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Electrochemical aptasensor for the determination of bisphenol A in drinking water

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Abstract

We present an electrochemical aptasensor for rapid and ultrasensitive determination of the additive bisphenol A (BPA) and for screening drinking water for the presence of BPA. A specific aptamer against BPA and its complementary DNA probe were immobilized on the surface of a gold electrode via self-assembly and hybridization, respectively. The detection of BPA is mainly based on the competitive recognition of BPA by the immobilized aptamer on the surface of the electrode. The electrochemical aptasensor enables BPA to be detected in drinking water with a limit of detection as low as 0.284 pg mL−1 in less than 30 min. This extraordinary sensitivity makes the method a most powerful tool for on-site monitoring of water quality and food safety.

A novel electrochemical aptasensor was developed for rapid and ultrasensitive detection of bisphenol A (BPA) and screening of BPA in drinking water using the specific aptamer against BPA.

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Acknowledgments

This research was financially supported by the Huangshan Young Scholar Fund of Hefei University of Technology (407-037025), Science and Technology Research Project of General Administration of QSIQ of P. R. China (201210127), the 12th Five Years Key Programs (2012BAK17B10, SS2012AA101001), the foundation for the author of national excellent doctoral dissertation of China (No. 201187), National Science Foundation of China (No. 20906039), and the Fundamental Research Funds for the Central Universities (2012HGCX0003).

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Authors and Affiliations

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Feng Xue, Jingjing Wu, Huaqin Chu, Zhanlong Mei, Yongkang Ye, Jian Liu, Lei Zheng & Wei Chen

  2. Animal, Plant & Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, 200001, People’s Republic of China

    Feng Xue & Rui Zhang

  3. School of Food Science & Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Chifang Peng

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  1. Feng Xue
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  9. Lei Zheng
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Correspondence to Lei Zheng or Wei Chen.

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Feng Xue, Jingjing Wu and Huaqin Chu contributed equally to this work.

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Xue, F., Wu, J., Chu, H. et al. Electrochemical aptasensor for the determination of bisphenol A in drinking water. Microchim Acta 180, 109–115 (2013). https://doi.org/10.1007/s00604-012-0909-z

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