Summary
Ethyl methane sulphonate (EMS) is a potential mutagen to induce lincomycin resistance in Capsicum annuum. Mutagenized cotyledons were cultured on shoot regenerating medium containing lincomycin (100 mgl−1). Approximately 14% of regenerated shoots were chlorophyll deficient and about 4% of regenerated shoots were green from mutaganized cotyledons. The regenerated green plants were resistant to lincomycin but sensitive to chloramphenicol, kanamycin, spectinomycin, and streptomycin. Reciprocal crosses were made between resistant and sensitive plants. Inheritance of lincomycin resistance was transmitted as a non-Mendelian trait. Lincomycin resistance is a first selectable and maternally inherited organelle encoded genetic marker described in chili pepper. Such mutants should be useful in designing biochemical selection schemes to recover somatic hybrids and cybrids.
Similar content being viewed by others
References
CorGlas, J. C.; Jangsma, K. C.; Nijkamp, H. J. J., et al. The induction of lincomycin resistance in Lycopersicon peruvianum and Lycopersicon esculentum. Plant Sci. 70:231–241; 1990.
Cseplo, A.; Eigel, L.; Horvath, G. V., et al. Subcellular location of lincomycin resistance in Nicotiana mutants. Mol. & Gen. Genet. 232:392–396; 1992.
Cseplo, A.; Etzold, T.; Schell, J., et al. Point mutations in the 23 S rRNA gene of 4-lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation. Mol. & Gen. Genet. 214:295–299; 1988.
Cseplo, A.; Maliga, P. Lincomycin resistance a new type of maternally inherited mutation in Nicotiana plumbaginifolia. Curr. Genet. 6:105–109; 1982.
Cseplo, A; Maliga, P. Large-scale isolation of maternally inherited lincomycin resistance mutations in diploid Nicotiana plumbaginifolia protoplast cultures, Mol. & Gen. Genet. 196:407–412; 1984.
Cseplo, A.; Medgyesy, P.; Hideg, E., et al. Triazine-resistant Nicotiana mutants from photo mixotrophic cell cultures. Mol. & Gen. Genet. 200:508–510; 1985.
Dahlberg, A. E. The functional role of ribosomal RNA in protein synthesis. Cell. 57(4):525–529; 1989.
Dix, P. J.; Mc Kinely, C. P.; McCabe, P. F. Antibiotic resistant mutants of Solanum nigrum. In: Nijkamp, H. J. J.; Van der Plas L. H. W.; Van Aartrij, K. J., eds. Progress in plant cellular and molecular biology. Dordrecht, Netherlands: Kluwer Academic Publishers; 1990:169–175.
Farooqui, A. M. Studies on tissue culture, mutant selection and root-knot nematode infestation in Solanum melongena (egg plant). Warangal, India: Kakatiya University; 1995. Ph.D. Thesis; 89–94.
Fluhr, R.; Aviv, D.; Galun, E., et al. Efficient induction and selection of chloroplast-encoded antibiotic resistant mutants in Nicotiana. Proc. Natl. Acad. Sci. USA 82:1485–1489; 1985.
Golds, T.; Maliga, P.; Koop, H.-U. Stable plasmid transformation in PEG-treated protoplasts of Nicotiana tabacum. Bio/technology 11:95; 1993.
Hosticka, L. P.; Hanson, M. R. Induction of plastid mutations in tomatoes by nitrosomethylurea. J. Hered. 75:242–246; 1984.
Jansen, C. E; Snel, E. A. M.; Akerboom, M. J. E., et al. Induction of streptomycin resistance in wild tomato Lycopersicon peruvianum. Mol. & Gen. Genet. 220:261–268; 1990.
McCabe, P. F.; Timmons, A. M.; Dix, P. J. A simple procedure for the isolation of streptomycin-resistant plants in Solanaceae. Mol. & Gen. Genet. 216:132–137; 1989.
McCabe, P. F.; Cseplo, A.; Timmons, A. M., et al. Selection of chloroplast mutants. In: Pollard, J. W.; Walker, J. M., eds. Methods in molecular biology. Vol. 6. Plant cell and tissue culture. Totowa, NJ: Humana Press; 1990:467–475.
Medgyesy, P. Selection and analysis of cytoplasmic hybrids. In: Dix, P. J., ed. Plant cell line selection. Weinheim, Germany: VCH; 1990:287–316.
Medgyesy, P.; Fejes, E.; Maliga, P. Interspecific chloroplast recombination in Nicotiana somatic hybrids. Proc. Natl. Acad. Sci. USA 82:6960–6964; 1985.
Medgyesy, P.; Menczel, L.; Maliga, P. The use of cytoplasmic streptomycin-resistance; chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids. Theor. Appl. Genet. 59:191–195; 1980.
Menczel, L.; Polsby, L. S.; Steinback, K. E., et al. Fusion mediated transfer of triazine-resistant chloroplasts: characterization of Nicotiana tabacum cybrid plants. Mol. & Gen. Genet. 205:201–205; 1986.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473–497; 1962.
Perl, A.; Aviv, D.; Galun, E. Protoplast fusion mediated transfer of oligomycin resistance from Nicotiana sylvestris to Solanum tuberosum by intergenetic cybridization. Mol. & Gen. Genet. 225:11–16; 1991.
Rao, A. V.; Farooqui, A.; JayaSree, T., et al. EMS-induced streptomycin-resistance in Solanum melongena. Theor. Appl. Genet. 87:527–530; 1993.
Sadanandam, A.; Farooqui, A. M. Induction and selection of lincomycin-resistant plants in Solanum melongena. Plant Sci. 79:234–237; 1991.
Svab, Z.; Hajdukieniiz, P.; Maliga, P. Stable transformation of plastids in higher plants. Proc. Natl. Acad. Sci. USA 87:8526–8530; 1990.
Svab, Z.; Maliga, P. Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor. Appl. Genet. 72:637–643; 1986.
To. K.-Y.; Chen, C.-C.; Lai, Y.-K. Isolation and characterization of streptomycin-resistant mutants in Nicotiana plumbaginifolia. Theor. Appl. Genet. 78:81–86; 1989.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Subhash, K., Venkataiah, P. & Bhaskar, P. EMS-induced lincomycin resistance in red pepper (Capsicum annuum L.). In Vitro Cell.Dev.Biol.-Plant 33, 285–287 (1997). https://doi.org/10.1007/s11627-997-0051-5
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-997-0051-5