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Hydra: Research Methods

  • Book
  • © 1983

Overview

Editors:
  1. Howard M. Lenhoff

    1. Department of Developmental and Cell Biology, University of California, Irvine, USA

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Table of contents (62 chapters)

  1. Manipulating Tissue Organization

    1. Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells

      • Kristine M. Flick, Hans R. Bode
      Pages 251-259

    2. Culturing Interstitial Stem Cells in Hydra Aggregates

      • Charles N. David
      Pages 261-266

    3. Separating Viable Tissue Layers

      • Georgia E. Lesh-Laurie
      Pages 267-271

    4. Preparing Ectoderm/Endoderm Chimeras

      • Nancy Wanek
      Pages 273-277

  2. Manipulating Cellular Composition in Vivo

    1. Front Matter

      Pages 279-279
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    2. Eliminating All Nonepithelial Cells Using Colchicine

      • Beverly A. Marcum, Richard D. Campbell
      Pages 281-286

    3. Culturing Epithelial Hydra

      • Beverly A. Marcum
      Pages 287-290

    4. Reducing Populations of Interstitial Cells and Nematoblasts with Hydroxyurea

      • Hans R. Bode
      Pages 291-294

    5. Preparing Hydra viridis with Nerve Cells and No Interstitial Cells, or with Neither of These Cell Types

      • Patricia Novak
      Pages 295-297

    6. Eliminating Interstitial Cells with Nitrogen Mustard

      • Charles N. David
      Pages 299-302

    7. Altering Cell Population Levels by Gamma Irradiation

      • Cheng-Mei Fradkin
      Pages 303-304

    8. Reducing Number of Nematocytes in the Tentacles

      • G. Scott Smith, Hans R. Bode
      Pages 305-307

  3. Assay and Isolation of Substances Controlling Morphogenesis in Hydra

    1. Front Matter

      Pages 309-309
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    2. Assay and Isolation of Substances Controlling Morphogenesis in Hydra

      • H. Chica Schaller, Cornelis J. P. Grimmelikhuijzen, Tobias Schmidt
      Pages 311-324

  4. Isolation and/or Properties of Acellular Mesoglea and Nematocysts

    1. Front Matter

      Pages 325-325
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    2. Isolating Mesolamellae

      • Robert M. Day, Howard M. Lenhoff
      Pages 327-329

    3. Isolating Undischarged and Discharged Nematocysts from Acontiate Sea Anemones

      • Richard S. Blanquet
      Pages 331-333

    4. Dissolving the Nematocyst Capsule Wall and Identifying its Protein Component(s)

      • Richard S. Blanquet
      Pages 335-339

    5. Purifying an Inhibitor of Succinoxidase Activity from Hydra littoralis

      • Edward S. Kline, Vaman S. Waravdekar
      Pages 341-346

    6. Assays for Activities of Nematocyst Venoms and Their Components

      • David A. Hessinger
      Pages 347-358
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Editors and Affiliations

  • Department of Developmental and Cell Biology, University of California, Irvine, USA

    Howard M. Lenhoff

Bibliographic Information

  • Book Title: Hydra: Research Methods

  • Editors: Howard M. Lenhoff

  • DOI: https://doi.org/10.1007/978-1-4757-0596-6

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1983

  • Softcover ISBN: 978-1-4757-0598-0Published: 23 May 2013

  • eBook ISBN: 978-1-4757-0596-6Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XXXI, 463

  • Number of Illustrations: 134 b/w illustrations

  • Topics: Medical Microbiology

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