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Novel polymorphisms of goat growth hormone and growth hormone receptor genes and their effects on growth traits

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Abstract

In this study, the polymorphisms of growth hormone (GH) gene 5′ promoter region and intron 8, exons 4 and 10 of growth hormone receptor (GHR) gene were analyzed in Xinong Saanen goats (SG) and Boer goats (BG). Two alleles (A and B) and three genotypes (AA, AB and BB) were detected at P1 locus of GH gene, and two alleles (G and T) and two genotypes (GG and GT) were detected at P4 locus of GHR gene by PCR-SSCP analysis. In addition, two single nucleotide polymorphisms (SNPs)-A73C (P1 locus) and G114T (P4 locus), were identified by DNA sequencing. The frequencies of alleles A and B in the two goat breeds were 0.61–0.62, and 0.39–0.38, respectively, and the frequencies of alleles G and T in the two goat breeds were 0.82–0.86, and 0.18–0.14, respectively. The SNP loci were in Hardy–Weinberg disequilibrium in both goat breeds (P < 0.05). Polymorphisms of GH and GHR genes were shown to be associated with growth traits in BG breed. AA and GG genotypes were associated with superior growth traits in 1-, 2- and 3-month old individuals. Hence, AA and GG genotypes are suggested to be a molecular marker for superior growth traits in BG breed.

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Acknowledgments

This study was supported by the National 863 Program of China (2007AA10Z167) and the “13115” Sci-Tech Innovation Programme of Shaanxi Province (2009ZDKG-19).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People’s Republic of China

    Xiaopeng An, Lixin Wang, Jinxing Hou, Guang Li, Yuxuan Song, Jiangang Wang, Mingming Yang, Yihong Cui & Binyun Cao

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  1. Xiaopeng An
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  2. Lixin Wang
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  3. Jinxing Hou
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  4. Guang Li
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Correspondence to Binyun Cao.

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Xiaopeng An, Lixin Wang, Jinxing Hou, Guang Li and Yuxuan Song equally contributed to this article.

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Table S1

Primer sequences and information of GH (P1-P2) and GHR (P3-P5) genes (DOC 45 kb)

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An, X., Wang, L., Hou, J. et al. Novel polymorphisms of goat growth hormone and growth hormone receptor genes and their effects on growth traits. Mol Biol Rep 38, 4037–4043 (2011). https://doi.org/10.1007/s11033-010-0522-3

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