Summary
Arabidopsis thaliana is a small flowering plant of the mustard family. It has a four to five week generation time, can be self- or cross-pollinated and bears as many as 104 seeds per plant. Many visible and biochemical mutations exist and have been mapped by recombination to one of the five chromosomes that comprise the haploid karyotype. With the experiments reported here we demonstrate that Arabidopsis has an extraordinarily small haploid genome size (approximately 7×107 nucleotide pairs) and a low level of cytosine methylation for an angiosperm. In addition, it appears to have little repetitive DNA in its nuclear DNA, in contrast to other higher plants.
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References
Aerts M, Jacobs M, Hernalsteens J-P, van Montagu M, Schell J (1979) Induction and in vitro culture of Arabidopsis thaliana crown gall tumours. Plant Sci Lett 17:43–50
Bedbrook JR, Kolodner R (1979) The structure of chloroplast DNA. Annu Rev Plant Physiol 30:593–620
Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Proc R Soc Lond B 274:227–274
Bolivar F (1978) Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–136
Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. In: Grossman EL, Moldave K (eds) Methods in enzymology, vol 29E. Academic Press, New York, pp 363–406
Cairns J (1963) The chromosome of E. coli. Cold Spring Harbor Symp Quant Biol 28:43–46
Crain WR, Eden FC, Pearson WR, Davidson EH, Britten RJ (1976) Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma (Berl) 56:309–326
Diala E, Plant M, Coalson D, Hoffman R (1981) DNA methylation in normal and SV40-transformed human fibroblasts. Biochem Biophys Res Commun 102:1379–1384
Diala E, Hoffman R (1982) Hypomethylation of HeLa cell DNA and the absence of 5-methylcytosine in SV40 and adenovirus (type 2) DNA: Analysis by HPLC. Biochem Biophys Res Commun 107:19–26
Felsenfeld G (1971) Analysis of temperature-dependent absorption spectra of nucleic acids. In: Cantoni GL, Davies DR (eds) Procedures in nucleic acid research, vol 2. Harper and Row Publishers, New York, pp 233–244
Flavell R (1980) The molecular characterization and organization of plant chromosomal DNA sequences. Annu Rev Plant Physiol 31:569–596
Gruenbaum Y, Navah-Many T, Cedar H, Razin A (1981) Sequence specificity of methylation in higher plant DNA. Nature 292:860–862
Kolodner R, Tewari KK (1975) The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta 402:372–390
Koorneef M, van Eden J, Hanhart CJ, Stam P, Braaksma FJ, Feenstra WJ (1983) Linkage map of Arabidopsis thaliana. J Hered 74:265–272
Kung S-D (1977) Expression of chloroplast genomes in higher plants. Annu Rev Plant Physiol 28:401–437
Laird CD (1971) Chromatid structure: Relationship between DNA content and nucleotide sequence diversity. Chromosoma (Berl) 32:378–406
Laird CD, McCarthy BJ (1969) Molecular characterization of the Drosophila genome. Genetics 63:865–882
Lauer GD, Roberts TM, Klotz LC (1977) Determination of the nuclear DNA content of Saccharomyces cerevisiae and implications for the organization of DNA in yeast chromosomes. J Mol Biol 114:507–526
Lebacq P, Vedel F (1981) Sall restriction enzyme analysis of chloroplast and mitochondrial DNAs in the genus Brassica. Plant Sci Lett 23:1–9
Link G, Chambers SE, Thompson JA, Falk H (1981) Size and physical organization of chloroplast DNA from mustard (Sinapis alba L.) Mol Gen Genet 181:454–457
Manning JE, Schmid CW, Davidson N (1975) Interspersion of repetitive and norepetitive DNA sequences in the Drosophila melanogaster genome. Cell 4:141–155
Pearson WR, Davidson EH, Britten RJ (1977) A program for least squares analysis of reassociation and hybridization data. Nucl Acids Res 4:1727–1737
Rédei GP (1970) Arabidopsis thaliana (L.) Heynh: A review of the genetics and biology. Biblio Genetica 20:1–127
Rédei GP (1973) Extra chromosomal mutability determined by a nuclear gene locus in Arabidopsis. Mutat Res 18:149–162
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Schachat FH, Hogness DS (1973) Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 38:371–381
Shapiro HS (1976) Distribution of purines and pyrimidines in deoxyribonucleic acids. CRC Handbook Biochem Molec Biol Nucl Acids 2:241–281
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sulston JE, Brenner S (1974) The DNA of Caenorhabditis elegans. Genetics 77:95–104
Thomas AJ, Sherrat HSA (1956) The isolation of nucleic acid fractions from plant leaves and their purine and pyrimidine composition. Biochem J 62:1–4
Urieli-Shoval S, Gruenbaum Y, Sedat J, Razin A (1982) The absence of detectable methylated bases in Drosophila melanogaster DNA. FEBS Lett 146:148–152
Vanyushin BF, Belozerskii AN (1959) Nucleotide composition of the desoxyribonucleotides of higher plants. Dokl Akad Nauk USSR 129:944–946
Vedel F, Mathieu C (1982) Isolation of purified mitochondrial DNA from Brassicae. Anal Biochem 127:1–8
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Communicated by R.B. Goldberg
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Leutwiler, L.S., Hough-Evans, B.R. & Meyerowitz, E.M. The DNA of Arabidopsis thaliana . Molec. Gen. Genet. 194, 15–23 (1984). https://doi.org/10.1007/BF00383491
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DOI: https://doi.org/10.1007/BF00383491