Summary
The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80–120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Armstrong WE, Warach S, Hatton GI, McNeill TH (1980) Subnuclei in the rat hypothalamic paraventricular nucleus: A cytoarchitectural, horseradish peroxidase and immunocyto-chemical analysis. Neuroscience 5:1931–1958
Berod A, Hartman BK, Pujol JF (1981) Importance of fixation in immunohistochemistry: Use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase. J Histochem Cytochem 29:844–850
Bugnon C, Fellmann D, Gouget A, Cardot J (1982) Corticoliberin in the rat brain: Immunocy-tochemical localization of a novel neuroglandular system. Neurosci Lett 30:25–30
Merchenthaler I, Vigh S, Petrusz P, Schally AV (1982) Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat brain. Am J Anat (in press)
Palkovits M (1973) Isolated removal of hypothalamic and other brain nuclei of the rat brain. Brain Res 59:449–450
Spiess J, Rivier J, Rivier C, Vale W (1981) Primary structure of corticotropin-releasing factor from ovine hypothalamus. Proc Natl Acad Sci USA 78:6517–6521
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:346–354
Streit P, Reubi JC (1977) A new and sensitive staining method for axonally transported peroxidase (HRP) in the pigeon visual system. Brain Res 126:530–537
Tramu G, Pillez A (1982) Localisation immunohistochimique des terminaisons nerveuses á corticolibérine (CRF) dans l'éminence médiane du Cobaye et du Rat. CR Acad Sc Paris 294:107–114
Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin Science 213:1394–1397
Vigh S, Merchenthaler I, Petrusz P, Suerias-Diaz J, Schally AV (1982a) Specific antibodies against the corticotropin releasing factor (CRF); Immunocytochemical localization of CRF in the rat brain. The End Soc 64th Annual Meeting, San Francisco, p 84
Vigh S, Merchenthaler I, Torres-Alleman I, Sueriaz-Diaz J, Coy DV, Carter WH, Petrusz P, Schally AV (1982b) Corticotropin releasing factor (CRF): immunocytochemical localization and radioimmunoassay (RIA). Life Sci (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liposits, Z., Görcs, T., Sétáló, G. et al. Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain. Cell Tissue Res. 229, 191–196 (1983). https://doi.org/10.1007/BF00217891
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00217891