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Partial Purification and Characterization of a Bacteriocin DT24 Produced by Probiotic Vaginal Lactobacillus brevis DT24 and Determination of its Anti-Uropathogenic Escherichia coli Potential

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Abstract

The emergence of antibiotic resistance has increased the interest for finding new antimicrobials in the past decade. Probiotic Lactic acid bacteria producing antimicrobial proteins like bacteriocin can be excellent agents for development as novel therapeutic agents and complement to conventional antibiotic therapy. Uropathogenic Escherichia coli, most causative agent of Urinary tract infection, has developed resistance to various antibiotics. In the present investigation, antibacterial substance like bacteriocin (Bacteriocin DT24) produced by probiotic Lactobacillus brevis DT24 from vaginal sample of healthy Indian woman was partially purified and characterized. It was efficiently working against various pathogens, that is, Uropathogenic E. coli, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The antimicrobial peptide was relatively heat resistant and also active over a broad range of pH 2–10. It has been partially purified by ammonium sulfate precipitation and gel filtration chromatography and checked on reverse-phase high-performance liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacteriocin DT24 was approximately 7-kDa protein. The peptide is inactivated by proteolytic enzymes, trypsin and lipase but not when treated with catalase, α-amylase and pepsin. It showed bacteriostatic mode of action against uropathogenic E. coli. Such characteristics indicate that this bacteriocin-producing probiotic may be a potential candidate for alternative agents to control urinary tract infections and other pathogens.

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Acknowledgments

The authors are thankful to Nirma Education and Research Foundation (NERF), Ahmedabad for providing infrastructure and financial support.

Conflict of interest

The author(s) herewith certifies that there is no financial or commercial or proprietary interest in the products or companies described in the manuscript. There is no Conflict of Interest among the authors.

Ethical Standard

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation as per Institutional ethical committee, Nirma University (Ahmedabad). Protocol No. IEC/NU/I/IS/03 and with the Helsinki Declaration of 1975, as revised in 2008 (5). Informed consent was obtained from all patients for being included in the study. Additional informed consent was obtained from all patients for which identifying information is included in this article. This article does not contain any studies with animal subjects.

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  1. Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Chharodi, Ahmedabad, 382481, Gujarat, India

    Disha Trivedi, Prasant kumar Jena, Jignesh kumar Patel & Sriram Seshadri

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  1. Disha Trivedi
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  2. Prasant kumar Jena
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  3. Jignesh kumar Patel
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Correspondence to Sriram Seshadri.

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Trivedi, D., Jena, P.k., Patel, J.k. et al. Partial Purification and Characterization of a Bacteriocin DT24 Produced by Probiotic Vaginal Lactobacillus brevis DT24 and Determination of its Anti-Uropathogenic Escherichia coli Potential. Probiotics & Antimicro. Prot. 5, 142–151 (2013). https://doi.org/10.1007/s12602-013-9132-4

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