Abstract
Blast is one of the devastating diseases of rice which cause significant yield losses. Blast is mainly managed by the use of fungicides. However, this approach is not eco-friendly and causes health hazards. Basmati is the specialty rice of India with great export potential. However, the importing nations have increased the stringency in maximum permissible limits of pesticide residues in the grain, which has led to rejection of several Basmati rice consignments. Therefore, developing genetic resistance is one of the most pragmatic approaches to address this issue. More than 100 blast resistance genes have been identified which can be effectively deployed into the high-yielding rice varieties through marker-assisted backcross breeding. In India the major blast resistance genes, namely Pi9, Pi2, Pi54, Pita, and Pi1, are widely used to develop blast resistance in popular rice varieties. Until recently, blast-resistant varieties developed in India are Pusa Basmati 1637, Pusa Basmati 1609, Pusa 1612, and Pusa Samba 1850. Effective adoption of these varieties would significantly reduce the use of chemical pesticides, thereby producing consumer-safe rice with eco-friendly approach.
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References
Ashikawa I, Hayashi N, Yamane H, Kanamori H, Wu J, Matsumoto T, Ono K, Yano M. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics. 2008;180:2267–76.
Ashkani S, Yusop MR, Shabanimofrad M, Azadi A, Ghasemzadeh A, Azizi P, Latif MA. Allele mining strategies: principles and utilisation for blast resistance genes in rice (Oryza sativa L.). Curr Issues Mol Biol. 2015;17:57–74.
Böhnert HU, Fudal I, Dioh W, Tharreau D, Notteghem JL, Lebrun MH. A putative polyketide synthase/peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice. Plant Cell. 2004;16:2499–513.
Braun EJ, Howard RJ. Adhesion of fungal spores and germlings to host plant surfaces. Protoplasma. 1994;181(1–4):202–12.
Bryan GT, Wu KS, Farrall L, Jia Y, Hershey HP, McAdams SA, Faulk KN, Donaldson GK, Tarchini R, Valent B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell. 2000;12:2033–46.
Cesari S, Thilliez G, Ribot C, Chalvon V, Michel C, Jauneau A, Rivas S, Alaux L, Kanzaki H, Okuyama Y, Fournier EJ, Tharreau D, Terauchi R, Kroja T. The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell. 2013;25:1463–81.
Chen X, Shang J, Chen D, Lei C, Zou Y, Zhai W, Liu G, Xu J, Ling Z, Cao G, Ma B, Wang Y, Zhao X, Li S, Zhu L. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J. 2006;46:794–804.
Chen J, Shi YF, Liu W, Chai R, Fu Y, Zhuang J, Wu J. A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae. J Genet Genomics. 2011;38:209–16.
Chen J, Peng P, Tian J, He Y, Zhang L, Liu Z, Yin D, Zhang Z. Pike, a rice blast resistance allele consisting of two adjacent NBS-LRR genes, was identified as a novel allele at the Pik locus. Mol Breed. 2015;35:117.
Collard BCY, Mackill DJ. Marker-assisted selection: an approach for precision plant breeding in the 21st century. Philos Trans R Soc B Rev. 2008;363:557–72.
Couch BC, Kohn LM. A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. Mycologia. 2002;94:683–93.
Dangl JL, Horvath DM, Staskawicz BJ. Pivoting the plant immune system from dissection to deployment. Science. 2013;341:746–51.
Das A, Soubam D, Singh PK, Thakur S, Singh NK, Sharma TR. A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae. Funct Integr Genomics. 2012;12:215–28.
Deng Y, Zhai K, Xie Z, Yang D, Zhu X, Liu J, Wang X, Qin P, Yang Y, Zhang G, Li Q, Zhang J, Wu S, Milazzo J, Mao B, Wang E, Xie H, Tharreau D, He Z. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance. Science. 2017;355:962–5.
Devanna NB, Vijayan J, Sharma TR. The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains. PLoS One. 2014;9:e104840.
Dong TG, Ho BT, Yoder-Himes DR, Mekalanos JJ. Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholera. Proc Natl Acad Sci U S A. 2013;110(7):2623–8.
Ellur RK, Khanna A, Yadav A, Pathania S, Rajashekara H, Singh VK, Krishnan SG, Bhowmick PK, Nagarajan M, Vinod KK, Prakash G. Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding. Plant Sci. 2016;242:330–41.
Feng S, Wang L, Ma J, Lin F, Pan Q. Genetic and physical mapping of AvrPi7, a novel avirulence gene of Magnaporthe oryzae using physical position-ready markers. Chin Sci Bull. 2007;52:903–11.
Fitzgerald MA, Sackville Hamilton NR, Calingacion MN, Verhoeven HA, Butardo VM. Is there a second fragrance gene in rice? Plant Biotechnol J. 2008;6:416–23.
Flor HH. The complementary genetic systems in flax rust. Adv Genet. 1956;8(29):54.
Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K. Loss of function of a proline-containing protein confers durable disease resistance in rice. Science. 2009;325:998–1001.
Fukuoka S, Yamamoto SI, Mizobuchi R, Yamanouchi U, Ono K, Kitazawa N, Yasuda N, Fujita Y, Nguyen TT, Koizumi S, Sugimoto K, Matsumoto T, Yano M. Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast. Sci Rep. 2014;4:1–7.
Fukuta Y, Araki E, Telebanco-Yanoria MJ, Ebron L, Mercado-Escueta D, Takai T, Khush GS. Identification of blast resistance gene, Pish in rice (Oryza sativa L.). In: Proc. Plant and Animal Genome XII Conference, San Diego, CA, 10–14 January. 2004. p. 401.
Ghatak A, Willocquet L, Savary S, Kumar J. Variability in aggressiveness of rice blast (Magnaporthe oryzae) isolates originating from rice leaves and necks: a case of pathogen specialization. PLoS One. 2013;8(6):e66180.
Hayashi K, Yoshida H. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter. Plant J. 2009;57:413–25.
Hayashi N, Inoue H, Kato T, Funao T, Shirota M, Shimizu T, Kanamori H, Yamane H, Hayano-Saito Y, Matsumoto T. Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication. Plant J. 2010;64:498–510.
Hittalmani S, Parco A, Mew TV, Zeigler RS, Huang N. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor Appl Genet. 2000;100(7):1121–8.
Hospital F, Charcosset A. Marker assisted introgression of quantitative trait loci. Genetics. 1997;147:1469–85.
Howard RJ, Bourett TM, Ferrari MA. In: Mendgen K, Lesemann DE, editors. Infection by Magnaporthe grisea: an in vitro analysis. Berlin: Springer; 1991a. p. 251–64.
Howard RJ, Ferrari MA, Roach DH, Money NP. Penetration of hard substrates by a fungus employing enormous turgor pressures. Proc Natl Acad Sci. 1991b;88:11281–4.
Hua L, Wu J, Chen C, Wu W, He X, Lin F, Wang L, Ashikawa I, Matsumoto T, Wang L, Pan Q. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast. Theor Appl Genet. 2012;125:1047–55.
Jain P, Singh PK, Kapoor R, Khanna A, Solanke AU, Krishnan SG, Singh AK, Sharma V, Sharma TR. Understanding host-pathogen interactions with expression profiling of NILs carrying rice-blast resistance Pi9 gene. Front Plant Sci. 2017;8:9.
Khan GH, Shikari AB, Vaishnavi R, Najeeb S, Padder BA, Bhat ZA, Parray GA, Bhat MA, Kumar R, Singh NK. Marker-assisted introgression of three dominant blast resistance genes into an aromatic rice cultivar Mushk Budji. Sci Rep. 2018;8(1):1–3.
Khanna A, Sharma V, Ellur RK, Shikari AB, Gopala Krishnan S, Singh UD, Prakash G, Sharma TR, Rathour R, Variar M, Prashanthi SK, Nagarajan M, Vinod KK, Bhowmick PK, Singh NK, Prabhu KV, Singh BD, Singh AK. Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice. Theor Appl Genet. 2015a;128(7):1243–59.
Khanna A, Sharma V, Ellur RK, Shikari AB, Krishnan SG, Singh UD, Prakash G, Sharma TR, Rathour R, Variar M, Prashanthi SK. Marker assisted pyramiding of major blast resistance genes Pi9 and Pita in the genetic background of an elite Basmati rice variety, Pusa Basmati 1. Indian J Genet. 2015b;75(4):417–25.
Kumar MKP, Gowda DKS, Moudgal R, Kumar NK, Gowda KTP, Vishwanath K. Impact on fungicides on rice production in India. In: Nita M, editor. Fungicides-showcases of integrated plant disease management from around the world. InTech; 2013. ISBN: 978-953-51-1130-6. https://doi.org/10.5772/51009. http://www.intechopen.com/books/fungicides-showcases-of-integrated-plant-disease-managementfrom-around-the-world/impact-of-fungicides-on-rice-production-in-india. Accessed 18 Aug 2014.
Lee S, Song M, Seo Y, Kim S, Ko S, Cao P, Suh J, Yi G, Roh J, Lee S, An G, Hahn TR, Wang GL, Ronald P, Jeon JS. Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes. Genetics. 2009;181:1627–38.
Li W, Wang B, Wu J, Lu G, Hu Y, Zhang X, Zhang Z, Zhao Q, Feng Q, Zhang H, Wang Z, Wang G, Han B, Wang Z, Zhou B. The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t. Mol Plant Microbe Interact. 2009;22:411–20.
Lin F, Chen S, Que Z, Wang L, Liu X, Pan Q. The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1. Genetics. 2007;177:1871–80.
Liu X, Lin F, Wang L, Pan Q. The in silico map-based cloning of Pi36, a rice coiled-coil-nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus. Genetics. 2007;176:2541–9.
Liu Y, Liu B, Zhu X, Yang J, Bordeos A, Wang G, Leach JE, Leung H. Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice. Theor Appl Genet. 2013;126:985–98.
Lü Q, Xu X, Shang J, Jiang G, Pang Z, Zhou Z, Wang J, Liu Y, Li T, Li X, Xu J, Cheng Z, Zhao X, Li S, Zhu L. Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice. Phytopathology. 2013;103:594–9.
Ma J, Lei C, Xu X, Hao K, Wang J, Cheng Z, Ma X, Ma J, Zhou K, Zhang X, Guo X, Wu F, Lin Q, Wang C, Zhai H, Wang H, Wan J. Pi64, encoding a novel CC-NBS-LRR protein, confers resistance to leaf and neck blast in rice. Mol Plant-Microbe Interact. 2015;28:558–68.
Meng X, Xiao G, Telebanco-Yanoria MJ, Siazon PM, Padilla J, Opulencia R, Bigirimana J, Habarugira G, Wu J, Li M, Wang B. The broad-spectrum rice blast resistance (R) gene Pita2 encodes a novel R protein unique from Pita. Rice. 2020;13(1):1–5.
Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, Fujibe T, Matsumura H, Shenton M, Galam DC, Undan J, Ito A, Sone T, Terauchi R. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J. 2011;66:467–79.
Orbach MJ, Farrall L, Sweigard JA, Chumley FG, Valent B. A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta. Plant Cell. 2000;12:2019–32.
Park JY, Jin J, Lee YW, Kang S, Lee YH. Rice blast fungus (Magnaporthe oryzae) infects Arabidopsis via a mechanism distinct from that required for the infection of rice. Plant Physiol. 2009;149:474–86.
Qu SH, Liu GF, Zhou B, Bellizzi M, Zeng LR, Dai LY, Han B, Wang GL. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics. 2006;172:1901–14.
Ray S, Singh PK, Gupta DK, Mahato AK, Sarkar C, Rathour R, Singh NK, Sharma TR. Analysis of Magnaporthe oryzae genome reveals a fungal effector, which is able to induce resistance response in transgenic rice line containing resistance gene, Pi54. Front Plant Sci. 2016;7:1140.
Schulze-Lefert P, Panstruga R. A molecular evolutionary concept connecting nonhost resistance, pathogen host range, and pathogen speciation. Trends Plant Sci. 2011;16:117–25.
Seck PA, Diagne A, Mohanty S, Wopereis MCS. Crops that feed the world. Food Sec. 2012;4:7–24.
Shang J, Tao Y, Chen X, Zou Y, Lei C, Wang J, Li X, Zhao X, Zhang M, Lu Z, Xu J, Cheng Z, Wan J, Zhu L. Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes. Genetics. 2009;182:1303–11.
Sharma TR, Madhav MS, Singh BK, Shanker P, Jana TK, Dalal V, Pandit A, Singh A, Gaikwad K, Upreti HC, Singh NK. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea. Mol Gen Genomics. 2005;274:569–78.
Sharma TR, Rai AK, Gupta SK, Singh NK. Broad-spectrum blast resistance gene Pi-kh cloned from rice line Tetep designated as Pi54. J Plant Biochem Biotechnol. 2010;19:87–9.
Silue D, Notteghem JL, Tharreau D. Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe oryzae pathosystem. Phytopathology. 1992;82:577–80.
Singh VP. In: Singh RK, Singh US, Khush GS, editors. Aromatic rices. New Delhi: Oxford and India Book House; 2000. p. 135–53.
Singh VK, Singh A, Singh SP, Ellur RK, Choudhary V, Sarkel S, Singh D, Gopala SK, Nagarajan M, Vinod KK, Singh UD, Rathore R, Prashanthi SK, Agrawal PK, Bhatt JC, Mohapatra T, Prabhu KV, Singh AK. Incorporation of blast resistance into “PRR78”, an elite Basmati rice restorer line through marker assisted backcross breeding. Field Crop Res. 2012a;128:8–16.
Singh A, Singh VK, Singh SP, Pandian RT, Ellur RK, Singh D, Bhowmick PK, Gopala Krishnan S, Nagarajan M, Vinod KK, Singh UD. Molecular breeding for the development of multiple disease resistance in Basmati rice. AoB Plants. 2012b;1:2012.
Singh VK, Singh A, Singh SP, Ellur RK, Singh D, Gopala Krishnan S, Bhowmick PK, Nagarajan M, Vinod KK, Singh UD, Mohapatra T, Prabhu KV, Singh AK. Marker-assisted simultaneous but stepwise backcross breeding for pyramiding blast resistance genes Pi2 and Pi54 into an elite Basmati rice restorer line PRR78. Plant Breed. 2013;132(5):486–95.
Su J, Wang W, Han J, Chen S, Wang C, Zeng L, Feng A, Yang J, Zhou B, Zhu X. Functional divergence of duplicated genes results in a novel blast resistance gene Pi50 at the Pi2/9 locus. Theor Appl Genet. 2015;128:2213–25.
Takagi H, Uemura A, Yaegashi H, Tamiru M, Abe A, Mitsuoka C, Utsushi H, Natsume S, Kanzaki H, Matsumura H, Saitoh H, Yoshida K, Cano LM, Kamoun S, Terauchi R. MutMap-Gap: whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii. New Phytol. 2013;200:276–83.
Takahashi A, Hayashi N, Miyao A, Hirochika H. Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging. BMC Plant Biol. 2010;10:175.
Talbot NJ. On the trail of a cereal killer: exploring the biology of Magnaporthe grisea. Annu Rev Microbiol. 2003;57:177–202.
Tanksley SD. Molecular markers in plant breeding. Plant Mol Biol Rep. 1983;1:1–3.
Tanweer F, Rafii M, Sijam K, Rahim H, Ahmed F, Latif M. Current advance methods for the identification of blast resistance genes in rice. C R Biol. 2015;338:321–34.
Telebanco-Yanoria MJ, Koide Y, Fukuta Y, Imbe T, Tsunematsu H, Kato H, Ebron LA, Nguyet TMN, Kobayashi N. A set of near-isogenic lines of Indica-type rice variety CO39 as differential varieties for blast resistance. Mol Breed. 2011;27:357–73.
Tsunematsu H, Yanoria MJT, Ebron LA, Hayashi N, Ando I, Kato H, Imbe T, Khush GS. Development of monogenic lines of rice for blast resistance. Breed Sci. 2000;50:229–34.
Wang Z, Yano M, Yamanouchi U, Iwamoto M, Monna L, Hayasaka H, Katayose Y, Sasaki T. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J. 1999;19:55–64.
Wang B, Ebbole DJ, Wang Z. The arms race between Magnaporthe oryzae and rice: diversity and interaction of Avr and R genes. J Integr Agric. 2017;16(12):2746–60.
Wilson RA, Talbot NJ. Under pressure: investigating the biology of plant infection by Magnaporthe oryzae. Nat Rev Microbiol. 2009;7(3):185–95.
Wu J, Kou Y, Bao J, Li Y, Tang M, Zhu X, Ponaya A, Xiao G, Li J, Li C, Song MY, Cumagun CJR, Deng Q, Lu G, Jeon JS, Naqvi NI, Zhou B. Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9 that triggers Pi9-mediated blast resistance in rice. New Phytol. 2015;206:1463–75.
Xu X, Hayashi N, Wang C, Fukuoka S, Kawasaki S, Takatsuji H, Jiang C. Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein. Mol Breed. 2014;34:691–700.
Yoshida K, Saitoh H, Fujisawa S, Kanzaki H, Matsumura H, Yoshida K, Tosa Y, Chuma I, Takano Y, Win J, Kamoun S, Terauchi R. Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. Plant Cell. 2009;21:1573–91.
Yuan B, Zhai C, Wang WJ, Zeng XS, Xu XK, Hu HQ, Lin F, Wang L, Pan QH. The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes. Theor Appl Genet. 2011;122:1017–28.
Zhai C, Lin F, Dong ZQ, He XY, Yuan B, Zeng XS, Wang L, Pan QH. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication. New Phytol. 2011;189:321–34.
Zhai C, Zhang Y, Yao N, Lin F, Liu Z, Dong Z, Wang L, Pan Q. Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance. PLoS One. 2014;9:e98067.
Zhang S, Wang L, Wu W, He L, Yang X, Pan Q. Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib. Sci Rep. 2015;5:11642.
Zheng Y, Zheng W, Lin F, Zhang Y, Yi Y, Wang B, Lu G, Wang Z, Wu W. AVR1-CO39 is a predominant locus governing the broad avirulence of Magnaporthe oryzae 2539 on cultivated rice (Oryza sativa L.). Mol Plant-Microbe Interact. 2011;24:13–7.
Zhou B, Qu SH, Liu GF, Dolan M, Sakai H, Lu GD, Bellizzi M, Wang GL. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant-Microbe Interact. 2006;19:1216–28.
Zhu X, Chen S, Yang J, Zhou S, Zeng L, Han J, Su J, Wang L, Pan Q. The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family. Theor Appl Genet. 2012;124:1295–304.
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Khanna, A. et al. (2021). Utilizing Host-Plant Resistance to Circumvent Blast Disease in Rice. In: Nayaka, S.C., Hosahatti, R., Prakash, G., Satyavathi, C.T., Sharma, R. (eds) Blast Disease of Cereal Crops. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-60585-8_2
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