosinolate breakdown in Arabidopsis: mechanism, regulation and biological significance. Arabidopsis Book 8, e0134
Wittstock U, Burow M (2007) Tipping the scales—specifier proteins in glucosinolate hydrolysis. IUBMB Life 59:744–751
Burow M, Rice M, Hause B, Gershenzon J, Wittstock U (2007) Cell- and tissue-specific localization and regulation of the epithiospecifier protein in Arabidopsis thaliana. Plant Mol Biol 64:173–185
Burow M, Bergner A, Gershenzon J, Wittstock U (2007) Glucosinolate hydrolysis in Lepidium sativum—identification of the thiocyanate-forming protein. Plant Mol Biol 63:49–61
Burow M, Losansky A, Müller R, Plock A, Kliebenstein DJ, Wittstock U (2009) The genetic basis of constitutive and herbivore-induced ESP-independent nitrile formation in Arabidopsis. Plant Physiol 149:561–574
Lambrix V, Reichelt M, Mitchell-Olds T, Kliebenstein DJ, Gershenzon J (2001) The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory. Plant Cell 13:2793–2807
Wentzell AM, Kliebenstein DJ (2008) Genotype, age, tissue, and environment regulate the structural outcome of glucosinolate activation. Plant Physiol 147:415–428
Kissen R, Bones AM (2009) Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana. J Biol Chem 284:12057–12070
Kissen R, Rossiter JT, Bones AM (2009) The ‘mustard oil bomb’: not so easy to assemble? Localization, expression and distribution of the components of the myrosinase enzyme system. Phytochem Rev 8:69–86
Tookey HL (1973) Crambe thioglucoside glucohydrolase (EC3.2.3.1)—separation of a protein required for epithiobutane formation. Can J Biochem 51:1654–1660
Mithen R, Raybould AF, Giamoustaris A (1995) Divergent selection for secondary metabolites between wild populations of Brassica oleracea and its implications for plant-herbivore interactions. Heredity 75:472–484
Mewis I, Appel HM, Hom A, Raina R, Schultz JC (2005) Major signaling pathways modulate Arabidopsis glucosinolate accumulation and response to both phloem-feeding and chewing insects. Plant Physiol 138:1149–1162
Schlaeppi K, Bodenhausen N, Buchala A, Mauch F, Reymond P (2008) The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis. Plant J 55:774–786
Blau PA, Feeny P, Contardo L, Robson DS (1978) Allylglucosinolate and herbivorous caterpillars—contrast in toxicity and tolerance. Science 200:1296–1298
Lipka V, Dittgen J, Bednarek P, Bhat R, Wiermer M, Stein M, Landtag J, Brandt W, Rosahl S, Scheel D, Llorente F, Molina A, Parker J, Sommerville S, Schulze-Lefert P (2005) Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis. Science 310:1180–1183
Lipka U, Fuchs R, Lipka V (2008) Arabidopsis non-host resistance to powdery mildews. Curr Opin Plant Biol 11:404–411
Bednarek P, Piślewska-Bednarek M, Svatoš A, Schneider B, Doubský J, Mansurova M, Humphry M, Consonni C, Panstruga R, Sanchez-Vallet A, Molina A, Schulze-Lefert P (2009) A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science 323:101–106
Tierens KFMJ, Thomma BPHJ, Brouwer M, Schmidt J, Kistner K, Porzel A, Mauch-Mani B, Cammue BPA, Broekaert WF (2001) Study of the role of antimicrobial glucosinolate-derived isothiocyanates in resistance of Arabidopsis to microbial pathogens. Plant Physiol 125:1688–1699
Li Q, Eigenbrode SD, Stringham GR, Thiagarajah MR (2000) Feeding and growth of Plutella xylostella and Spodoptera eridania on Brassica juncea with varying glucosinolate concentrations and myrosinase activities. J Chem Ecol 26:2401–2419
Lichtenstein EP, Morgan DG, Strong FM (1962) Naturally occurring insecticides—identification of 2-phenylethylisothiocyanate as an insecticide occurring naturally in edible part of turnips. J Agric Food Chem 10:30–33
Seo ST, Tang CS (1982) Hawaiian fruit-flies (Diptera: Tephritidae)—toxicity of benzyl isothiocyanate against eggs or 1st instars of three species. J Econ Entomol 75:1132–1135
Agrawal AA, Kurashige NS (2003) A role for isothiocyanates in plant resistance against the specialist herbivore Pieris rapae. J Chem Ecol 29:1403–1415
Wadleigh RW, Yu SJ (1988) Detoxification of isothiocyanate allelochemicals by glutathione transferase in three lepidopterous species. J Chem Ecol 14:1279–1288
Hanschen FS, Brüggemann N, Brodehl A, Mewis I, Schreiner M, Rohn S, Kroh LW (2012) Characterization of products from the reaction of glucosinolate-derived isothiocyanates with cysteine and lysine derivatives formed in either model systems or broccoli sprouts. J Agric Food Chem 60:7735–7745
Kawakishi S, Namiki M (1982) Oxidative cleavage of the disulfide bond of cysteine by allyl isothiocyanate. J Agric Food Chem 30:620–622
Xiao Z, Mi L, Chung FL, Veenstra TD (2012) Proteomic analysis of covalent modifications of tubulin by isothiocyanates. J Nutr 142:1377–1381
Kawakishi S, Kaneko T (1987) Interaction of proteins with allyl isothiocyanate. J Agric Food Chem 35:85–88
Cross JV, Rady JM, Foss FW, Lyons C, Macdonald TL, Templeton DJ (2009) Nutrient isothiocyanates covalently modify and inhibit the inflammatory cytokine macrophage migration inhibitory factor (MIF). Biochem J 423:315–321
Hu CQ, Eggler AL, Mesecar AD, van Breemen RB (2011) Modification of Keap1 cysteine residues by sulforaphane. Chem Res Toxicol 24:515–521
Mi L, Di Pasqua AJ, Chung FL (2011) Proteins as binding targets of isothiocyanates in cancer prevention. Carcinogenesis 32:1405–1413
Burow M, Müller R, Gershenzon J, Wittstock U (2006) Altered glucosinolate hydrolysis in genetically engineered Arabidopsis thaliana and its influence on the larval development of Spodoptera littoralis. J Chem Ecol 32:2333–2349
Mumm R, Burow M, Bukovinszkine’Kiss G, Kazantzidou E, Wittstock U, Dicke M, Gershenzon J (2008) Formation of simple nitriles upon glucosinolate hydrolysis affects direct and indirect defense against the specialist herbivore, Pieris rapae. J Chem Ecol 34:1311–1321
Kissen R, Pope TW, Grant M, Pickett JA, Rossiter JT, Powell G (2009) Modifying the alkylglucosinolate profile in Arabidopsis thaliana alters the tritrophic interaction with the herbivore Brevicoryne brassicae and parasitoid Diaeretiella rapae. J Chem Ecol 35:958–969
Pope TW, Kissen R, Grant M, Pickett JA, Rossiter JT, Powell G (2008) Comparative innate responses of the aphid parasitoid Diaeretiella rapae to alkenyl glucosinolate derived isothiocyanates, nitriles, and epithionitriles. J Chem Ecol 34:1302–1310
Bidart-Bouzat MG, Kliebenstein DJ (2008) Differential levels of insect herbivory in the field associated with genotypic variation in glucosinolates in Arabidopsis thaliana. J Chem Ecol 34:1026–1037
Lüthy J, Benn MH (1977) Thiocyanate formation from glucosinolates: a study of autolysis of allylglucosinolate in Thlaspi arvense L. seed flour extracts. Can J Biochem 55:1028–1031
Kim JH, Lee BW, Schroeder FC, Jander G (2008) Identification of indole glucosinolate breakdown products with antifeedant effects on Myzus persicae (green peach aphid). Plant J 54:1015–1026
Mauricio R (1998) Costs of resistance to natural enemies in field populations of the annual plant Arabidopsis thaliana. Am Nat 151:20–28
Siemens DH, Mitchell-Olds T (1996) Glucosinolates and herbivory by specialists (Coleoptera: Chrysomelidae, Lepidoptera: Plutellidae): consequences of concentration and induced resistance. Environ Entomol 25:1344–1353
Holzinger F, Frick C, Wink M (1992) Molecular basis for the insensitivity of the monarch (Danaus plexippus) to cardiac glycosides. FEBS Lett 314:477–480
Self LS, Hodgson E, Guthrie FE (1964) Metabolism of nicotine by tobacco-feeding insects. Nature 204:300–301
Ivie GW, Bull DL, Beier RC, Pryor NW, Oertli EH (1983) Metabolic detoxification: mechanism of insect resistance to plant psoralens. Science 221:374–376
Hartmann T (1999) Chemical ecology of pyrrolizidine alkaloids. Planta 207:483–495
Dussourd DE, Eisner T (1987) Vein-cutting behavior: insect counterploy to the latex defense of plants. Science 237:898–901
Yu SJ (1984) Interactions of allelochemicals with detoxication enzymes of insecticide-susceptible and resistant fall armyworms. Pest Biochem Physiol 22:60–68
Iqbal M, Wright DJ (1997) Evaluation of resistance, cross-resistance and synergism of abamectin and teflubenzuron in a multi-resistant field population of Plutella xylostella (Lepidoptera: Plutellidae). Bull Entomol Res 87:481–486
Furlong MJ, Wright DJ (1994) Examination of stability of resistance and cross-resistance patterns to acylurea insect growth-regulators in-field populations of the diamondback moth, Plutella xylostella, from Malaysia. Pestic Sci 42:315–326
Bartlet E, Parsons D, Williams IH, Clark SJ (1994) The influence of glucosinolates and sugars on feeding by the cabbage stem flea beetle, Psylliodes chrysocephala. Entomol Exp Appl 73:77–83
Nault LR, Styer WE (1972) Effects of sinigrin on host selection by aphids. Entomol Exp Appl 15:423–437
Lankau RA (2007) Specialist and generalist herbivores exert opposing selection on a chemical defense. New Phytol 175:176–184
Nishida R (2002) Sequestration of defensive metabolites from plants by lepidoptera. Annu Rev Entomol 47:57–92
Francis F, Lognay G, Wathelet JP, Haubruge E (2001) Effects of allelochemicals from first (Brassicaceae) and second (Myzus persicae and Brevicoryne brassicae) trophic levels on Adalia bipunctata. J Chem Ecol 27:243–256
Müller C, Agerbirk N, Olsen CE, Boevé JL, Schaffner U, Brakefield PM (2001) Sequestration of host plant glucosinolates in the defensive hemolymph of the sawfly Athalia rosae. J Chem Ecol 27:2505–2516
Aliabadi A, Renwick JAA, Whitman DW (2002) Sequestration of glucosinolates by harlequin bug Murgantia histrionica. J Chem Ecol 28:1749–1762
Winde I, Wittstock U (2011) Insect herbivore counteradaptations to the plant glucosinolate-myrosinase system. Phytochemistry 72:1566–1575
Giamoustaris A, Mithen R (1995) The effect of modifying the glucosinolate content of leaves of oilseed rape (Brassica napus ssp. oleifera) on its interaction with specialist and generalist pests. Ann Appl Biol 126:347–363
Hilker M, Meiners T (2002) Induction of plant responses to oviposition and feeding by herbivorous arthropods: a comparison. Entomol Exp Appl 104:181–192
Isidoro N, Bartlet E, Ziesmann J, Williams IH (1998) Antennal contact chemosensilla in Psylliodes chrysocephala responding to cruciferous allelochemicals. Physiol Entomol 23:131–138
Roessingh P, Städler E, Baur R, Hurter J, Ramp T (1997) Tarsal chemoreceptors and oviposition behaviour of the cabbage root fly (Delia radicum) sensitive to fractions and new compounds of host-leaf surface extracts. Physiol Entomol 22:140–148
Nielsen JK, Hansen ML, Agerbirk N, Petersen BL, Halkier BA (2001) Responses of the flea beetles Phyllotreta nemorum and P. cruciferae to metabolically engineered Arabidopsis thaliana with an altered glucosinolate profile. Chemoecology 11:75–83
Sarfraz M, Dosdall LM, Keddie BA (2006) Diamondback moth-host plant interactions: implications for pest management. Crop Prot 25:625–639
Åhman I (1986) Toxicities of host secondary compounds to eggs of the Brassica specialist Dasineura brassicae. J Chem Ecol 12:1481–1488
Wittstock U, Agerbirk N, Stauber EJ, Olsen CE, Hippler M, Mitchell-Olds T, Gershenzon J, Vogel H (2004) Successful herbivore attack due to metabolic diversion of a plant chemical defense. Proc Natl Acad Sci U S A 101:4859–4864
Ratzka A, Vogel H, Kliebenstein DJ, Mitchell-Olds T, Kroymann J (2002) Disarming the mustard oil bomb. Proc Natl Acad Sci U S A 99:11223–11228
Wheat CW, Vogel H, Wittstock U, Braby MF, Underwood D, Mitchell-Olds T (2007) The genetic basis of a plant-insect coevolutionary key innovation. Proc Natl Acad Sci U S A 104:20427–20431
Falk KL, Gershenzon J (2007) The desert locust, Schistocerca gregaria, detoxifies the glucosinolates of Schouwia purpurea by desulfation. J Chem Ecol 33:1542–1555
Ehrlich PR, Raven PH (1964) Butterflies and plants—a study in coevolution. Evolution 18:586–608
Beilstein MA, Nagalingum NS, Clements MD, Manchester SR, Mathews S (2010) Dated molecular phylogenies indicate a Miocene origin for Arabidopsis thaliana. Proc Natl Acad Sci U S A 107:18724–18728
Vergara F, Svatoš A, Schneider B, Reichelt M, Gershenzon J, Wittstock U (2006) Glycine conjugates in a lepidopteran insect herbivore—the metabolism of benzylglucosinolate in the cabbage white butterfly, Pieris rapae. Chembiochem 7:1982–1989
Agerbirk N, Olsen CE, Topbjerg HB, Sørensen JC (2007) Host plant-dependent metabolism of 4-hydroxybenzylglucosinolate in Pieris rapae: Substrate specificity and effects of genetic modification and plant nitrile hydratase. Insect Biochem Mol Biol 37:1119–1130
Agerbirk N, Olsen CE, Poulsen E, Jacobsen N, Hansen PR (2010) Complex metabolism of aromatic glucosinolates in Pieris rapae caterpillars involving nitrile formation, hydroxylation, demethylation, sulfation, and host plant dependent carboxylic acid formation. Insect Biochem Mol Biol 40:126–137
Agerbirk N, Müller C, Olsen CE, Chew FS (2006) A common pathway for metabolism of 4-hydroxybenzylglucosinolate in Pieris and Anthocaris (Lepidoptera: Pieridae). Biochem Syst Ecol 34:189–198
Winde IB (2011) Entgiftung des Glucosinolat-Myrosinase-systems durch generalistische Herbivoren der Lepidoptera. Doctoral Thesis, Technical University Braunschweig
Stauber EJ, Kuczka P, van Ohlen M, Vogt B, Janowitz T, Piotrowski M, Beuerle T, Wittstock U (2012) Turning the ‘mustard oil bomb’ into a ‘cyanide bomb’: aromatic glucosinolate metabolism in a specialist insect herbivore. PLoS One 7, e35545
Ballhorn DJ, Kautz S, Heil M, Hegeman A (2009) Cyanogenesis of wild lima bean (Phaseolus lunatus L.) is an efficient direct defence in nature. PLoS One 4, e5450
Gleadow RM, Woodrow IE (2002) Constraints on effectiveness of cyanogenic glycosides in herbivore defense. J Chem Ecol 28:1301–1313
Conn EE (1980) Cyanogenic compounds. Annu Rev Plant Phys 31:433–451
Petroski RJ, Kwolek WF (1985) Interactions of a fungal thioglucoside glucohydrolase and cruciferous plant epithiospecifier protein to form 1-cyanoepithioalkanes: implications of an allosteric mechanism. Phytochemistry 24:213–216
Foo HL, Grønning LM, Goodenough L, Bones AM, Danielsen BE, Whiting DA, Rossiter JT (2000) Purification and characterisation of epithiospecifier protein from Brassica napus: Enzymic intramolecular sulphur addition within alkenyl thiohydroximates derived from alkenyl glucosinolate hydrolysis. FEBS Lett 468:243–246
Burow M, Markert J, Gershenzon J (2006) Comparative biochemical characterization of nitrile-forming proteins from plants and insects that alter myrosinase-catalysed hydrolysis of glucosinolates. FEBS J 273:2432–2446
Rothschild M, Schoonhoven LM (1977) Assessment of egg load by Pieris brassicae (Lepidoptera: Pieridae). Nature 266:352–355
Mewis I, Tokuhisa JG, Schultz JC, Appel HM, Ulrichs C, Gershenzon J (2006) Gene expression and glucosinolate accumulation in Arabidopsis thaliana in response to generalist and specialist herbivores of different feeding guilds and the role of defense signaling pathways. Phytochemistry 67:2450–2462
Barth C, Jander G (2006) Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense. Plant J 46:549–562
Fatouros NE, Broekgaarden C, Bukovinszkine’Kiss G, van Loon JJA, Mumm R, Huigens ME, Dicke M, Hilker M (2008) Male-derived butterfly anti-aphrodisiac mediates induced indirect plant defense. Proc Natl Acad Sci U S A 105:10033–10038
Fatouros NE, Huigens ME, van Loon JJA, Dicke M, Hilker M (2005) Chemical communication—butterfly anti-aphrodisiac lures parasitic wasps. Nature 433:704
Andersson J, Borg-Karlson AK, Wiklund C (2003) Antiaphrodisiacs in pierid butterflies: a theme with variation! J Chem Ecol 29:1489–1499
Thies W (1979) Detection and utilization of a glucosinolate sulfohydrolase in the edible snail, Helix pomatia. Naturwissenschaften 66:364–365
Shikita M, Fahey JW, Golden TR, Holtzclaw WD, Talalay P (1999) An unusual case of ‘uncompetitive activation’ by ascorbic acid: purification and kinetic properties of a myrosinase from Raphanus sativus seedlings. Biochem J 341:725–732
Sarosh BR, Wittstock U, Halkier BA, Ekbom B (2010) The influence of metabolically engineered glucosinolates profiles in Arabidopsis thaliana on Plutella xylostella preference and performance. Chemoecology 20:1–9
Kliebenstein D, Pedersen D, Barker B, Mitchell-Olds T (2002) Comparative analysis of quantitative trait loci controlling glucosinolates, myrosinase and insect resistance in Arabidopsis thaliana. Genetics 161:325–332
Falk KL, Kästner J, Bodenhausen N, Schramm K, Paetz C, Vassão DG, Reichelt M, von Knorre D, Bergelson J, Erb M, Gershenzon J, Meldau S (2014) The role of glucosinolates and the jasmonic acid pathway in resistance of Arabidopsis thaliana against molluscan herbivores. Mol Ecol 23:1188–1203
Ghaout S, Louveaux A, Mainguet AM, Deschamps M, Rahal Y (1991) What defense does Schouwia purpurea (Cruciferae) have against the desert locust—secondary compounds and nutritive value. J Chem Ecol 17:1499–1515
Mainguet AM, Louveaux A, El Sayed G, Rollin P (2000) Ability of a generalist insect, Schistocerca gregaria, to overcome thioglucoside defense in desert plants: Tolerance or adaptation? Entomol Exp Appl 94:309–317
Terriere LC (1984) Induction of detoxification enzymes in insects. Annu Rev Entomol 29:71–88
Yu SJ, Hsu EL (1993) Induction of detoxification enzymes in phytophagous insects: roles of insecticide synergists, larval age, and species. Arch Insect Biochem Physiol 24:21–32
Hoy CW, Head GP, Hall FR (1998) Spatial heterogeneity and insect adaption to toxins. Annu Rev Entomol 43:571–594
Noctor G, Queval G, Mhamdi A, Chaouch S, Foyer CH (2011) Glutathione. Arabidopsis Book 9, e0142
Gloss AD, Vassão DG, Hailey AL, Dittrich ACN, Schramm K, Reichelt M, Rast TJ, Weichsel A, Cravens MG, Gershenzon J, Montfort WR, Whiteman NK (2014) Evolution in an ancient detoxification pathway is coupled with a transition to herbivory in the Drosophilidae. Mol Biol Evol 31:2441–2456
Al Janobi AA, Mithen RF, Gasper AV, Shaw PN, Middleton RJ, Ortori CA, Barrett DA (2006) Quantitative measurement of sulforaphane, iberin and their mercapturic acid pathway metabolites in human plasma and urine using liquid chromatography-tandem electrospray ionisation mass spectrometry. J Chromatogr B 844:223–234
Kassahun K, Davis M, Hu P, Martin B, Baillie T (1997) Biotransformation of the naturally occurring isothiocyanate sulforaphane in the rat: Identification of phase I metabolites and glutathione conjugates. Chem Res Toxicol 10:1228–1233
Eklind KI, Morse MA, Chung FL (1990) Distribution and metabolism of the natural anticarcinogen phenethyl isothiocyanate in A/J mice. Carcinogenesis 11:2033–2036
Francis F, Vanhaelen N, Haubruge E (2005) Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid. Arch Insect Biochem Physiol 58:166–174
Tjallingii WF, Hogen Esch T (1993) Fine structure of aphid stylet routes in plant-tissues in correlation with EPG signals. Physiol Entomol 18:317–328
Husebye H, Chadchawan S, Winge P, Thangstad OP, Bones AM (2002) Guard cell- and phloem idioblast-specific expression of thioglucoside glucohydrolase 1 (myrosinase) in Arabidopsis. Plant Physiol 128:1180–1188
Thangstad OP, Gilde B, Chadchawan S, Seem M, Husebye H, Bradley D, Bones AM (2004) Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum. Plant Mol Biol 54:597–611
Pedras MS, Nycholat CM, Montaut S, Xu Y, Khan AQ (2002) Chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip. Phytochemistry 59:611–625
Bodnaryk RP (1994) Potent effect of jasmonates on indole glucosinolates in oilseed rape and mustard. Phytochemistry 35:301–305
Ramsey JS, Wilson ACC, de Vos M, Sun Q, Tamborindeguy C, Winfield A, Malloch G, Smith DM, Fenton B, Gray SM, Jander G (2007) Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design. BMC Genomics 8:423
Agerbirk N, Olsen CE, Sørensen H (1998) Initial and final products, nitriles, and ascorbigens produced in myrosinase-catalyzed hydrolysis of indole glucosinolates. J Agric Food Chem 46:1563–1571
Pfalz M, Vogel H, Kroymann J (2009) The gene controlling the indole glucosinolate modifier1 quantitative trait locus alters indole glucosinolate structures and aphid resistance in Arabidopsis. Plant Cell 21:985–999
Opitz S, Müller C (2009) Plant chemistry and insect sequestration. Chemoecology 19:117–154
Duffey SS (1980) Sequestration of plant natural-products by insects. Annu Rev Entomol 25:447–477
Abe F, Yamauchi T, Honda K, Omura H, Hayashi N (2001) Sequestration of phenanthroindolizidine alkaloids by an Asclepiadaceae-feeding danaid butterfly, Ideopsis similis. Phytochemistry 56:697–701
Dobler S, Daloze D, Pasteels JM (1998) Sequestration of plant compounds in a leaf beetle’s defensive secretion: cardenolides in Chrysochus. Chemoecology 8:111–118
Dobler S, Haberer W, Witte L, Hartmann T (2000) Selective sequestration of pyrrolizidine alkaloids from diverse host plants by Longitarsus flea beetles. J Chem Ecol 26:1281–1298
Schittko U, Burghardt F, Fiedler K (1999) Sequestration and distribution of flavonoids in the common blue butterfly Polyommatus icarus reared on Trifolium repens. Phytochemistry 51:609–614
Scudder GGE, Moore LV, Isman MB (1986) Sequestration of cardenolides in Oncopeltus fasciatus: morphological and physiological adaptations. J Chem Ecol 12:1171–1187
Rothschild M, Edgar JA (1978) Pyrrolizidine alkaloids from Senecio vulgaris sequestered and stored by Danaus plexippus. J Zool 186:347–349
Trigo JR (2000) The chemistry of antipredator defense by secondary compounds in neotropical lepidoptera: facts, perspectives and caveats. J Brazil Chem Soc 11:551–561
Francis F, Lognay G, Wathelet JP, Haubruge E (2002) Characterisation of aphid myrosinase and degradation studies of glucosinolates. Arch Insect Biochem Physiol 50:173–182
Müller C (2009) Interactions between glucosinolate- and myrosinase-containing plants and the sawfly Athalia rosae. Phytochem Rev 8:121–134
Francis F, Haubruge E, Gaspar C (2000) Influence of host plants on specialist/generalist aphids and on the development of Adalia bipunctata (Coleoptera: Coccinellidae). Eur J Entomol 97:481–485
Bridges M, Jones AME, Bones AM, Hodgson C, Cole R, Bartlet E, Wallsgrove R, Karapapa VK, Watts N, Rossiter JT (2002) Spatial organization of the glucosinolate-myrosinase system in brassica specialist aphids is similar to that of the host plant. Proc R Soc Lond B 269:187–191
Müller C, Brakefield PM (2003) Analysis of a chemical defense in sawfly larvae: easy bleeding targets predatory wasps in late summer. J Chem Ecol 29:2683–2694
Boevé JL, Schaffner U (2003) Why does the larval integument of some sawfly species disrupt so easily? The harmful hemolymph hypothesis. Oecologia 134:104–111
Ohara Y, Nagasaka K, Ohsaki N (1993) Warning coloration in sawfly Athalia rosae larva and concealing coloration in butterfly Pieris rapae larva feeding on similar plants evolved through individual selection. Res Popul Ecol 35:223–230
Schaffner U, Boevé JL, Gfeller H, Schlunegger UP (1994) Sequestration of Veratrum alkaloids by specialist Rhadinoceraea nodicornis Konow (Hymenoptera, Tenthredinidae) and its ecoethological implications. J Chem Ecol 20:3233–3250
Heads PA, Lawton JH (1985) Bracken, ants and extrafloral nectaries. III. How insect herbivores avoid ant predation. Ecol Entomol 10:29–42
Müller C, Wittstock U (2005) Uptake and turn-over of glucosinolates sequestered in the sawfly Athalia rosae. Insect Biochem Mol Biol 35:1189–1198
Müller C, Zwaan BJ, de Vos H, Brakefield PM (2003) Chemical defence in a sawfly: genetic components of variation in relevant life-history traits. Heredity 90:468–475
Opitz SEW, Mix A, Winde IB, Müller C (2011) Desulfation followed by sulfation: metabolism of benzylglucosinolate in Athalia rosae (Hymenoptera: Tenthredinidae). Chembiochem 12:1252
Abdalsamee MK, Giampà M, Niehaus K, Müller C (2014) Rapid incorporation of glucosinolates as a strategy used by a herbivore to prevent activation by myrosinases. Insect Biochem Mol Biol 52:115–123
Yang RSH, Wilkinson CF (1973) Sulfotransferases and phosphotransferases in insects. Comp Biochem Physiol 46:717–726
Smith JN (1955) Comparative detoxication. 4. Ethereal sulphate and glucoside conjugations in insects. Biochem J 60:436–442
Homolya L, Váradi A, Sarkadi B (2003) Multidrug resistance-associated proteins: export pumps for conjugates with glutathione, glucuronate or sulfate. Biofactors 17:103–114
Liu S, Zhou S, Tian L, Guo E, Luan Y, Zhang J, Li S (2011) Genome-wide identification and characterization of ATP-binding cassette transporters in the silkworm, Bombyx mori. BMC Genomics 12:491
Discher S, Burse A, Tolzin-Banasch K, Heinemann SH, Pasteels JM, Boland W (2009) A versatile transport network for sequestering and excreting plant glycosides in leaf beetles provides an evolutionary flexible defense strategy. Chembiochem 10:2223–2229
Kuhn J, Pettersson EM, Feld BK, Burse A, Termonia A, Pasteels JM, Boland W (2004) Selective transport systems mediate sequestration of plant glucosides in leaf beetles: a molecular basis for adaptation and evolution. Proc Natl Acad Sci U S A 101:13808–13813
Strauss AS, Peters S, Boland W, Burse A (2013) ABC transporter functions as a pacemaker for sequestration of plant glucosides in leaf beetles. eLife 2, e01096
Kazana E, Pope TW, Tibbles L, Bridges M, Pickett JA, Bones AM, Powell G, Rossiter JT (2007) The cabbage aphid: a walking mustard oil bomb. Proc R Soc Lond B 274:2271–2277
Vanhaelen N, Haubruge E, Lognay G, Francis F (2001) Hoverfly glutathione S-transferases and effect of Brassicaceae secondary metabolites. Pest Biochem Physiol 71:170–177
Pratt C, Pope TW, Powell G, Rossiter JT (2008) Accumulation of glucosinolates by the cabbage aphid Brevicoryne brassicae as a defense against two coccinellid species. J Chem Ecol 34:323–329
Chaplin-Kramer R, Kliebenstein DJ, Chiem A, Morrill E, Mills NJ, Kremen C (2011) Chemically mediated tritrophic interactions: opposing effects of glucosinolates on a specialist herbivore and its predators. J Appl Ecol 48:880–887
Bayhan SÖ, Ulusoy MR, Bayhan E (2007) Is the parasitization rate of Diaeretiella rapae influenced when Brevicoryne brassicae feeds on Brassica plants? Phytoparasitica 35:146–149
Husebye H, Arzt S, Burmeister WP, Härtel FV, Brandt A, Rossiter JT, Bones AM (2005) Crystal structure at 1.1Å resolution of an insect myrosinase from Brevicoryne brassicae shows its close relationship to ß-glucosidases. Insect Biochem Mol Biol 35:1311–1320
Jones AME, Bridges M, Bones AM, Cole R, Rossiter JT (2001) Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.). Insect Biochem Mol Biol 31:1–5
Pontoppidan B, Ekbom B, Eriksson S, Meijer J (2001) Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore. Eur J Biochem 268:1041–1048
Agerbirk N, Vos M, Kim JH, Jander G (2008) Indole glucosinolate breakdown and its biological effects. Phytochem Rev 8:101–120
Aldrich JR, Avery JW, Lee CJ, Graf JC, Harrisons DJ, Bin F (1996) Semiochemistry of cabbage bugs (Heteroptera: Pentatomidae: Eurydema and Murgantia). J Entomol Sci 31:172–182
Dawson GW, Griffiths DC, Pickett JA, Wadhams LJ, Woodcock CM (1987) Plant-derived synergists of alarm pheromone from turnip aphid, Lipaphis (Hyadaphis) erysimi (Homoptera, Aphididae). J Chem Ecol 13:1663–1671
Beran F, Mewis I, Srinivasan R, Svoboda J, Vial C, Mosimann H, Boland W, Büttner C, Ulrichs C, Hansson BS, Reinecke A (2011) Male Phyllotreta striolata (F.) produce an aggregation pheromone: identification of male-specific compounds and interaction with host plant volatiles. J Chem Ecol 37:85–97
Beran F, Pauchet Y, Kunert G, Reichelt M, Wielsch N, Vogel H, Reinecke A, Svatoš A, Mewis I, Schmid D, Ramasamy S, Ulrichs C, Hansson BS, Gershenzon J, Heckel DG (2014) Phyllotreta striolata flea beetles use host plant defense compounds to create their own glucosinolate-myrosinase system. Proc Natl Acad Sci U S A 111:7349–7354
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Jeschke, V., Gershenzon, J., Vassão, D.G. (2015). Metabolism of Glucosinolates and Their Hydrolysis Products in Insect Herbivores. In: Jetter, R. (eds) The Formation, Structure and Activity of Phytochemicals. Recent Advances in Phytochemistry, vol 45. Springer, Cham. https://doi.org/10.1007/978-3-319-20397-3_7
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