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Identification of insulin-like growth factor I gene polymorphisms using high-resolution melting and its effect on growth traits in sinipercid species

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  • Aquaculture
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Abstract

The aim of this study is to screen single nucleotide polymorphisms (SNPs) of the insulin-like growth factor I (IGF-I) gene and analyze the potential association between IGF-I gene polymorphisms and growth traits in a crossing sinipercid population. Two SNPs (g.A321G, g.A537G) within five exons of the IGF-I gene were tested for association with four growth traits in 250 individuals using the high-resolution melting method. The association analysis of SNPs of IGF-I gene with the four growth traits was carried out using General Linear Model estimation. Results indicated that SNP1 in exon3 of the IGF-I gene was significantly associated with body weight (P < 0.05), body length (P < 0.05) and body width (P < 0.05), and SNP2 in exon5 of the IGF-I gene was significantly associated with body weight (P < 0.05), body depth (P < 0.05) and body width (P < 0.05) in a sinipercid population. The individuals of genotype AA or AG in loci SNP1 and SNP2 grew faster than those of genotype GG. Diplotypes did not show significant effects on growth traits. These findings implied that the two SNPs of the IGF-I gene affecting growth traits could be potential quantitative trait nucleotides and would be useful genetic markers in the selection of some growth traits for developing improved culture lines of sinipercid species in China.

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References

  1. Moriyama S, Ayson FG, Kawauchi H (2000) Growth regulation by insulin-like growth factor-I in fish. Biosci Biotech Biochem 64:1553–1562

    Article  CAS  Google Scholar 

  2. Scatà MC, Catillo G, Annicchiarico G, Matteis GD, Napolitano F, Signorelli F, Moioli B (2010) Investigation on lactation persistency and IGF-I gene polymorphisms in dairy sheep. Small Rumin Res 89:7–11

    Article  Google Scholar 

  3. Faria DA, Peixoto JD, Lopes PS, Paiva SR, Silva PV, Guimaraes SE, (2009) Association between insulin-like growth factor I (IGF-I) microsatellite polymorphisms and important economic traits in pigs. Revista brasileira de zootechia-brazilian J Anim Sci 38:265–270

    Google Scholar 

  4. Tahmoorespur M, Valeh MV, Nassiry MR, Moussavi AH, Ansary M (2009) Association of the polymorphism in the 5’ flanking region of the ovine IGF-I gene with growth traits in the Baluchi sheep. S Afr J Anim Sci 39:97–101

    Google Scholar 

  5. Bonakdar E, Rahmani HR, Edriss MA, Tabatabaei BES (2010) IGF-I gene polymorphism, but not its blood concentration, is associated with milk fat and protein in Holstein dairy cows. Genet Mol Res 9:1726–1734

    Article  PubMed  CAS  Google Scholar 

  6. Guo CH, Jia CL, Zhang W, Zhu XP, Jia ZH (2009) Polymorphisms in the 5’ flanking region of IGF- I gene are associated with cashmere fibre traits in liaoning cashmere goats. Prog Biochem Biophys 36:566–573

    Article  CAS  Google Scholar 

  7. Deng CJ, Ma RN, Yue XP, Lan XY, Chen H, Lei CZ (2010) Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats. Genet Mol Biol 33:266–270

    Article  PubMed  CAS  Google Scholar 

  8. Li HF, Zhu WQ, Chen KW, Wu X, Tang QP, Gao YS, Song WT, Xu WJ, Xu HL (2009) Polymorphism in NPY and IGF-I genes associate with reproductive traits in Wenchang chicken. Afr J Biotechnol 8:4744–4748

    CAS  Google Scholar 

  9. Dyer AR, Barlow CG, Bransden MP, Carter CG, Glencross BD, Richardson N, Thomas PM, Williams KC, Carragher JF (2004) Correlation of plasma IGF-I concentrations and growth rate in aquacultured finfish: a tool for assessing the potential of new diets. Aquaculture 236:583–592

    Article  CAS  Google Scholar 

  10. Li XH, Bai JJ, Ye X, Hu YC, Li SJ, Yu LY (2009) Polymorphisms in the 5′ flanking region of the insulin-like growth factor I gene are associated with growth traits in largemouth bass Micropterus salmoides. Fish Sci 75:351–358

    Article  CAS  Google Scholar 

  11. Liu J, Zhao JL, Zhang M, Dai W (2011) Full-length cDNA cloning and tissue expression of insulin-like growth factor-I in the mandarin fish, Siniperca chuatsi. Chinese J Zool 46:28–36

    CAS  Google Scholar 

  12. Garritano S, Gemignani F, Voegele C, Nguyen DT, Le CKF, De SD, Lesueur F, Landi S, Tavtigian SV (2009) Determining the effectiveness of high resolution melting analysis for SNP genotyping and mutation scanning at the TP53 locus. BMC Genet 10:5

    Article  PubMed  Google Scholar 

  13. Martino A, Mancuso T, Rossi AM (2010) Application of high-resolution melting to large-scale, high-throughput SNP genotyping: a comparison with the TaqMan® method. J Biomol Screen 15:623–629

    Article  PubMed  CAS  Google Scholar 

  14. Seipp MT, Pattison D, Durtschi JD, Jama M, Voelkerding KV, Wittwer CT (2008) Quadruplex genotyping of F5 F5, F2 F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting. Clin Chem 54:108–115

    Article  PubMed  CAS  Google Scholar 

  15. Joly P, Lacan P, Garcia C, Delasaux A, Francina A (2011) Rapid and reliable β-globin gene cluster haplotyping of sickle cell disease patients by FRET Light Cycler and HRM assays. Clin Chim Acta 412:1257–1261

    Article  PubMed  CAS  Google Scholar 

  16. Haynes GD, Gongora J, Nicholas FW, Zenger KR (2009) Rapid identification of maternal lineages in common carp Cyprinus carpio L. using real-time PCR and high resolution melt-curve analysis. Aquaculture 287:59–66

    Article  CAS  Google Scholar 

  17. Ishikawa T, Kamei Y, Otozai S, Kim J, Sato A, Kuwahara Y, Tanaka M, Deguchi T, Inohara H, Tsujimura T, Todo T (2010) High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library. BMC Mol Biol 11:70

    Article  PubMed  Google Scholar 

  18. Senapin S, Molthathong S, Phiwsaiya K, Jaengsanong C, Chuchird N (2010) Application of high resolution melt (HRM) analysis for duplex detection of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in shrimp. Mol Cell Probe 24:291–297

    Article  CAS  Google Scholar 

  19. Liu HZ, Chen YY (1994) Phylogeny of the sinipercine fishes with some taxonomic notes. Zool Res 15:1–12

    Google Scholar 

  20. Zhou CW, Yang Q, Cai DL (1988) On the classification and distribution of the sinipercinae fishes (family Serranidae). Zool Res 9:113–125 (in Chinese with English abstract)

    Google Scholar 

  21. Zhao J, Zhu XP, Chen YL, Liu YH, Xie G (2008) Morphological variations of Siniperca chuatsi, Sniperca scherzeri and their hybrid S. chuatsi × S. scherzeri. J Huazhong Agric Univ 27:506–509 (in Chinese with English abstract)

    Google Scholar 

  22. Mi GQ, Lian QP, Wang YC, Shen TS (2009) A study on embryonic development of hybrid F1 by Siniperca chuatsi (♀) × Siniperca scherzeri (♂). J Shanghai Ocean Univ 18:421–427 (in Chinese with English abstract)

    Google Scholar 

  23. Mi GQ, Lian QP, Wang YC, Shen TS (2009) Observation on embryonic development of crossbreed F1 by Siniperca scherzeri (♀) × Siniperca chuatsi (♂). J Zhejiang Ocean Univ (Natural Science Edition) 28:264–269 (in Chinese with English abstract)

    Google Scholar 

  24. Zhao JL, Li CH, Zhao LL, Wang WW, Cao Y (2008) Mitochondrial diversity and phylogeography of the Chinese perch Siniperca chuatsi (Perciformes: Sinipercidae). Mol Phylogenet Evol 49:399–404

    Article  PubMed  CAS  Google Scholar 

  25. Thanh NM, Barnes AC, Mather PB, Li YT, Lyons RE (2010) Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture 301:7–15

    Article  Google Scholar 

  26. Weller JI (1994) Economic aspects of animal breeding. Chapman and Hall, UK

    Google Scholar 

  27. Gibson G, Muse SV (2004) A primer of genome science. Sinauer Associates, Sunderland, p 378

    Google Scholar 

  28. Tao WJ, Boulding EG (2003) Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr Salvelinus alpinus L. Heredity 91:60–69

    Article  PubMed  CAS  Google Scholar 

  29. Glenn KL, Grapes L, Suwanasopee T, Harris DL, Li Y, Wilson K, Rothschild MF (2005) SNP analysis of AMY2 and CTSL genes in Lito penaeus vannamei and Penaeus monodon shrimp. Anim Genet 36:235–236

    Article  PubMed  CAS  Google Scholar 

  30. Xu YX, Zhu ZY, Lo LC, Wang CM, Lin G, Feng F, Yue GH (2006) Characterization of two parvalbumin genes and their association with growth traits in Asian seabass Lates calcarifer. Anim Genet 37:266–268

    Article  PubMed  CAS  Google Scholar 

  31. Jiang GZ (2008) On the development of Chinese freshwater fish culture technology in the 20th century. China Doctoral Dissertations Full-text Database, pp 68

  32. Boer M, Hartl D, Wintergerst U, Belohradsky BH, Roos D (2005) A donor splice site mutation in intron 1 of CYBA, leading to chronic granulomatous disease. Blood Cell Mol Dis 35:365–369

    Article  Google Scholar 

  33. Hull J, Campino S, Rowlands K, Chan MS, Copley RR, Taylor MS, Rockett K, Elvidge G, Keating B, Knight J, Kwiatkowski D (2007) Identification of common genetic variation that modulates alternative splicing. PLoS Genet 3:1009–1018

    Article  CAS  Google Scholar 

  34. Canzian F, McKay JD, Cleveland RJ, Dossus L, Biessy C, Rinaldi S, Landi S, Boillot C, Monnier S, Chajes V et al (2006) Polymorphisms of genes coding for insulin-like growth factor 1 and its major binding proteins, circulating levels of IGF-I and IGFBP-3 and breast cancer risk: results from the EPIC study. Br J Cancer 94:299–307

    Article  PubMed  CAS  Google Scholar 

  35. Prudence M, Moal J, Boudry P, Daniel JY, Quere C, Jeffroy F, Mingant C, Ropert M, Bedier E, Wormhoudt AV, Samain JF, Huvet A (2006) An amylase gene polymorphism is associated with growth differences in the Pacific cupped oyster Crassostrea gigas. Anim Genet 37:348–351

    Article  PubMed  CAS  Google Scholar 

  36. He F, Wen HS, Dong SL, Shi B, Chen CF, Wang LS, Yao J, Mu XJ, Zhou YG (2008) Identification of estrogen receptor a gene polymorphisms by SSCP and its effect on reproductive traits in Japanese flounder (Paralichthys olivaceus). Comp Biochem Phys B 150:278–283

    Article  Google Scholar 

  37. He F, Wen HS, Dong SL, Shi B, Chen CF, Wang LS, Yao J, Mu XJ, Zhou YG (2008) Identification of single nucleotide polymorphism cytochrome P450-c19a and its relation to reproductive traits in Japanese flounder Paralichthys olivaceus. Aquaculture 279:177–181

    Article  CAS  Google Scholar 

  38. Li XH, Bai JJ, Ye X, Hu YC, Li SJ, Yu LY (2012) Genotypes, haplotypes and diplotypes of IGF-II SNPs and their association with growth traits in largemouth bass Micropterus salmoides. Mol Biol Rep 39:4359–4365

    Article  PubMed  CAS  Google Scholar 

  39. Qian J, Lin J, Yao DM, Chen Q, Xiao GF, Ji RB, Li Y, Yang J, Qian Z (2010) Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform. Clin Chim Acta 411:2097–2100

    Article  PubMed  CAS  Google Scholar 

  40. Beuzen ND, Stear MJ, Chang KC (2000) Molecular markers and their use in animal breeding. Vet J 160:42–52

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors are grateful for technical assistance and instrument support from the Research Platform of the College of Life Sciences, Sun Yat-sen University. The comments of Liu Lingzhi and Luo Du were greatly appreciated in revision of the manuscript. This research was supported by a project of the National Key Technology R&D Program, China (No. 2012BAD25B04), a project of the Science and Technology Planning Project of Guangdong Province, China (No. 2012A020800001, No. 2008A020100003, No. 2007A020300001-1), a project of the Educational Commission of Guangdong Province, China (No. cxzd1104), and a Cooperative Project of Guangdong Province, China (No. 2011B090400179).

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Authors and Affiliations

  1. MOE Key Laboratory of Aquatic Product Safety, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China

    Haifang Wang, Xue Lu, Pengfei Wang, Peng Xu, Lei Zeng & Guifeng Li

  2. College of Animal Science, South China Agricultural University, Guangzhou, 510642, China

    Jijia Sun

  3. Pearl River Fisheries Research Institution, Guangzhou, 510380, China

    Deguang Yu

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  1. Haifang Wang
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Correspondence to Guifeng Li.

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Wang, H., Sun, J., Lu, X. et al. Identification of insulin-like growth factor I gene polymorphisms using high-resolution melting and its effect on growth traits in sinipercid species. Fish Sci 79, 439–446 (2013). https://doi.org/10.1007/s12562-013-0607-8

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  • DOI: https://doi.org/10.1007/s12562-013-0607-8

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