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Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi

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Abstract

Growth hormone receptors (GHRs) are especially expressed in the muscle and liver tissues of mandarin fish Siniperca chuatsi. cDNAs of two GHR types (scGHR1 and scGHR2: 1999 and 1963 bp, encoding 638 and 578 amino acids, respectively) have been isolated and characterized in mandarin fish. Both receptors, formed through fish-specific genome duplication, have conserved FGEFS and box1 and 2 motifs. Partial compensatory growth was noted following 10-day re-feeding after 4-week fasting. After 1-week fasting, pituitary GH and muscle scGHR1 and scGHR2 mRNA levels and plasma GH concentrations increased (p < 0.05), while hepatic scGHR1 and scGHR2 mRNA expressions reduced rapidly (p < 0.05). Upon re-feeding, muscle scGHR1 and scGHR2 mRNA expressions decreased, whereas they increased rapidly in the liver, eventually normalizing. Pituitary GH mRNA expression increased to 5.1 times higher than that in the controls 3 days after re-feeding (p < 0.01), and normalized 7 days after re-feeding. The varying expression of scGHR1 and scGHR2 in different tissues suggests their distinct functions. Further, changes in GH, scGHR1, and scGHR2 mRNA expressions suggest their important roles in the growth of mandarin fish. Our results provide comparative insights into evolutionary origins and roles of GHR genes in teleost growth.

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Acknowledgments

This work was supported by the Educational Commission of Guangdong Province, China (No. cxzd1104), the Foshan Innovative and Entepreneurial Research Team Program (No. 2014IT100122), the Special Fund for Agro-scientific Research in the Public Interest (No. 201303048), the Science and Technology Planning Project of Guangdong Province (No. 2007A020300001-1; No. 2012A020800001; No. 2008A020100003), the Cooperative Project of Guangdong Province (No. 2011B090400179) and the Agriculture Science Technology Achievement Transformation project (No.2 012GB2E000338).

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Authors and Affiliations

  1. Key Laboratory of Aquatic Product Safety (Ministry of Education), State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, No. 135 Xingang Xi Road, Guangzhou, 510275, People’s Republic of China

    Xue Lu, Xiaocui Hou, Haifang Wang, Pengfei Wang, Peng Xu, Lei Zeng, Lei Zhou & Guifeng Li

  2. Foshan Nanhai Holdone Aquatic Seeds Co., Ltd, Foshan, 528216, People’s Republic of China

    Yongming Gu

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  1. Xue Lu
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  3. Xiaocui Hou
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Lu, X., Gu, Y., Hou, X. et al. Molecular characterization, tissue distribution, and expression regulation from fasting and re-feeding of two growth hormone receptors in mandarin fish Siniperca chuatsi . Fish Sci 82, 155–169 (2016). https://doi.org/10.1007/s12562-015-0941-0

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