Skip to main content

Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells

  • Chapter
Hydra: Research Methods

Abstract

To dissociate hydra tissue into a suspension of viable cells, and to form from those cells aggregates of tissue that will develop into viable whole hydra.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Campbell, R. D. 1976. Elimination of Hydra interstitial and nerve cells by means of colchicine. J. Cell Sci. 21: 1–13.

    PubMed  CAS  Google Scholar 

  • David, C. D., and Murphy, S. 1977. Characterization of interstitial stem cells in Hydra by cloning. Del,. Biol. 58: 372–383.

    CAS  Google Scholar 

  • Diehl, F. A., and Burnett, A. L. 1964. The role of interstitial cells in the maintenance of Hydra. I. Specific destruction of interstitial cells to normal, asexual, and non-budding animals. J. Exp. Zool. 155: 253–259.

    Article  PubMed  CAS  Google Scholar 

  • Gierer, A., Berking, S., Bode, H., David, C. N., Flick, K., Hansmann, G., Schaller, H., and Trenkner, E. 1972. Regeneration of Hydra from reaggregated cells. Nature (London) New Biol. 239: 98–101.

    Article  CAS  Google Scholar 

  • Trenkner, E., Flick, K., Hansmann, G., Bode, P., and Bode, H. 1973. Studies on Hydra cells in vitro. J. Exp. Zool. 185: 317–326.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer Science+Business Media New York

About this chapter

Cite this chapter

Flick, K.M., Bode, H.R. (1983). Dissociating Tissues into Cells and the Development of Hydra from Aggregated Cells. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_33

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics