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Single-Domain Antibodies

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Greg Hussack

    1. Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Kevin A. Henry

    1. Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2446)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction to Single-Domain Antibodies

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications

      • Fangling Ji, Jun Ren, Cécile Vincke, Lingyun Jia, Serge Muyldermans
      Pages 3-17

    3. Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development

      • Samata S. Pandey, Marina Kovaleva, Caroline J. Barelle, Obinna C. Ubah
      Pages 19-33

  3. Isolation of Single-Domain Antibodies

    1. Front Matter

      Pages 35-35
      Download chapter PDF

    2. Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders

      • Frédéric Trempe, Martin A. Rossotti, Tahir Maqbool, C. Roger MacKenzie, Mehdi Arbabi-Ghahroudi
      Pages 37-70

    3. Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders

      • Jahaziel Gasperin-Bulbarela, Olivia Cabanillas-Bernal, Salvador Dueñas, Alexei F. Licea-Navarro
      Pages 71-93

    4. Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets

      • Kaitlyn Bacon, Stefano Menegatti, Balaji M. Rao
      Pages 95-119

    5. A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications

      • Dubravka Drabek, Rick Janssens, Rien van Haperen, Frank Grosveld
      Pages 121-141

  4. Expression of Single-Domain Antibodies

    1. Front Matter

      Pages 143-143
      Download chapter PDF

    2. Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase

      • Ario de Marco
      Pages 145-157

    3. Small-Scale Secretory VHH Expression in Saccharomyces cerevisiae

      • Michiel M. Harmsen, Marga van Hagen-van Setten, Peter T. J. Willemsen
      Pages 159-179

    4. Production of Single-Domain Antibodies in Pichia pastoris

      • Yusei Matsuzaki, Kaho Kajiwara, Wataru Aoki, Mitsuyoshi Ueda
      Pages 181-203

    5. Production of Designer VHH-Based Antibodies in Plants

      • Henri De Greve
      Pages 205-230

  5. Single-Domain Antibody Engineering and Characterization

    1. Front Matter

      Pages 231-231
      Download chapter PDF

    2. Assessing the Aggregation Propensity of Single-Domain Antibodies upon Heat-Denaturation Employing the ΔTm Shift

      • Patrick Kunz
      Pages 233-244

    3. Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing

      • Michael J. Lowden, Henk van Faassen, Shalini Raphael, Shannon Ryan, Greg Hussack, Kevin A. Henry
      Pages 245-268

    4. Engineering pH-Sensitive Single-Domain Antibodies

      • Tosha M. Laughlin, James R. Horn
      Pages 269-298

    5. Humanization of Camelid Single-Domain Antibodies

      • Traian Sulea
      Pages 299-312

    6. Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues

      • Paul J. Wichgers Schreur, Sandra van de Water, Jeroen Kortekaas
      Pages 313-326
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Keywords

About this book

This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.

Editors and Affiliations

  • Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Canada

    Greg Hussack, Kevin A. Henry

Bibliographic Information

  • Book Title: Single-Domain Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Greg Hussack, Kevin A. Henry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2075-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2074-8Published: 15 February 2022

  • Softcover ISBN: 978-1-0716-2077-9Published: 15 February 2022

  • eBook ISBN: 978-1-0716-2075-5Published: 14 February 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 612

  • Number of Illustrations: 11 b/w illustrations, 84 illustrations in colour

  • Topics: Biological Techniques

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