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New insights into the evolutionary history of resistance gene candidates in coconut palms and their expression profiles in palms affected by lethal yellowing disease

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Abstract

The nucleotide binding site and leucine rich repeat (NBS–LRR) class of R genes is the most comprehensively studied in terms of sequence evolution; however, in coconut palm and, more generally, in the family of Arecaceae, our understanding of the evolution of these genes is rather limited. In this study, disease resistance gene candidates (RGCs) of the nucleotide binding site (NBS) type of coconut palm were used to investigate evolutionary relationships in Arecaceae, Poaceae and Brassicaceae species. The results indicate a species-specific evolution of RGCs in coconut palm. However, strikingly similar RGCs between species of Arecales indicate a high conservation of specific RGCs of this family, suggesting a monophyletic origin of three genera. The phylogenetic relationship between RGCs of Arecales and Brassicales suggests that these sequences possibly emerged before being divided between monocots and dicots. Finally the comparative analysis of the expression of four RGCs in healthy coconut palm and those affected with lethal yellowing disease revealed differences in their expression profiles. This study provides new insights for future efforts towards the improvement of disease resistance in coconut palm and other species of Arecaceae.

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Acknowledgments

Funding of the research reported here was provided by CONACyT, México (Grant No. CB 129717). Carlos Puch-Hau and Manuel Gongora thank CONACyT for the scholarship awarded No. 47245 and 265354, respectively.

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Authors and Affiliations

  1. Biotechnology Unit, Centro de Investigación Científica de Yucatán, Calle 43 No. 130. Colonia Chuburna de Hidalgo, 97200, Mérida, YUC, Mexico

    Carlos Puch-Hau, Carlos Oropeza, Manuel Góngora-Paredes, Iván Córdova & Luis Sáenz

  2. Instituto Tecnológico de Conkal, Mérida, YUC, Mexico

    José Tun-Suárez

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  1. Carlos Puch-Hau
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  2. Carlos Oropeza
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  3. Manuel Góngora-Paredes
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  4. Iván Córdova
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  5. José Tun-Suárez
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  6. Luis Sáenz
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Correspondence to Luis Sáenz.

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Puch-Hau, C., Oropeza, C., Góngora-Paredes, M. et al. New insights into the evolutionary history of resistance gene candidates in coconut palms and their expression profiles in palms affected by lethal yellowing disease. Genes Genom 38, 793–807 (2016). https://doi.org/10.1007/s13258-016-0422-6

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  • DOI: https://doi.org/10.1007/s13258-016-0422-6

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