Abstract
Multimerization of single-domain antibodies (sdAbs) is instrumental for construction of antibody molecules with high avidity, extended in vivo half-life, and tailor-made biological activity. Two-component superglues, based on bacterium-derived peptides (Tags) and small protein domains (Catchers) that form isopeptide bonds when in close proximity, enable the creation of multimers by simply mixing of the individual components. Here, we provide detailed methods for the construction of sdAbs and scaffolds bearing genetically fused superglue components and their assembly into multimeric complexes.
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Acknowledgments
We thank Dr. Michiel Harmsen for his careful reading of the protocol.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Wichgers Schreur, P.J., van de Water, S., Kortekaas, J. (2022). Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues. In: Hussack, G., Henry, K.A. (eds) Single-Domain Antibodies. Methods in Molecular Biology, vol 2446. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2075-5_15
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DOI: https://doi.org/10.1007/978-1-0716-2075-5_15
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Publisher Name: Humana, New York, NY
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