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Industrial Monitoring of Cell Culture

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Cell Culture Engineering and Technology

Part of the book series: Cell Engineering ((CEEN,volume 10))

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Abstract

The monitoring of cell cultivation processes is at the core of every such cultivation. It is the basis for thoroughly understanding each cell line’s growth and metabolism characteristics and to ensure reproducible cell cultivation processes. Monitoring comprises physiological and biological quality attributes like cell count and viability, substrate, metabolite and product concentrations, as well as a large set of physicochemical parameters. While many publications on this topic describe in detail the large number of different monitoring concepts, techniques and developments, only a few look at it from the perspective of an industrial application: Which monitoring techniques are used in process development, which in large-scale manufacturing? How do regulatory requirements impact the implementation of monitoring devices? Here, we concentrate on the subset of monitoring techniques – old and new – that are used in an industrial cell cultivation context or, in the opinion of the authors, have the potential to make it in the near future.

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Acknowledgements

The authors are indebted to Andrea McIntosh-Suhr and Tanja Bergfeld for critical comments on this manuscript.

Disclosure of interests

All authors declare they have no conflict of interest.

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Authors and Affiliations

  1. Rentschler Biopharma SE, Process Science, Laupheim, Germany

    Sebastian Schwamb

  2. Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany

    Markus Engel

  3. Department of Biotechnology, Mannheim University of Applied Sciences, Mannheim, Germany

    Tobias Werner & Philipp Wiedemann

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Correspondence to Philipp Wiedemann .

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  1. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany

    Ralf Pörtner

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Schwamb, S., Engel, M., Werner, T., Wiedemann, P. (2021). Industrial Monitoring of Cell Culture. In: Pörtner, R. (eds) Cell Culture Engineering and Technology. Cell Engineering, vol 10. Springer, Cham. https://doi.org/10.1007/978-3-030-79871-0_17

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