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Triticale Isolated Microspore Culture for Doubled Haploid Production

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2287))

Abstract

Here, we describe a method of triticale isolated microspore culture for production of doubled haploid plants via androgenesis. We use this method routinely because it is highly efficient and works well on different triticale genotypes. To force microspores into becoming embryogenic, we apply a 21-day cold pretreatment. The shock of cold facilitates redirecting microspores from their predestined pollen developmental program into the androgenesis pathway. Ovaries are included in our culture methods to help with embryogenesis, and the histone deacytelase inhibitor Trichostatin A (TSA) is added to further improve androgenesis and increase our ability to recover green doubled haploid plants.

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Acknowledgments

We thank Katherine Anderson-Bain and Jamieson Peacock for proofreading and Fengying Jiang for technical assistance.

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Authors and Affiliations

  1. Agriculture and Agri-food Canada, Lethbridge, AB, Canada

    Priti Maheshwari & John D. Laurie

Authors
  1. Priti Maheshwari
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  2. John D. Laurie
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Corresponding author

Correspondence to John D. Laurie .

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Editors and Affiliations

  1. CPI, COMAV - Univ Politecnica Valencia, Valencia, Valencia, Spain

    Jose M. Segui-Simarro

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Maheshwari, P., Laurie, J.D. (2021). Triticale Isolated Microspore Culture for Doubled Haploid Production. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2287. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1315-3_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1315-3_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1314-6

  • Online ISBN: 978-1-0716-1315-3

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