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Metabolic Engineering of Isoprenoid Biosynthesis

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Natural Products

Abstract

Isoprenoids constitute one of the largest families of natural compounds. They play essential functions in plant growth and development and furnish compounds of high interest for humans. Here, we present the current knowledge on isoprenoid metabolism before describing the strategies that have been used for isoprenoid metabolic engineering. We discuss the advantages and drawbacks of using microorganisms and plants as cell platform for the production of isoprenoids of interest.

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Abbreviations

ADS:

Amorpha 4,11-diene synthase

CRY2:

Cryptochrome 2

DET1:

De-etiolated 1

DMAPP:

Dimethylallyl diphosphate

DXP:

1-deoxy-d-xylulose 5-phosphate

DXR:

1-deoxy-d-xylulose 5-phosphate

FPP:

Farnesyl diphosphate

FPS:

Farnesyl diphosphate synthase

GGPP:

Geranyl geranyl diphosphate

GGPS:

Geranyl geranyl diphosphate synthase

GPP:

Geranyl diphosphate

GPS:

Geranyl diphosphate synthase

HMG:

3-hydroxyl-3-methylglutaryl

HMGR:

3-hydroxyl-3-methylglutaryl reductase

IDS:

Isoprenyl diphosphate synthase

IPP:

Isopentenyl diphosphate

IS:

Isoprene synthase

MEP:

Methyl-d-erythritol 4-phosphate

MGT:

Multigene transfer

MVA:

Acetate/mevalonate

NES1:

Nerolidol synthase

NPP:

Neryl diphosphate

PSY:

Phytoene synthase

PTS:

Prenyltransferase

SqS:

Squalene synthase

TPS:

Terpene synthase

VOCS:

Volatile organic compounds.

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Acknowledgments

We wish to thank Dr Sophie Colombié for critical reading of this manuscript. Dr Anne Pribat was in receipt of a grant from the French National Research Agency (ANR) in the frame of the “Polyterp” project.

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Authors and Affiliations

  1. Université de Bordeaux 1, UMR 1332 de Biologie du Fruit et Pathologie, 71 Avenue E Bouleaux, BP 81, Villenave d’Ornon, France

    Anne Pribat, Lisa Boureau, Linda S. Bert & Dr. Philippe Gallusci

  2. Université de Bordeaux 1, INRA, UMR 1332 de Biologie du Fruit et Pathologie, 71 Avenue E Bouleaux, BP 81, Villenave d’Ornon, France

    Anne Mortain-Bertrand, Dominique Rolin & Emeline Teyssier

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  1. Anne Pribat
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  2. Lisa Boureau
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  3. Anne Mortain-Bertrand
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  4. Linda S. Bert
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  6. Emeline Teyssier
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  7. Dr. Philippe Gallusci
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Correspondence to Philippe Gallusci .

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  1. Botany Department, M.L. Sukhadia University, Durga Nursery Road, Udaipur, 313001, Rajasthan, India

    Kishan Gopal Ramawat

  2. Institute of Vine and Wine Sciences, Biological-Active Plant Substances Study, University of Bordeaux, 210 Chemin de Leysotte CS 50008, Villenave d'Ornon, 33882, France

    Jean-Michel Mérillon

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Pribat, A. et al. (2013). Metabolic Engineering of Isoprenoid Biosynthesis. In: Ramawat, K., Mérillon, JM. (eds) Natural Products. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_126

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