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Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

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Abstract

Isolated microspore culture (IMC) has been used to develop doubled haploid lines and to generate homozygous lines in a single generation for varietal development. Phytosulfokine has been previously used in promoting cell growth and embryo development in various systems. In this study, phytosulfokine alpha (PSK-α) supplemented IMC induction medium was evaluated in triticale and wheat cultivars, and the production of embryo-like structures (ELS), green and albino plants were recorded. In addition, the contribution of ovary co-culture was also evaluated in IMC on a NPB99 + 10F induction medium. Over a range of concentrations up to 10−7M, PSK-α yielded more ELS and green plants in wheat and triticale cultivars at the highest dose, when compared to the control. It also minimized albinism in wheat cultivars, but not in triticale. Interestingly, 10−7M PSK-α also supported the formation of a large number of embryos and a few green plants in the absence of nursing ovaries.

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Abbreviations

PSK-α:

Phytosulfokine alpha

DH:

Doubled haploid

ELS:

Embryo like structure

IMC:

Isolated microspore culture

SSD:

Single seed descent

PCD:

Programmed cell death

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB, T1J 4B1, Canada

    Muhammad Asif, François Eudes, Harpinder Randhawa & Eric Amundsen

  2. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Muhammad Asif & Dean Spaner

Authors
  1. Muhammad Asif
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  2. François Eudes
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  3. Harpinder Randhawa
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  4. Eric Amundsen
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  5. Dean Spaner
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Correspondence to François Eudes.

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Asif, M., Eudes, F., Randhawa, H. et al. Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat. Plant Cell Tiss Organ Cult 116, 125–130 (2014). https://doi.org/10.1007/s11240-013-0379-y

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  • DOI: https://doi.org/10.1007/s11240-013-0379-y

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