Skip to main content
SpringerLink
Log in

Characterization of OsDREB6 responsive to osmotic and cold stresses in rice

  • Original Article
  • Published:
Journal of Plant Biology Aims and scope Submit manuscript

Abstract

We identified a new dehydration-responsive element-binding protein gene, OsDREB6, which is an A-6 subgroup member of the DREB subfamily, of AP2/EREBP proteins, in Oryza sativa ssp. Japonica cv. Nipponbare. Expression pattern analysis indicated that OsDREB6 was induced in response to dehydration, high salinity, and most importantly by cold stress (4°C). Analysis using the yeast one-hybrid system showed that OsDREB6 had transcriptional activation activity and bound specifically to the DRE ciselement. Overexpression of OsDREB6 increased tolerance to osmotic, high salt, and cold stresses in transgenic rice whereas, OsDREB6 double-stranded RNA interference (RNAi) lines were found to be more sensitive to these stresses than wild-type rice. Additionally, sugar, proline and catalase levels in OsDREB6 RNAi lines were significantly lower than in the corresponding OsDREB6 overexpression lines and control lines after PEG treatment. In contrast, malondialdehyde (MDA) levels were significantly higher in RNAi lines than in control and overexpression lines. Furthermore, expression levels of some stress-related genes were altered in the transgenic plants. The data suggests that OsDREB6 may participate in the stress response by mediating the expression of specific genes, and may also be involved plant metabolism. Together, these results indicated that OsDREB6 plays an important role in the response of plants to a wide range of osmotic stresses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Apse MP, Aharon GS, Snedden WA, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285:1256–1258

    Article  CAS  PubMed  Google Scholar 

  • Arbona V, Manzi M, Ollas Cd, Gómez-Cadenas A (2013) Metabolomics as a tool to investigate abiotic stress tolerance in plants. Int J Mol Sci 14:4885–4911

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Baker SS, Wilhelm KS, Thomashow MF (1994) The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought-and ABA-regulated gene expression. Plant Mol biol 24:701–713

    Article  CAS  PubMed  Google Scholar 

  • Bouaziz D, Pirrello J, Ben Amor H, Hammami A, Charfeddine M, Dhieb A, Bouzayen M, Gargouri-Bouzid R (2012) Ectopic expression of dehydration responsive element binding proteins (StDREB2) confers higher tolerance to salt stress in potato. Plant Physiol Bioch 60:98–108

    Article  CAS  Google Scholar 

  • Chen M, Wang QY, Cheng XG, Xu ZS, Li LC, Ye XG, Xia LQ, Ma YZ (2007) GmDREB2, a soybean DRE-binding transcription factor, conferred drought and high-salt tolerance in transgenic plants. Biochem Bioph Res Co 353:299–305

    Article  CAS  Google Scholar 

  • Foyer CH, Noctor G (2003) Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol Plantarum 119:355–364

    Article  CAS  Google Scholar 

  • Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA 103:12987–12992

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Huang B, Jin L, Liu J-Y (2008) Identification and characterization of the novel gene GhDBP2 encoding a DRE-binding protein from cotton (Gossypium hirsutum). J Plant Physiol 165:214–223

    Article  CAS  PubMed  Google Scholar 

  • Islam MS, Wang M-H (2009) Expression of dehydration responsive element-binding protein-3 (DREB3) under different abiotic stresses in tomato. BMB Rep 42:611

    Article  CAS  PubMed  Google Scholar 

  • Kishor PK, Hong Z, Miao G-H, Hu C-AA, Verma DPS (1995) Overexpression of Δ-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394

    CAS  PubMed Central  PubMed  Google Scholar 

  • Koussevitzky S, Nott A, Mockler TC, Hong F, Sachetto-Martins G, Surpin M, Lim J, Mittler R, Chory J (2007) Signals from chloroplasts converge to regulate nuclear gene expression. Sci Signal 316:715

    CAS  Google Scholar 

  • Krishnaswamy S, Verma S, Rahman MH, Kav NN (2011) Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis. Plant Mol Biol 75:107–127

    Article  CAS  PubMed  Google Scholar 

  • Lata C, Prasad M (2011) Role of DREBs in regulation of abiotic stress responses in plants. J Exp Bot 62:4731–4748

    Article  CAS  PubMed  Google Scholar 

  • Lin R-C, Park H-J, Wang H-Y (2008) Role of Arabidopsis RAP2. 4 in regulating light-and ethylene-mediated developmental processes and drought stress tolerance. Mol Plant 1:42–57

    Article  CAS  PubMed  Google Scholar 

  • Liu N, Zhong NQ, Wang GL, Li LJ, Liu XL, He YK, Xia GX (2007) Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens. Planta 226:827–838

    Article  CAS  PubMed  Google Scholar 

  • Liu S, Zheng L, Xue Y, Zhang Q, Wang L, Shou H (2010) Overexpression of OsVP1 and OsNHX1 increases tolerance to drought and salinity in rice. J Plant Physiol 53:444–452

    CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Luo L (2010) Breeding for water-saving and drought-resistance rice (WDR) in China. J Exp Bot 61:3509–3517

    Article  CAS  PubMed  Google Scholar 

  • Mandre M, Tullus H, Kloseiko J (2002) Partitioning of carbohydrates and biomass of needles in Scots pine canopy. Z Naturforsch C 57:296–302

    CAS  PubMed  Google Scholar 

  • Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J (2001) The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J 25:295–303

    Article  CAS  PubMed  Google Scholar 

  • Mukherjee K, Choudhury AR, Gupta B, Gupta S, Sengupta DN (2006) An ABRE-binding factor, OsBZ8, is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice. BMC Plant Biol 6:18

    Article  PubMed Central  PubMed  Google Scholar 

  • Nakashima K, Yamaguchi-Shinozaki K (2005) Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants. Physiol Plantarum 126:62–71

    Article  Google Scholar 

  • Okamuro JK, Caster B, Villarroel R, Van Montagu M, Jofuku KD (1997) The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc Natl Acad Sci USA 94:7076–7081

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Qin Q-L, Liu J-G, Zhang Z, Peng R-H, Xiong A-S, Yao Q-H, Chen JM (2007) Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L. Mol Breeding 19:329–340

    Article  CAS  Google Scholar 

  • Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun 290:998–1009

    Article  CAS  PubMed  Google Scholar 

  • Sharoni AM, Nuruzzaman M, Satoh K, Shimizu T, Kondoh H, Sasaya T, Choi IR, Omura T, Kikuchi S (2011) Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant Cell Physiol 52:344–360

    Article  CAS  PubMed  Google Scholar 

  • Shi H, Ishitani M, Kim C, Zhu J-K (2000) The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc Natl Acad Sci USA 97:6896–6901

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shi H, Lee B-h, Wu S-J, Zhu J-K (2002) Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nat Biotechnol 21:81–85

    Article  PubMed  Google Scholar 

  • Su J, Wu R (2004) Stress-inducible synthesis of proline in transgenic rice confers faster growth under stress conditions than that with constitutive synthesis. Plant Sci 166:941–948

    Article  CAS  Google Scholar 

  • Sun S, Yu JP, Chen F, Zhao TJ, Fang XH, Li YQ, Sui SF (2008) TINY, a dehydration-responsive element (DRE)-binding proteinlike transcription factor connecting the DRE-and ethylene-responsive element-mediated signaling pathways in Arabidopsis. J Biol Chem 283:6261–6271

    Article  CAS  PubMed  Google Scholar 

  • Tang M, Liu X, Deng H, Shen S (2011) Over-expression of JcDREB, a putative AP2/EREBP domain-containing transcription factor gene in woody biodiesel plant Jatropha curcas, enhances salt and freezing tolerance in transgenic Arabidopsis thaliana. Plant Sci 181:623–631

    Article  CAS  PubMed  Google Scholar 

  • Xiu-Hong TIAN, Xue-Ping LI, Hua-Lin ZHOU, Jin-Song ZHANG, Zhi-Zhong GONG, Shou-Yi CHEN (2005) OsDREB4 genes in rice encode AP2-containing proteins that bind specifically to the dehydration-responsive element. J Integr Plant Biol 47:467–476

    Article  CAS  Google Scholar 

  • Verslues PE, Bray EA (2006) Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potentialinduced ABA and proline accumulation. J Exp Bot 57:201–212

    Article  CAS  PubMed  Google Scholar 

  • Xiang Y, Tang N, Du H, Ye H, Xiong L (2008) Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice. Plant Physiol 148:1938–1952

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xue T, Wang D, Zhang S, Ehlting J, Ni F, Jakab S, Zheng C, Zhong Y (2008) Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis. BMC Genomics 9:550

    Article  PubMed Central  PubMed  Google Scholar 

  • Yamaguchi-Shinozaki K, Shinozaki K (1994) A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6:251–264

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yang A, Dai X, Zhang W-H (2012) A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot 63:2541–2556

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61:672–685

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Kirkham M (1994) Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol 35:785–791

    CAS  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhu X, Song F, Xu H (2010) Influence of arbuscular mycorrhiza on lipid peroxidation and antioxidant enzyme activity of maize plants under temperature stress. Mycorrhiza 20:325–332

    Article  CAS  PubMed  Google Scholar 

  • Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J, Xiong AS (2011) Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Mol Biol Rep 38:745–753

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Plant science&Technology, Huazhong Agriculture University, Wuhan, 430070, China

    Ya-Guang Ke, Zhi-Jun Yang & Li-Jun Luo

  2. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Ya-Guang Ke, Shun-Wu Yu, Tian-Fei Li, Jin-Hong Wu, Huan Gao & Li-Jun Luo

  3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    Ya-Ping Fu

Authors
  1. Ya-Guang Ke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhi-Jun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shun-Wu Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tian-Fei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jin-Hong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Huan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ya-Ping Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Li-Jun Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Shun-Wu Yu or Li-Jun Luo.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ke, YG., Yang, ZJ., Yu, SW. et al. Characterization of OsDREB6 responsive to osmotic and cold stresses in rice. J. Plant Biol. 57, 150–161 (2014). https://doi.org/10.1007/s12374-013-0480-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12374-013-0480-0

Keywords

Search

Navigation