Abstract
An atlas of meiosis inArabidopsis thaliana, encompassing all stages from preleptotene to telophase II and early microspore formation, is presented in detail for the first time. The photomicrographs and descriptions are based on staining with the DNA fluorochrome 4′,6-diamidino-2-phenylindole (DAPI) combined with a spreading procedure, or haematoxylin-iron alum (HIA) staining. Despite previous reservations about the practicality of cytogenetic meiotic analysis inArabidopsis due to its small genome size, good-quality and clearly analysable preparations of all meiotic stages were obtained. This atlas of normal, wild-type meiosis is considered an essential prerequisite to informed analyses of meiotic mutants. Furthermore, extended prophase I chromosomes, particularly at the pachytene stage, offer considerable potential for producing a detailed cytogenetic map (karyotype) ofArabidopsis chromosomes with the additional prospect of high-resolution physical mapping based on fluorescencein situ hybridization (FISH) of defined DNA probes to these extended chromosomes.
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References
Albini SM (1994) A karyotype of theArabidopsis thaliana genome derived from synaptonemal complex analysis at prophase I of meiosis.Plant J 5: 665–672.
Albini SM, Jones GH, Parker, JS (1992) Synaptonemal complex spreading: an ultrastructural approach to chromosome analysis inArabidopsis thaliana.AFRC/PMB Arabidopsis Newsletter8: 5.
Ambros P, Schweizer D (1976) The Giemsa C-banded karyotype ofArabidopsis thaliana (L.) HEYN.Arabidopsis Information Service 13: 167–171.
Darlington CD (1932)Recent Advances in Cytology. London: Churchill.
De Jong H, Oud JL (1979) Location and behaviour of constitutive heterochromatin during meiotic prophase inBeta vulgaris L.Genetica 51: 125–133.
Dennis E, Caboche M, Flavell RB et al. (1994) The Multinational CoordinatedArabidopsis thaliana Genome Research Project. Progress Report Year 4. Arlington, VA: National Science Foundation.
Flavell RB, Dean C, Moore G (1993) Exploring plant genomes, small and large. In: Heslop-Harrison JS, Flavell RB eds.The Chromosome. Oxford: Bios Scientific Publishers, pp 249–264.
Hauge B, Hanley S, Cartinhour et al. (1993) An integrated genetic/RFLP map of theArabidopsis thaliana genome.Plant J 3: 745–754.
Henderson SA, Lu BC (1968). The use of haematoxylin for squash preparations of chromosomes.Stain Technology 43: 233–236.
John B (1990)Meiosis. Cambridge: Cambridge University Press.
Jones GH, Heslop-Harrison JS (1996) Classical and molecular cytogenetics ofArbidopsis thaliana. In: Mulligan B, ed. Arabidopsis:A Practical Approach. Oxford: Oxford University Press.
Klasterska I (1976) A new look on the role of diffuse stage in cytological problems of plant and animal meiosis.Hereditas 82: 193–204.
Klasterska I, Ramel C (1980) Meiosis in PMCs ofArabidopsis thaliana. ArabidopsisInformation Service 17: 1–10.
Lima de Faria A (1956) The role of the kinetochore in chromosome organization.Hereditas 42: 85–160.
Maluszynska J, Heslop-Harrison JS (1991) Localization of tandemly repeated DNA sequences inArabidopsis thaliana.Plant J 1: 159–166.
Meyerowitz E (1987)Arabidopsis thaliana.Annu Rev Genet 21: 93–111.
Ramulu KS, Sybenga J (1979) Comparison of fast neutrons and X-rays in respect of genetic effects accompanying induced chromosome aberrations: induction and analysis of aberrations inArabidopsis thaliana.Arabidopsis Information Service 16: 27–34.
Schweizer D, Ambros P, Grundler P, Varga F (1987) Attempts to relate cytological and molecular chromosome data ofArabidopsis thaliana to its genetic linkage map.Arabidopsis Information Service 25: 27–34.
Schmidt R, West J, Love K et al. (1995) Physical map and organization ofArabidopsis thaliana chromosome 4.Science 270: 480–483.
Steinitz-Sears LM (1963) Chromosome studies inArabidopsis thaliana.Genetics 48: 483–490.
Steinitz-Sears LM (1968) Notes on a cytological technique forArabidopsis. In:Effects of Radiation on Meiotic Systems. Vienna: International Atomic Agency, pp. 157–158.
Steinitz-Sears LM, Lee-Chen S (1970). Cytogenetic studies inArabidopsis thaliana.Can J Genet Cytol 12: 217–223.
Sharp LW (1921)An Introduction to Cytology. New York: McGraw-Hill.
Vieira MLC, Briarty LG, Mulligan BJ (1990) A method for analysis of meiosis in anthers ofArabidopsis thaliana.Ann Bot 66: 717–719.
Vizir IY, Korol AB (1990) Sex difference in recombination frequency inArabidopsis.Heredity 65: 379–383.
Zhong X-B, de Jong H, Zabel P (1996) Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes, suitable for fluorescencein situ hybridization (FISH).Chrom Res 4: 24–28.
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accepted for publication by J. S. (Pat) Heslop-Harrison
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Ross, K.J., Fransz, P. & Jones, G.H. A light microscopic atlas of meiosis inArabidopsis thaliana . Chromosome Res 4, 507–516 (1996). https://doi.org/10.1007/BF02261778
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DOI: https://doi.org/10.1007/BF02261778