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Vanadate blocks cyclic AMP-induced Stimulation of sodium and water transport in amphibian epithelia

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Abstract

INTEREST in the biological effects of vanadium has grown particularly since Cantley et al.1 identified vanadate as a potent inhibitor of (Na+ + K+) ATPase and Balfour et al.2 reported its striking natriuretic and diuretic properties in the rat. Such findings prompted us to investigate the effects of vanadate on amphibian epithelia, a model widely used for studying, in vitro, the membrane transport of sodium and water3,4. We found a ouabain-like action of metavanadate on active sodium transport. In addition, we report here a new biological effect of this compound on living cells: the inhibition of cyclic AMP-induced stimulation of transepithelial water flow.

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References

  1. Cantley, L. C. Jr et al. J. biol. Chem. 252, 7421–7423 (1977).

    CAS  Google Scholar 

  2. Balfour, W. E., Grantham, J. J. & Glynn, I. M. Nature 275, 768 (1978).

    Article  ADS  CAS  Google Scholar 

  3. De Sousa, R. C. J. Physiol., Lond. 71, 5A–71A (1975).

    CAS  Google Scholar 

  4. Leaf, A. Rev. Physiol. 56, 215–263 (1965).

    Google Scholar 

  5. Li, J. H. & De Sousa, R. C. J. Membrane Biol. 46, 155–169 (1979).

    Article  CAS  Google Scholar 

  6. De Sousa, R. C. & Grosso, A. J. Membrane Biol. 40, 77–94 (1978).

    Article  CAS  Google Scholar 

  7. Flier, J. S. Nature 274, 285–286 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Cantley, L. C. Jr, Resh, M. D. & Guidotti, G. Nature 272, 552–554 (1978).

    Article  ADS  CAS  Google Scholar 

  9. Rüphi, M., De Sousa, R. C., Favrod-Coune, E. & Posternak, J. M. 28, 1391–1393 (1972).

  10. Grosso, A. & De Sousa, R. C. J. Membrane Biol. 40, Suppl. 305–321 (1978).

    Article  CAS  Google Scholar 

  11. Hackbarth, I. et al. Nature 275, 67 (1978).

    Article  ADS  CAS  Google Scholar 

  12. Josephson, L. & Cantley, L. C. Jr Biochemistry 16, 4572–4578 (1977).

    Article  CAS  Google Scholar 

  13. Beaugé, L. A. & Glynn, I. M. Nature 272, 551–552 (1978).

    Article  ADS  Google Scholar 

  14. Cantley, L. C. Jr, Cantley, L. G. & Josephson, L. J. biol. Chem. 253, 7361–7368 (1978).

    CAS  PubMed  Google Scholar 

  15. Schwabe, U., Puchstein, C., Hannemann, H. & Söchtig, E. Nature 277, 143–145 (1979).

    Article  ADS  CAS  Google Scholar 

  16. Mills, J. W. & Malick, L. E. J. Cell Biol. 77, 598–610 (1978).

    Article  CAS  Google Scholar 

  17. Taylor, A., Mamelak, M., Reaven, E. & Maffly, R. Science 181, 347–349 (1973).

    Article  ADS  CAS  Google Scholar 

  18. De Sousa, R. C., Grosso, A. & Rufener, C. Experientia 30, 175–177 (1974).

    Article  CAS  Google Scholar 

  19. Grosso, A., Spinelli, F. & De Sousa, R. C. Cell Tissue Res. 188, 375–388 (1978).

    Article  CAS  Google Scholar 

  20. Kobayashi, T., Martensen, T., Nath, J. & Flavin, M. Biochem. biophys. Res. Commun. 81, 1313–1318 (1978).

    Article  CAS  Google Scholar 

  21. Gibbons, I. R. et al. Proc. natn. Acad. Sci. U.S.A. 75, 2220–2224 (1978).

    Article  ADS  CAS  Google Scholar 

  22. Cande, W. Z. & Wolniak, S. M. J. Cell Biol. 79, 573–580 (1978).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Physiology and Medicine, School of Medicine, University of Geneva, 1211, Geneva, 4, Switzerland

    R. C. DE SOUSA & A. GROSSO

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  1. R. C. DE SOUSA
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  2. A. GROSSO
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DE SOUSA, R., GROSSO, A. Vanadate blocks cyclic AMP-induced Stimulation of sodium and water transport in amphibian epithelia. Nature 279, 803–804 (1979). https://doi.org/10.1038/279803a0

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