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Biosensor for bisphenol A leaching from baby bottles using a glassy carbon electrode modified with DNA and single walled carbon nanotubes

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Abstract

We have developed a biosensor for highly sensitive and selective determination of the endocrinic disruptor bisphenol A (BPA). It is based on glassy carbon electrode modified with calf thymus DNA and a composited prepared from single walled carbon nanotubes (SWNT) and Nafion. The interaction between BPA and DNA was studied by voltammetry. The binding constant was determined to be 3.55 × 103 M−1, and the binding site has a length of 4.3 base pairs. These electrochemical studies provide further information for a better understanding of the toxicity and carcinogenicity of BPA. Under optimal conditions, the biosensor displays a linear electrochemical response to BPA in the 10 nM to 20 μM concentration range, with a detection limit as low as 5.0 nM (at an S/N of 3). The method was successfully applied to the quantification of BPA in leachates from plastic baby bottles. Recoveries range from 94.0 % to 106.0 % which underpins the excellent performance of this SWNT-based DNA sensor.

A biosensor based on DNA and single walled carbon nanotubes modified glassy carbon electrode displays a linear electrochemical response to bisphenol A in the 10 nM to 20 μM concentration range, with a detection limit as low as 5.0 nM (at an S/N of 3).

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Acknowledgments

The authors gratefully acknowledge financial support from the Key project of Shenzhen Polytechnic (No.2210 K3070014) and “Qianbaishi Candidate” fund for Higher Education of Guangdong Province.

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Authors and Affiliations

  1. School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, Guangdong, 518055, China

    Xiaohua Jiang, Wengjie Ding & Chonglin Luan

  2. Faculty of Materials Science and Chemical Engineering, The State Key Laboratroy Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, Zhejiang, 315211, China

    Qingqing Ma & Zhiyong Guo

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  1. Xiaohua Jiang
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  2. Wengjie Ding
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  3. Chonglin Luan
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  4. Qingqing Ma
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Correspondence to Xiaohua Jiang.

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Jiang, X., Ding, W., Luan, C. et al. Biosensor for bisphenol A leaching from baby bottles using a glassy carbon electrode modified with DNA and single walled carbon nanotubes. Microchim Acta 180, 1021–1028 (2013). https://doi.org/10.1007/s00604-013-1025-4

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  • DOI: https://doi.org/10.1007/s00604-013-1025-4

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