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Co-localization of corticotropin-releasing factor and vasopressin in median eminence neurosecretory vesicles

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Abstract

Vasopressin (VP) potentiates the effect of corticotropin-releasing factor (CRF) on the secretion of adrenocorticotropic hormone (ACTH) from anterior pituitary cells in vitro1–3, and both CRF4–7 and VP6,8 have been found in portal blood. These data support the hypothesis that VP acts synergistically with CRF to cause the secretion of ACTH in vivo1–4,8,9 but the origin of the CRF and VP, and the physiology of their release, have not been precisely defined. Parvocellular cell bodies in the paraventricular nucleus (PVN) which project to the external zone of the median eminence can be stained for both CRF and VP after adrenalectomy10–12, and there is light microscopic imunocytochemical evidence that neurophysin (NP) may be located within some of the CRF-containing axons13. Electron microscopic immunocytochemical studies have demonstrated the presence of CRF14–16, VP17,18 and its ‘carrier’ protein, VP-associated neurophysin (NP-VP)17,18 in 100-nm neurosecretory vesicles (NSVs) in axons terminating near the portal capillary plexus in the external zone of the median eminence. If these peptides are extensively co-localized in the same NSVs in the median eminence, then coordinate secretion of CRF and VP in vivo is obligatory, at least in some physiological circumstances. We demonstrate in this report, using post-embedding electron microscopic immunocytochemistry on serial ultrathin sections, that CRF, VP and NP-VP are contained not only in the same axons and terminals, but in the same 100-nm NSVs in the median eminence of both normal and adrenalectomized rats. In addition, in the normal rat median eminence 44% of the CRF-positive axons and terminals stained strongly for VP and NP-VP, whereas in the adrenalectomized rat virtually all the CRF-positive structures in the median eminence showed strong staining for VP and NP-VP, indicating a transformation of one subpopulation of CRF-positive axons and terminals by adrenalectomy.

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References

  1. Gillies, G. E., Linton, E. A. & Lowry, P. J. Nature 299, 355–357 (1982).

    Article  ADS  CAS  Google Scholar 

  2. Turkelson, C. M. et al. Peptides 3, 111–113 (1982).

    Article  CAS  Google Scholar 

  3. Vale, W. et al. Endocrinology 113, 1121–1131 (1983).

    Article  CAS  Google Scholar 

  4. Vale, W. W., Rivier, C., Spiess, J. & Rivier, J. in Brain Peptides (eds Krieger, D., Martin, J. B. & Brownstein, M. J.) 961–974 (Wiley, New York, 1983).

    Google Scholar 

  5. Gibbs, D. M. & Vale, W. Endocrinology 111, 1418–1420 (1982).

    Article  CAS  Google Scholar 

  6. Gibbs, D. M. & Vale, W. Brain Res. 280, 176–179 (1983).

    Article  CAS  Google Scholar 

  7. Plotsky, P. M., Bruhn, T. O. & Vale, W. Endocrinology 115, 1639–1641 (1984).

    Article  CAS  Google Scholar 

  8. Zimmerman, E. A. et al. Ann. N.Y. Acad. Sci. 297, 405–419 (1977).

    Article  ADS  CAS  Google Scholar 

  9. Yates, F. E. et al. Endocrinology 88, 2–15 (1971).

    Article  Google Scholar 

  10. Tramu, G., Croix, C. & Pillez, A. Neuroendocrinology 37, 467–469 (1983).

    Article  CAS  Google Scholar 

  11. Kiss, J. Z., Mezey, E. & Skirboll, L. Proc. natn. Acad. Sci. U.S.A. 81, 1854–1858 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Sawchenko, P. E., Swanson, L. W. & Vale, W. W. Proc. natn. Acad. Sci. U.S.A. 81, 1883–1887 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Tramu, G. & Pillez, A. C.r. hebd. Séanc. Acad. Sci., Paris Ser. III 294, 107–114 (1982).

    CAS  Google Scholar 

  14. Pelletier, G. et al. Neuroendocrinology 35, 402–404 (1982).

    Article  CAS  Google Scholar 

  15. Leranth, C., Antoni, F. A. & Palkovits, M. Regul. Peptides 6, 179–188 (1983).

    Article  CAS  Google Scholar 

  16. Liposits, Z. et al. Cell Tissue Res. 229, 191–196 (1983).

    Article  CAS  Google Scholar 

  17. Silverman, A. J. & Zimmerman, E. A. Cell Tissue Res. 159, 291–301 (1975).

    Article  CAS  Google Scholar 

  18. Dube, D., Leclerc, R. & Pelletier, G. Am. J. Anat. 147, 103–108 (1976).

    Article  CAS  Google Scholar 

  19. Newman, G. R., Jasani, B. & Williams, E. D. Histochem. J. 15, 543–555 (1983).

    Article  CAS  Google Scholar 

  20. Whitnall, M. H., Castel, M., Key, S. & Gainer, H. Peptides 6, 241–247 (1985).

    Article  CAS  Google Scholar 

  21. Bendayan, M. & Zollinger, M. J. Histochem. Cytochem. 31, 101–109 (1983).

    Article  CAS  Google Scholar 

  22. Sawchenko, P. E., Swanson, L. W. & Vale, W. W. J. Neurosci. 4, 1118–1129 (1984).

    Article  CAS  Google Scholar 

  23. Burlet, A., Tonon, M.-C., Tankosic, P., Coy, D. & Vaudry, H. Neuroendocrinology 37, 64–72 (1983).

    Article  CAS  Google Scholar 

  24. Dreyfuss, F., Burlet, A., Tonon, M.-C. & Vaudry, H. Neuroendocrinology 39, 284–287 (1984).

    Article  CAS  Google Scholar 

  25. Land, H., Schutz, G., Schmale, H. & Richter, D. Nature 295, 299–303 (1982).

    Article  ADS  CAS  Google Scholar 

  26. Castel, M., Gainer, H. & Dellman, H.-D. Int. Rev. Cytol. 88, 303–459 (1984).

    Article  CAS  Google Scholar 

  27. Ben-Barak, Y., Russell, J. T., Whitnall, M. H., Ozato, K. & Gainer, H. J. Neurosci. 5, 81–97 (1985).

    Article  CAS  Google Scholar 

  28. Whitnall, M. H., Key, S., Ben-Barak, Y., Ozato, K. & Gainer, H. J. Neurosci. 5, 98–109 (1985).

    Article  CAS  Google Scholar 

  29. Stillman, M. A. et al. Endocrinology 101, 42–49 (1977).

    Article  CAS  Google Scholar 

  30. Chateau, M., Burlet, A. & Marphetti, J. J. Physiol., Paris 68, 10B–11B (1974).

    Google Scholar 

  31. Vandesande, F., DeMey, J. & Dierickx, K. Cell Tissue Res. 151, 187–200 (1974).

    Article  CAS  Google Scholar 

  32. Watkins, W. B., Schwabedal, P. & Bock, R. Cell Tissue Res. 152, 411–421 (1974).

    Article  CAS  Google Scholar 

  33. Wolfson, B., Manning, R. W., Davis, L. G., Arentzen, R. & Baldino, F. Nature 315, 59–61 (1985).

    Article  ADS  CAS  Google Scholar 

  34. Russell, J. T., Brownstein, M. J. & Gainer, H. Brain Res. 201, 227–234 (1980).

    Article  CAS  Google Scholar 

  35. Holmes, M. C., Antoni, F. A. & Szentendrei, T. Neuroendocrinology 39, 162–169 (1984).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Mark H. Whitnall & Harold Gainer

  2. Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland, 20892, USA

    Ėva Mezey

Authors
  1. Mark H. Whitnall
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  2. Ėva Mezey
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  3. Harold Gainer
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Whitnall, M., Mezey, Ė. & Gainer, H. Co-localization of corticotropin-releasing factor and vasopressin in median eminence neurosecretory vesicles. Nature 317, 248–250 (1985). https://doi.org/10.1038/317248a0

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