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Cytogenetic Analyses of Arabidopsis

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Arabidopsis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 323))

Abstract

For a long time, Arabidopsis thaliana was not a favorite of cytogeneticists because of its small chromosomes. Nevertheless, most cytogenetic approaches have become applicable to this model plant during the past decade. Recently, the very small genome, the low content, and strong clustering of repetitive sequences even turned out to be advantageous for the first establishment of chromosome painting in a euploid plant. Chromosome painting together with the high-resolution fluorescence in situ hybridization (FISH) mapping on Arabidopsis pachytene chromosomes has opened new perspectives for plant cytogenetics.

In this chapter, we describe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of Arabidopsis chromosomes, and comparative chromosome painting in other Brassicaceae species.

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Author information

Authors and Affiliations

  1. Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, UK

    Martin Lysak

  2. Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany

    Paul Fransz & Ingo Schubert

Authors
  1. Martin Lysak
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  2. Paul Fransz
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  3. Ingo Schubert
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Editor information

Editors and Affiliations

  1. Dept. of Biotechnology, Instituto Nacional de Investigacion y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain

    Julio Salinas PhD

  2. Dept. de Genetica Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus de Cantoblanco, Madrid, Spain

    Jose J. Sanchez-Serrano PhD

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© 2006 Humana Press Inc.

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Lysak, M., Fransz, P., Schubert, I. (2006). Cytogenetic Analyses of Arabidopsis . In: Salinas, J., Sanchez-Serrano, J.J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 323. Humana Press. https://doi.org/10.1385/1-59745-003-0:173

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  • DOI: https://doi.org/10.1385/1-59745-003-0:173

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-395-4

  • Online ISBN: 978-1-59745-003-4

  • eBook Packages: Springer Protocols

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