Abstract
The basic leucine zipper (bZIP) proteins are ubiquitous in plants and play important roles in plant defense responses. In this study, based on an expressed sequence tag from a suppression subtractive hybridization cDNA library of Lilium regale Wilson during Fusarium oxysporum f. sp. lilii infection, a novel bZIP transcription factor gene LrbZIP1 was isolated from L. regale root using the rapid amplification of cDNA ends method. The predicted protein of LrbZIP1 with 142 amino acid residues contains a basic domain signature and a leucine zipper motif. The quantitative reverse transcription-PCR (qRT-PCR) analysis showed that the transcription level of LrbZIP1 was higher in roots of L. regale than in young stems and leaves. Moreover, the expression of LrbZIP1 was up-regulated in the incompatible interaction between L. regale and F. oxysporum f. sp. lilii as well as after treatments with stress-related signaling molecules. To verify the function of LrbZIP1, a constitutive expression vector of LrbZIP1 was constructed and transferred into tobacco (Nicotiana tabacum L. cv Xanthi). The results of Southern blotting and qRT-PCR analyses demonstrated that the LrbZIP1 was integrated into genome of the tobacco transformants and highly expressed. Under normal conditions, the T1 transgenic tobacco lines showed higher antioxidant enzyme activities and transcription levels of several resistance-related genes than the wild type. Moreover, the T1 transgenic tobacco plants showed strong resistance to F. oxysporum f. sp. lilii infection.
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References
Alves MS, Dadalto SP, Gonçalves AB, De Souza GB, Barros VA, Fietto LG (2013) Plant bZIP transcription factors responsive to pathogens: a review. Int J Mol Sci 14:7815–7828
Chisholm ST, Coaker G, Day B, Staskawicz BJ (2006) Host-microbe interactions: shaping the evolution of the plant immune response. Cell 124:803–814
Gay PA, Tuzun S (2000) Temporal and spatial assessment of defense responses in resistant and susceptible cabbage varieties during infection with Xanthomonas campestris pv. campestris. Physiol Mol Plant P 57:201–210
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
Jakoby M, Wesisshaar B, Dröge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F (2002) bZIP transcription factors in Arabidopsis. Trends Plant Sci 7:106–111
Jayaraj J, Punja ZK (2007) Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens. Plant Cell Rep 26:1539–1546
Johnson C, Boden E, Arisa J (2003) Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis. Plant Cell 15:1846–1858
Jones JDG, Dangl JL (2006) The plant immune system. Nature 444:323–329
Kim HS, Delaney TP (2002) Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. Plant J 32:151–163
Kogel KH, Langen G (2005) Induced disease resistance and gene expression in cereals. Cell Microbiol 7:1555–1564
Kotchoni SO, Gachomo EW (2006) The reactive oxygen species network pathways: an essential prerequisite for perception of pathogen attack and the acquired disease resistance in plants. J Biosci 31:389–404
Lee SJ, Lee MY, Yi SY, Oh SK, Choi SH, Her NH, Choi D, Her NH, Choi D, Min BW, Yang SG, Harn CH (2002) PPI1: a novel pathogen-induced basic region-leucine zipper (bZIP) transcription factor from pepper. Mol Plant Microbe Interact 15:540–548
Lee SC, Choi HW, Hwang IS, Hwang BK (2006) Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta 224:1209–1225
Lim JH, Rhee HK, Kim YJ, Lim KB, van Tuyl JM (2003) Resistance to Fusarium oxysporum f. sp. lilii in Lilium. Acta Hort 620:311–318
Liu D, Zhang X, Tu L, Zhu L, Guo X (2006) Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development stages in cotton. Mol Biol 40:825–834
Liu D, Li W, He X, Ding Y, Chen C, Ge F (2013) Characterization and functional analysis of a novel PGIP gene from Pyrus pyrifolia Nakai cv Huobali. Acta Physiol Plant 35:1247–1256
Meng XB, Zhao WS, Lin RM, Wang M, Peng YL (2005) Identification of a novel rice bZIP-type transcription factor gene, OsbZIP1, involved in response to infection of Magnaporthe grisea. Plant Mol Biol Rep 23:301–302
Munis MF, Tu L, Deng F, Tan J, Xu L, Xu S, Long L, Zhang X (2010) A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco. Biochem Biophys Res Commun 393:38–44
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136
Rao J, Liu D, Zhang N, He H, Ge F, Chen C (2013) Identification of genes differentially expressed in a resistant reaction to Fusarium oxysporum in Lilium regale by SSH. IERI Procedia 5:95–101
Tak H, Mhatre M (2013) Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera. Protoplasma 250:333–345
Vettore AL, Yunes JA, Cord Neto G, da Silva MJ, Arruda P, Leite A (1998) The molecular and functional characterization of an Opaque2 homologue gene from Coix and a new classification of plant bZIP proteins. Plant Mol Biol 36:249–263
Yáñez M, Cáceres S, Orellana S, Bastías A, Verdugo I, Ruiz-Lara S, Casaretto JA (2009) An abiotic stress-responsive bZIP transcription factor from wild and cultivated tomatoes regulates stress-related genes. Plant Cell Rep 28:1497–1507
Zhang Y, Zhang G, Xia N, Wang XJ, Huang LL, Kang ZS (2009) Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses. Physiol Mol Plant P 73:88–94
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This work was financially supported by grant from the National Natural Sciences Foundation of China (31160401).
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13258_2014_214_MOESM1_ESM.tif
Fig. S1. Schematic diagram of the expression construct containing Lilium regale’s novel bZIP transcription factor gene, LrbZIP1 The LrbZIP1 gene was under control of the CaMV 35S promoter and linked to the kanamycin resistance gene NPTII
13258_2014_214_MOESM2_ESM.tif
Fig. S2. Plant morphology of wild type (WT) and five LrbZIP1 transgenic tobacco lines under normal conditions There was no visible difference between the positive transgenic plants and WT
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Zhang, N., Liu, D., Zheng, W. et al. A bZIP transcription factor, LrbZIP1, is involved in Lilium regale Wilson defense responses against Fusarium oxysporum f. sp. lilii . Genes Genom 36, 789–798 (2014). https://doi.org/10.1007/s13258-014-0214-9
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DOI: https://doi.org/10.1007/s13258-014-0214-9