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Characterization of bacterial populations from Murciano-Granadina goat colostrum

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Dairy Science & Technology

Abstract

Bacterial populations present in goat colostrum samples were examined in order to determine the sanitary quality and the presence of potentially probiotic lactic acid bacteria. Both culture-dependent and culture-independent (PCR-denaturing gradient gel electrophoresis (DGGE)) methods were used. The results obtained from PCR-DGGE analysis demonstrated the presence of bacterial species belonging to the lactic acid bacteria group, including Lactococcus lactis, Lactobacillus delbrueckii, Lactobacillus fermentum and Pediococcus acidilactici, and others belonging to the Staphylococcus, Enterobacter and Escherichia genera. Enrichment cultures confirmed the presence of these bacterial groups, and in addition, growth on selective media for enterococci and streptococci was observed. It was concluded that goat colostrum has microbial characteristics comparable to those of human colostrum, being an important source of lactic acid bacteria and other related bacteria. It makes goat colostrum an interesting source of potentially probiotic bacteria, which will contribute to finding new potential uses for this product and will benefit livestock farms.

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Acknowledgments

This research has been financed by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Acción Estratégica “Conservación de recursos genéticos de interés agroalimentario” del Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Ciencia e Innovación, Project number RM 2010-00010-00-00. Patricia Ruiz was contracted by a charge to the same project.

Conflict of interest

Patricia Ruiz, Susana Seseña, and María Llanos Palop declare that they have no conflict of interest.

Compliance with Ethics Guidelines

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Department of Analytical Chemistry and Food Technology, Environmental Sciences and Biochemistry Faculty, University of Castilla-La Mancha, Avda. Carlos III, s/n, 45071, Toledo, Spain

    Patricia Ruiz, Susana Seseña & María Llanos Palop

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  1. Patricia Ruiz
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  2. Susana Seseña
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  3. María Llanos Palop
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Correspondence to María Llanos Palop.

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Ruiz, P., Seseña, S. & Palop, M.L. Characterization of bacterial populations from Murciano-Granadina goat colostrum. Dairy Sci. & Technol. 94, 549–560 (2014). https://doi.org/10.1007/s13594-014-0179-0

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  • DOI: https://doi.org/10.1007/s13594-014-0179-0

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