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Decolorization of azo dyes by Shewanella oneidensis MR-1 in the presence of humic acids

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Abstract

The effects of humic acid (HA) on azo dye decolorization by Shewanella oneidensis MR-1 were studied. It was found that HA species isolated from different sources could all accelerate the decolorization of Acid Red 27 (AR27). Anoxic and anaerobic conditions were required for the enhancement of azo dye decolorization by HA. In the presence of 50 mg DOC L−1 Aldrich HA, 15–29% increases in decolorization efficiencies of azo dyes with different structures were achieved in 11 h. The enhancing effects increased with the increase of HA concentrations ranging from 25 to 150 mg DOC L−1, and the decolorization rates were directly proportional to the HA concentrations when they were below 100 mg DOC L−1. Lactate and formate were good electron donors for AR27 decolorization in the presence of HA. Both nitrate (0.1–3.0 mM) and nitrite (0.3–1.2 mM) inhibited AR27 decolorization in the presence of HA, and negligible decolorization was observed before their removal. Soluble FeCl3 could accelerate the decolorization process in the presence of HA, whereas insoluble hematite could not. These findings may affect the understanding of bioremediation of azo dye-polluted environments and help improve the treatment of azo dye wastewaters.

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Acknowledgements

The work was financially supported by the National Natural Science Foundation of China (No. 51008044 and 50978040), Fundamental Research Funds for the Central Universities, China Postdoctoral Science Foundation (20100471435 and 200902538), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0814), and National Key Scientific and Technology Project for Water Pollution Treatment of China (2008ZX07208-004-2).

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  1. Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), State Key Laboratory of Fine Chemicals, School of Environmental Science and Technology, School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, China

    Guangfei Liu, Jiti Zhou, Jing Wang, Xiujuan Wang, Ruofei Jin & Hong Lv

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  1. Guangfei Liu
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  2. Jiti Zhou
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  3. Jing Wang
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Correspondence to Jing Wang.

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Liu, G., Zhou, J., Wang, J. et al. Decolorization of azo dyes by Shewanella oneidensis MR-1 in the presence of humic acids. Appl Microbiol Biotechnol 91, 417–424 (2011). https://doi.org/10.1007/s00253-011-3273-8

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