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The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO

  • Applied microbial and cell physiology
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Abstract

The effects of malachite green (MG) on the bacterial community in Antarctic soil were assessed. Culture-independent community analysis using 16S rRNA gene pyrosequencing showed that, in the presence of MG, the relative abundance of Pseudomonas dramatically increased from 2.2 % to 36.6 % (16.6-fold), and Pseudomonas became the predominant genus. The reduction in bacterial biodiversity was demonstrated by diversity indices and rarefaction curves. MG-degrading Pseudomonas sp. MGO was isolated from Antarctic soil. MG tolerance and decolorization activity were confirmed by growth, spectrophotometric, high-performance liquid chromatography, and thin-layer chromatography analyses in high MG concentrations. Our data showed that the decolorization process occurred via biodegradation, while biosorption also occurred after some time during the fed-batch decolorization process. Significant inductions in laccase, nicotinamide adenine dinucleotide–2,6 dichlorophenol indophenol reductase, and MG reductase activities suggested their involvement in the decolorization process. We also showed that the high tolerance of strain MGO to toxic MG might be mediated by upregulation of oxidative stress defense systems such as superoxide dismutase and protease. Collectively, these results demonstrated the response of the Antarctic soil bacterial community to MG and provided insight into the molecular mechanism of MG-tolerant Pseudomonas strains isolated from Antarctic soil.

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Acknowledgment

This work was supported by a grant (2012-0005277) from the MEST/NRF program. Dr. W. Park was supported by the LG Yonam Foundation, Seoul, South Korea.

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Authors and Affiliations

  1. Department of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-713, Republic of Korea

    Jaejoon Jung, Hyoju Seo & Woojun Park

  2. Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea

    Se Hee Lee & Che Ok Jeon

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  1. Jaejoon Jung
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  2. Hyoju Seo
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Correspondence to Woojun Park.

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Jung, J., Seo, H., Lee, S.H. et al. The effect of toxic malachite green on the bacterial community in Antarctic soil and the physiology of malachite green-degrading Pseudomonas sp. MGO. Appl Microbiol Biotechnol 97, 4511–4521 (2013). https://doi.org/10.1007/s00253-012-4669-9

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