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Analysis of promoter activity of PtDrl02 gene in white poplars

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Abstract

To determine the transcriptional activity of the promoter of Populus TIR (Toll/interleukin-1 receptor domain)-encoding PtDrl02 gene in perennial plants, β-glucuronidase (GUS) gene expression driven by PtDrl02 promoter was analyzed in white poplars. Agrobacterium-mediated transient expression assays indicated that the PtDrl02 promoter was able to direct the GUS reporter gene transcription in stem tissues of both triploid [(Populus tomentosa × P. bolleana) × P. tomentosa, clone ‘L9’] and diploid (P. tomentosa cv. 1521) white poplars, and that the transcript levels seemed to be comparable to each other. In stably transformed P. tomentosa plants, the PtDrl02 promoter directed-gene (GUS) expression behaved in an aerial-specific manner but with a relatively low level in tissues compared to that of the ACTIN reference. Further investigation revealed that the PtDrl02 promoter activity could be induced by wounding, methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), or high salinity (NaCl) in transgenic P. tomentosa but with a time-course manner. Intriguingly, dynamic expression of the GUS reporter gene driven by PtDrl02 promoter, over given time periods, appeared to be similar to that of the native pathogenesis-related (PR) protein gene (PtPR-1, PtPR-5, or PtPR-10) in transgenic P. tomentosa plants when exposed to the same inducers. Here it was also evidenced that the PtWRYK1 transcription factor conferred a negative effect on PtDrl02 promoter functions in white poplars. Our work provided new information on PtDrl02 promoter activity in poplars, which could be helpful to increase our understanding of the transcription regulation of the Populus TIR-encoding gene by its promoter.

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Abbreviations

GUS:

β-glucuronidase

LRR:

Leucine rich repeat

NBS:

Nucleotide binding site

PR:

Pathogenesis-related

TIR:

Toll/interleukin-1 receptor domain

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Acknowledgements

This research was supported by the National Natural Sciences Foundation of China (No. 30872043), the National ‘863’ Program in China (No.2009AA10Z107) and the National ‘948’ Plan Project in China (No. 2006-4-72).

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Author notes

  1. Huiquan Zheng & Qian Zhang

    Present address: Guangdong Academy of Forestry, 510520, Guangzhou, People’s Republic of China

Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, NDRC; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE; Laboratory of Tree and Ornamental Plant Breeding and Biotechnology, SFA, Beijing Forestry University, 100083, Beijing, People’s Republic of China

    Huiquan Zheng, Yang Lei, Zhiyi Zhang, Shanzhi Lin, Qian Zhang, Wenfeng Liu, Juan Du, Xinmin An & Xiyang Zhao

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  1. Huiquan Zheng
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Correspondence to Zhiyi Zhang.

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Huiquan Zheng and Yang Lei contributed equally to this work.

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Zheng, H., Lei, Y., Zhang, Z. et al. Analysis of promoter activity of PtDrl02 gene in white poplars. J. Plant Biochem. Biotechnol. 21, 88–97 (2012). https://doi.org/10.1007/s13562-011-0084-z

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